CD8[superscript +] T Cells from Mice Transnuclear for a TCR that Recognizes a Single H-2K[superscript b]-Restricted MHV68 Epitope Derived from gB-ORF8 Help Control Infection
To study the CD8[superscript +] T cell response against a mouse γ-herpes virus, we generated K[superscript b]-MHV-68-ORF8[superscript 604–612]RAG[superscript −/−] CD8[superscript +] T cell receptor transnuclear (TN) mice as a source of virus-specific CD8[superscript +] T cells. K[superscript b]-ORF8...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/91544 https://orcid.org/0000-0002-1090-6071 |
| _version_ | 1826195957653438464 |
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| author | Sehrawat, Sharvan Kirak, Oktay Koenig, Paul-Albert Isaacson, Marisa K. Marques, Sofia Bozkurt, Gunes Simas, J. Pedro Jaenisch, Rudolf Ploegh, Hidde |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Sehrawat, Sharvan Kirak, Oktay Koenig, Paul-Albert Isaacson, Marisa K. Marques, Sofia Bozkurt, Gunes Simas, J. Pedro Jaenisch, Rudolf Ploegh, Hidde |
| author_sort | Sehrawat, Sharvan |
| collection | MIT |
| description | To study the CD8[superscript +] T cell response against a mouse γ-herpes virus, we generated K[superscript b]-MHV-68-ORF8[superscript 604–612]RAG[superscript −/−] CD8[superscript +] T cell receptor transnuclear (TN) mice as a source of virus-specific CD8[superscript +] T cells. K[superscript b]-ORF8-Tet[superscript +] CD8[superscript +] T cells, expanded in the course of a resolving MHV-68 infection, served as a source of nucleus donors. Various in vivo and ex vivo assay criteria demonstrated the fine specificity and functionality of TN cells. TN cells proliferated extensively in response to viral infection, helped control viral burden, and exhibited a phenotype similar to that of endogenous K[superscript b]-ORF8-Tet[superscript +] cells. When compared to OT-1 cells, TN cells displayed distinct properties in response to lymphopenia and cognate antigen stimulation, which may be attributable to the affinity of the TCR expressed by the TN cells. The availability of MHV-68-specific CD8[superscript +] TCR TN mice provides a new tool for investigating aspects of host-pathogen interactions unique to γ-herpes viruses. |
| first_indexed | 2024-09-23T10:18:31Z |
| format | Article |
| id | mit-1721.1/91544 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:18:31Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/915442022-09-30T20:17:27Z CD8[superscript +] T Cells from Mice Transnuclear for a TCR that Recognizes a Single H-2K[superscript b]-Restricted MHV68 Epitope Derived from gB-ORF8 Help Control Infection Sehrawat, Sharvan Kirak, Oktay Koenig, Paul-Albert Isaacson, Marisa K. Marques, Sofia Bozkurt, Gunes Simas, J. Pedro Jaenisch, Rudolf Ploegh, Hidde Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Jaenisch, Rudolf Ploegh, Hidde To study the CD8[superscript +] T cell response against a mouse γ-herpes virus, we generated K[superscript b]-MHV-68-ORF8[superscript 604–612]RAG[superscript −/−] CD8[superscript +] T cell receptor transnuclear (TN) mice as a source of virus-specific CD8[superscript +] T cells. K[superscript b]-ORF8-Tet[superscript +] CD8[superscript +] T cells, expanded in the course of a resolving MHV-68 infection, served as a source of nucleus donors. Various in vivo and ex vivo assay criteria demonstrated the fine specificity and functionality of TN cells. TN cells proliferated extensively in response to viral infection, helped control viral burden, and exhibited a phenotype similar to that of endogenous K[superscript b]-ORF8-Tet[superscript +] cells. When compared to OT-1 cells, TN cells displayed distinct properties in response to lymphopenia and cognate antigen stimulation, which may be attributable to the affinity of the TCR expressed by the TN cells. The availability of MHV-68-specific CD8[superscript +] TCR TN mice provides a new tool for investigating aspects of host-pathogen interactions unique to γ-herpes viruses. National Institutes of Health (U.S.) 2014-11-13T18:31:47Z 2014-11-13T18:31:47Z 2012-04 2012-03 Article http://purl.org/eprint/type/JournalArticle 22111247 http://hdl.handle.net/1721.1/91544 Sehrawat, Sharvan, Oktay Kirak, Paul-Albert Koenig, Marisa K. Isaacson, Sofia Marques, Gunes Bozkurt, J. Pedro Simas, Rudolph Jaenisch, and Hidde L. Ploegh. “CD8+ T Cells from Mice Transnuclear for a TCR That Recognizes a Single H-2Kb-Restricted MHV68 Epitope Derived from gB-ORF8 Help Control Infection.” Cell Reports 1, no. 5 (May 2012): 461–471. https://orcid.org/0000-0002-1090-6071 en_US http://dx.doi.org/10.1016/j.celrep.2012.03.009 Cell Reports Creative Commons Attribution http://creativecommons.org/licenses/by-nc-nd/3.0/ application/pdf Elsevier Elsevier |
| spellingShingle | Sehrawat, Sharvan Kirak, Oktay Koenig, Paul-Albert Isaacson, Marisa K. Marques, Sofia Bozkurt, Gunes Simas, J. Pedro Jaenisch, Rudolf Ploegh, Hidde CD8[superscript +] T Cells from Mice Transnuclear for a TCR that Recognizes a Single H-2K[superscript b]-Restricted MHV68 Epitope Derived from gB-ORF8 Help Control Infection |
| title | CD8[superscript +] T Cells from Mice Transnuclear for a TCR that Recognizes a Single H-2K[superscript b]-Restricted MHV68 Epitope Derived from gB-ORF8 Help Control Infection |
| title_full | CD8[superscript +] T Cells from Mice Transnuclear for a TCR that Recognizes a Single H-2K[superscript b]-Restricted MHV68 Epitope Derived from gB-ORF8 Help Control Infection |
| title_fullStr | CD8[superscript +] T Cells from Mice Transnuclear for a TCR that Recognizes a Single H-2K[superscript b]-Restricted MHV68 Epitope Derived from gB-ORF8 Help Control Infection |
| title_full_unstemmed | CD8[superscript +] T Cells from Mice Transnuclear for a TCR that Recognizes a Single H-2K[superscript b]-Restricted MHV68 Epitope Derived from gB-ORF8 Help Control Infection |
| title_short | CD8[superscript +] T Cells from Mice Transnuclear for a TCR that Recognizes a Single H-2K[superscript b]-Restricted MHV68 Epitope Derived from gB-ORF8 Help Control Infection |
| title_sort | cd8 superscript t cells from mice transnuclear for a tcr that recognizes a single h 2k superscript b restricted mhv68 epitope derived from gb orf8 help control infection |
| url | http://hdl.handle.net/1721.1/91544 https://orcid.org/0000-0002-1090-6071 |
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