Polymer multilayer tattooing for enhanced DNA vaccination
DNA vaccines have many potential benefits but have failed to generate robust immune responses in humans. Recently, methods such as in vivo electroporation have demonstrated improved performance, but an optimal strategy for safe, reproducible, and pain-free DNA vaccination remains elusive. Here we re...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/91478 |
| _version_ | 1826217682248138752 |
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| author | DeMuth, Peter Charles Min, Younjin Huang, Bonnie Kramer, Joshua A. Miller, Andrew D. Barouch, Dan H. Hammond, Paula T. Irvine, Darrell J. |
| author2 | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies |
| author_facet | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies DeMuth, Peter Charles Min, Younjin Huang, Bonnie Kramer, Joshua A. Miller, Andrew D. Barouch, Dan H. Hammond, Paula T. Irvine, Darrell J. |
| author_sort | DeMuth, Peter Charles |
| collection | MIT |
| description | DNA vaccines have many potential benefits but have failed to generate robust immune responses in humans. Recently, methods such as in vivo electroporation have demonstrated improved performance, but an optimal strategy for safe, reproducible, and pain-free DNA vaccination remains elusive. Here we report an approach for rapid implantation of vaccine-loaded polymer films carrying DNA, immune-stimulatory RNA, and biodegradable polycations into the immune-cell-rich epidermis, using microneedles coated with releasable polyelectrolyte multilayers. Films transferred into the skin following brief microneedle application promoted local transfection and controlled the persistence of DNA and adjuvants in the skin from days to weeks, with kinetics determined by the film composition. These ‘multilayer tattoo’ DNA vaccines induced immune responses against a model HIV antigen comparable to electroporation in mice, enhanced memory T-cell generation, and elicited 140-fold higher gene expression in non-human primate skin than intradermal DNA injection, indicating the potential of this strategy for enhancing DNA vaccination. |
| first_indexed | 2024-09-23T17:07:29Z |
| format | Article |
| id | mit-1721.1/91478 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T17:07:29Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/914782022-10-03T10:34:38Z Polymer multilayer tattooing for enhanced DNA vaccination DeMuth, Peter Charles Min, Younjin Huang, Bonnie Kramer, Joshua A. Miller, Andrew D. Barouch, Dan H. Hammond, Paula T. Irvine, Darrell J. Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Ragon Institute of MGH, MIT and Harvard Koch Institute for Integrative Cancer Research at MIT Hammond, Paula T. DeMuth, Peter Charles Min, Younjin Huang, Bonnie Kramer, Joshua A. Miller, Andrew D. Barouch, Dan H. Hammond, Paula T. Irvine, Darrell J. DNA vaccines have many potential benefits but have failed to generate robust immune responses in humans. Recently, methods such as in vivo electroporation have demonstrated improved performance, but an optimal strategy for safe, reproducible, and pain-free DNA vaccination remains elusive. Here we report an approach for rapid implantation of vaccine-loaded polymer films carrying DNA, immune-stimulatory RNA, and biodegradable polycations into the immune-cell-rich epidermis, using microneedles coated with releasable polyelectrolyte multilayers. Films transferred into the skin following brief microneedle application promoted local transfection and controlled the persistence of DNA and adjuvants in the skin from days to weeks, with kinetics determined by the film composition. These ‘multilayer tattoo’ DNA vaccines induced immune responses against a model HIV antigen comparable to electroporation in mice, enhanced memory T-cell generation, and elicited 140-fold higher gene expression in non-human primate skin than intradermal DNA injection, indicating the potential of this strategy for enhancing DNA vaccination. Howard Hughes Medical Institute (Investigator) Ragon Institute of MGH, MIT, and Harvard National Institutes of Health (U.S.) (NIH AI095109) United States. Dept. of Defense. Institute for Soldier Nanotechnologies (contract W911NF-07-D-0004) United States. Dept. of Defense. Institute for Soldier Nanotechnologies (contract W911NF-07-0004) 2014-11-06T20:12:31Z 2014-11-06T20:12:31Z 2013-01 2012-06 Article http://purl.org/eprint/type/JournalArticle 1476-1122 1476-4660 http://hdl.handle.net/1721.1/91478 DeMuth, Peter C., Younjin Min, Bonnie Huang, Joshua A. Kramer, Andrew D. Miller, Dan H. Barouch, Paula T. Hammond, and Darrell J. Irvine. “Polymer Multilayer Tattooing for Enhanced DNA Vaccination.” Nature Materials 12, no. 4 (January 27, 2013): 367–376. en_US http://dx.doi.org/10.1038/nmat3550 Nature Materials Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | DeMuth, Peter Charles Min, Younjin Huang, Bonnie Kramer, Joshua A. Miller, Andrew D. Barouch, Dan H. Hammond, Paula T. Irvine, Darrell J. Polymer multilayer tattooing for enhanced DNA vaccination |
| title | Polymer multilayer tattooing for enhanced DNA vaccination |
| title_full | Polymer multilayer tattooing for enhanced DNA vaccination |
| title_fullStr | Polymer multilayer tattooing for enhanced DNA vaccination |
| title_full_unstemmed | Polymer multilayer tattooing for enhanced DNA vaccination |
| title_short | Polymer multilayer tattooing for enhanced DNA vaccination |
| title_sort | polymer multilayer tattooing for enhanced dna vaccination |
| url | http://hdl.handle.net/1721.1/91478 |
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