Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device
The ability to straightforwardly deliver engineered nanoparticles into the cell cytosol with high viability will vastly expand the range of biological applications. Nanoparticles could potentially be used as delivery vehicles or as fluorescent sensors to probe the cell. In particular, quantum dots (...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2014
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| Online Access: | http://hdl.handle.net/1721.1/91465 https://orcid.org/0000-0003-2220-4365 https://orcid.org/0000-0001-7192-580X https://orcid.org/0000-0003-4255-0492 |
| _version_ | 1811093378671050752 |
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| author | Lee, Jungmin Sharei, Armon Reza Sim, Woo Young Adamo, Andrea Langer, Robert S Jensen, Klavs F Bawendi, Moungi G |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Lee, Jungmin Sharei, Armon Reza Sim, Woo Young Adamo, Andrea Langer, Robert S Jensen, Klavs F Bawendi, Moungi G |
| author_sort | Lee, Jungmin |
| collection | MIT |
| description | The ability to straightforwardly deliver engineered nanoparticles into the cell cytosol with high viability will vastly expand the range of biological applications. Nanoparticles could potentially be used as delivery vehicles or as fluorescent sensors to probe the cell. In particular, quantum dots (QDs) may be used to illuminate cytosolic proteins for long-term microscopy studies. Whereas recent advances have been successful in specifically labeling proteins with QDs on the cell membrane, cytosolic delivery of QDs into live cells has remained challenging. In this report, we demonstrate high throughput delivery of QDs into live cell cytoplasm using an uncomplicated microfluidic device while maintaining cell viabilities of 80–90%. We verify that the nanoparticle surface interacts with the cytosolic environment and that the QDs remain nonaggregated so that single QDs can be observed. |
| first_indexed | 2024-09-23T15:44:18Z |
| format | Article |
| id | mit-1721.1/91465 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:44:18Z |
| publishDate | 2014 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/914652022-10-02T03:45:36Z Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device Lee, Jungmin Sharei, Armon Reza Sim, Woo Young Adamo, Andrea Langer, Robert S Jensen, Klavs F Bawendi, Moungi G Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Chemistry Koch Institute for Integrative Cancer Research at MIT Lee, Jungmin Sharei, Armon Reza Sim, Woo Young Adamo, Andrea Langer, Robert Jensen, Klavs F. Bawendi, Moungi G. The ability to straightforwardly deliver engineered nanoparticles into the cell cytosol with high viability will vastly expand the range of biological applications. Nanoparticles could potentially be used as delivery vehicles or as fluorescent sensors to probe the cell. In particular, quantum dots (QDs) may be used to illuminate cytosolic proteins for long-term microscopy studies. Whereas recent advances have been successful in specifically labeling proteins with QDs on the cell membrane, cytosolic delivery of QDs into live cells has remained challenging. In this report, we demonstrate high throughput delivery of QDs into live cell cytoplasm using an uncomplicated microfluidic device while maintaining cell viabilities of 80–90%. We verify that the nanoparticle surface interacts with the cytosolic environment and that the QDs remain nonaggregated so that single QDs can be observed. National Institutes of Health (U.S.) (NIH grant 5-U54-CA151884-03) National Institutes of Health (U.S.) (NIH grant 5R01CA126642-02) National Institutes of Health (U.S.) (NIH grant RC1 EB011187-02) Massachusetts Institute of Technology. Biophysical Instrumentation Facility (NSF-0070319) Massachusetts Institute of Technology. Biophysical Instrumentation Facility (NIH GM68762) Massachusetts Institute of Technology. Department of Chemistry Instrumentation Facility (CHE- 9808061) Massachusetts Institute of Technology. Department of Chemistry Instrumentation Facility (DBI-9729592) National Cancer Institute (U.S.) (P30-CA14051) 2014-11-05T17:31:27Z 2014-11-05T17:31:27Z 2012-12 2012-11 Article http://purl.org/eprint/type/JournalArticle 1530-6984 1530-6992 http://hdl.handle.net/1721.1/91465 Lee, Jungmin, Armon Sharei, Woo Young Sim, Andrea Adamo, Robert Langer, Klavs F. Jensen, and Moungi G. Bawendi. “Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device.” Nano Lett. 12, no. 12 (December 12, 2012): 6322–6327. https://orcid.org/0000-0003-2220-4365 https://orcid.org/0000-0001-7192-580X https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.1021/nl303421h Nano Letters Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Lee, Jungmin Sharei, Armon Reza Sim, Woo Young Adamo, Andrea Langer, Robert S Jensen, Klavs F Bawendi, Moungi G Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device |
| title | Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device |
| title_full | Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device |
| title_fullStr | Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device |
| title_full_unstemmed | Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device |
| title_short | Nonendocytic Delivery of Functional Engineered Nanoparticles into the Cytoplasm of Live Cells Using a Novel, High-Throughput Microfluidic Device |
| title_sort | nonendocytic delivery of functional engineered nanoparticles into the cytoplasm of live cells using a novel high throughput microfluidic device |
| url | http://hdl.handle.net/1721.1/91465 https://orcid.org/0000-0003-2220-4365 https://orcid.org/0000-0001-7192-580X https://orcid.org/0000-0003-4255-0492 |
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