Flow-Through Comb Electroporation Device for Delivery of Macromolecules
We present a microfluidic electroporation device with a comb electrode layout fabricated in polydimethylsiloxane (PMDS) and glass. Characterization experiments with HeLa cells and fluorescent dextran show efficient delivery (~95%) with low toxicity (cell viability ~85%) as well as rapid pore closure...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2014
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| Online Access: | http://hdl.handle.net/1721.1/91542 https://orcid.org/0000-0001-7192-580X |
| _version_ | 1826207720356708352 |
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| author | Adamo, Andrea Arione, Alessandro Sharei, Armon Reza Jensen, Klavs F |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Adamo, Andrea Arione, Alessandro Sharei, Armon Reza Jensen, Klavs F |
| author_sort | Adamo, Andrea |
| collection | MIT |
| description | We present a microfluidic electroporation device with a comb electrode layout fabricated in polydimethylsiloxane (PMDS) and glass. Characterization experiments with HeLa cells and fluorescent dextran show efficient delivery (~95%) with low toxicity (cell viability ~85%) as well as rapid pore closure after electroporation. The activity of delivered molecules is also verified by silencing RNA (siRNA) studies that demonstrate gene knockdown in GFP expressing cells. This simple, scalable approach to microfluidic, flow-through electroporation could facilitate the integration of electroporation modules within cell analysis devices that perform multiple operations. |
| first_indexed | 2024-09-23T13:53:55Z |
| format | Article |
| id | mit-1721.1/91542 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:53:55Z |
| publishDate | 2014 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/915422022-09-28T16:55:23Z Flow-Through Comb Electroporation Device for Delivery of Macromolecules Adamo, Andrea Arione, Alessandro Sharei, Armon Reza Jensen, Klavs F Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Adamo, Andrea Arione, Alessandro Sharei, Armon Reza Jensen, Klavs F. We present a microfluidic electroporation device with a comb electrode layout fabricated in polydimethylsiloxane (PMDS) and glass. Characterization experiments with HeLa cells and fluorescent dextran show efficient delivery (~95%) with low toxicity (cell viability ~85%) as well as rapid pore closure after electroporation. The activity of delivered molecules is also verified by silencing RNA (siRNA) studies that demonstrate gene knockdown in GFP expressing cells. This simple, scalable approach to microfluidic, flow-through electroporation could facilitate the integration of electroporation modules within cell analysis devices that perform multiple operations. National Institutes of Health (U.S.) (Grant RC1 EB011187-02) National Cancer Institute (U.S.) (Cancer Center Support (Core) Grant P30-CA14051) 2014-11-13T18:24:42Z 2014-11-13T18:24:42Z 2012-12 2012-10 Article http://purl.org/eprint/type/JournalArticle 0003-2700 1520-6882 http://hdl.handle.net/1721.1/91542 Adamo, Andrea, Alessandro Arione, Armon Sharei, and Klavs F. Jensen. “Flow-Through Comb Electroporation Device for Delivery of Macromolecules.” Analytical Chemistry 85, no. 3 (February 5, 2013): 1637–1641. https://orcid.org/0000-0001-7192-580X en_US http://dx.doi.org/10.1021/ac302887a Analytical Chemistry Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Adamo, Andrea Arione, Alessandro Sharei, Armon Reza Jensen, Klavs F Flow-Through Comb Electroporation Device for Delivery of Macromolecules |
| title | Flow-Through Comb Electroporation Device for Delivery of Macromolecules |
| title_full | Flow-Through Comb Electroporation Device for Delivery of Macromolecules |
| title_fullStr | Flow-Through Comb Electroporation Device for Delivery of Macromolecules |
| title_full_unstemmed | Flow-Through Comb Electroporation Device for Delivery of Macromolecules |
| title_short | Flow-Through Comb Electroporation Device for Delivery of Macromolecules |
| title_sort | flow through comb electroporation device for delivery of macromolecules |
| url | http://hdl.handle.net/1721.1/91542 https://orcid.org/0000-0001-7192-580X |
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