A nanofluidic device for single molecule studies with in situ control of environmental solution conditions
We report an approach to study the in situ conformational response of single biomolecules such as DNA to a change in environmental solution conditions. These conditions are, for example, the composition of the buffer or the presence of protein. For this purpose, we designed and fabricated a nanoflui...
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| Format: | Article |
| Language: | en_US |
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Royal Society of Chemistry, The
2014
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| Online Access: | http://hdl.handle.net/1721.1/91270 |
| _version_ | 1826210631735312384 |
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| author | Zhang, Ce Jiang, Kai Liu, Fan Doyle, Patrick S. van Kan, Jeroen A. van der Maarel, Johan R. C. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Zhang, Ce Jiang, Kai Liu, Fan Doyle, Patrick S. van Kan, Jeroen A. van der Maarel, Johan R. C. |
| author_sort | Zhang, Ce |
| collection | MIT |
| description | We report an approach to study the in situ conformational response of single biomolecules such as DNA to a change in environmental solution conditions. These conditions are, for example, the composition of the buffer or the presence of protein. For this purpose, we designed and fabricated a nanofluidic device featuring two arrays of parallel nanochannels in a perpendicular configuration. The cross-sections of the channels are rectangular with a diameter down to 175 nm. These lab-on-a-chip devices were made of polydimethylsiloxane (PDMS) cast on a high quality master stamp, obtained by proton beam writing and UV lithography. Biomolecules can be inserted into the device through the array of channels in one direction, whereas the buffer can be exchanged through the intersecting array of channels in the other direction. A buffer exchange time inside the grid of nanochannels of less than one second was measured by monitoring the conductivity of salt solutions. The exchange time of a protein was typically a few seconds, as determined by imaging the influx of fluorescence labelled protamine. We demonstrate the functionality of the device by investigating the compaction of DNA by protamine and the unpacking of pre-compacted DNA through an increase in the concentration of salt. |
| first_indexed | 2024-09-23T14:53:01Z |
| format | Article |
| id | mit-1721.1/91270 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:53:01Z |
| publishDate | 2014 |
| publisher | Royal Society of Chemistry, The |
| record_format | dspace |
| spelling | mit-1721.1/912702022-10-01T23:06:22Z A nanofluidic device for single molecule studies with in situ control of environmental solution conditions Zhang, Ce Jiang, Kai Liu, Fan Doyle, Patrick S. van Kan, Jeroen A. van der Maarel, Johan R. C. Massachusetts Institute of Technology. Department of Chemical Engineering Doyle, Patrick S. We report an approach to study the in situ conformational response of single biomolecules such as DNA to a change in environmental solution conditions. These conditions are, for example, the composition of the buffer or the presence of protein. For this purpose, we designed and fabricated a nanofluidic device featuring two arrays of parallel nanochannels in a perpendicular configuration. The cross-sections of the channels are rectangular with a diameter down to 175 nm. These lab-on-a-chip devices were made of polydimethylsiloxane (PDMS) cast on a high quality master stamp, obtained by proton beam writing and UV lithography. Biomolecules can be inserted into the device through the array of channels in one direction, whereas the buffer can be exchanged through the intersecting array of channels in the other direction. A buffer exchange time inside the grid of nanochannels of less than one second was measured by monitoring the conductivity of salt solutions. The exchange time of a protein was typically a few seconds, as determined by imaging the influx of fluorescence labelled protamine. We demonstrate the functionality of the device by investigating the compaction of DNA by protamine and the unpacking of pre-compacted DNA through an increase in the concentration of salt. Singapore-MIT Alliance for Research and Technology Singapore. Ministry of Education (Grant R-144-000-270-112) Singapore. Ministry of Education (Grant R-144-000-312-112) National Science Foundation (U.S.) (Grant CBET-0852235) 2014-11-03T15:17:04Z 2014-11-03T15:17:04Z 2013-04 2013-02 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/91270 Zhang, Ce, Kai Jiang, Fan Liu, Patrick S. Doyle, Jeroen A. van Kan, and Johan R. C. van der Maarel. “A Nanofluidic Device for Single Molecule Studies with in Situ Control of Environmental Solution Conditions.” Lab Chip 13, no. 14 (2013): 2821. en_US http://dx.doi.org/10.1039/c3lc50233c Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Royal Society of Chemistry, The MIT web domain |
| spellingShingle | Zhang, Ce Jiang, Kai Liu, Fan Doyle, Patrick S. van Kan, Jeroen A. van der Maarel, Johan R. C. A nanofluidic device for single molecule studies with in situ control of environmental solution conditions |
| title | A nanofluidic device for single molecule studies with in situ control of environmental solution conditions |
| title_full | A nanofluidic device for single molecule studies with in situ control of environmental solution conditions |
| title_fullStr | A nanofluidic device for single molecule studies with in situ control of environmental solution conditions |
| title_full_unstemmed | A nanofluidic device for single molecule studies with in situ control of environmental solution conditions |
| title_short | A nanofluidic device for single molecule studies with in situ control of environmental solution conditions |
| title_sort | nanofluidic device for single molecule studies with in situ control of environmental solution conditions |
| url | http://hdl.handle.net/1721.1/91270 |
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