Maximal Force Characteristics of the Ca[superscript 2+]-Powered Actuator of Vorticella convallaria
The millisecond stalk contraction of the sessile ciliate Vorticella convallaria is powered by energy from Ca[superscript 2+] binding to generate contractile forces of ∼10 nN. Its contractile organelle, the spasmoneme, generates higher contractile force under increased stall resistances. By applying...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/91548 https://orcid.org/0000-0002-4614-251X https://orcid.org/0000-0001-9142-3030 |
| _version_ | 1811088644092461056 |
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| author | Ryu, Sangjin Lang, Matthew J. Matsudaira, Paul T. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Ryu, Sangjin Lang, Matthew J. Matsudaira, Paul T. |
| author_sort | Ryu, Sangjin |
| collection | MIT |
| description | The millisecond stalk contraction of the sessile ciliate Vorticella convallaria is powered by energy from Ca[superscript 2+] binding to generate contractile forces of ∼10 nN. Its contractile organelle, the spasmoneme, generates higher contractile force under increased stall resistances. By applying viscous drag force to contracting V. convallaria in a microfluidic channel, we observed that the mechanical force and work of the spasmoneme depended on the stalk length, i.e., the maximum tension (150–350 nN) and work linearly depended on the stalk length (~2.5 nN and ~30 fJ per 1 μm of the stalk). This stalk-length dependency suggests that motor units of the spasmoneme may be organized in such a way that the mechanical force and work of each unit cumulate in series along the spasmoneme. |
| first_indexed | 2024-09-23T14:05:15Z |
| format | Article |
| id | mit-1721.1/91548 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:05:15Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/915482022-09-28T18:18:50Z Maximal Force Characteristics of the Ca[superscript 2+]-Powered Actuator of Vorticella convallaria Ryu, Sangjin Lang, Matthew J. Matsudaira, Paul T. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Mechanical Engineering Whitehead Institute for Biomedical Research Lang, Matthew J. Matsudaira, Paul T. Ryu, Sangjin The millisecond stalk contraction of the sessile ciliate Vorticella convallaria is powered by energy from Ca[superscript 2+] binding to generate contractile forces of ∼10 nN. Its contractile organelle, the spasmoneme, generates higher contractile force under increased stall resistances. By applying viscous drag force to contracting V. convallaria in a microfluidic channel, we observed that the mechanical force and work of the spasmoneme depended on the stalk length, i.e., the maximum tension (150–350 nN) and work linearly depended on the stalk length (~2.5 nN and ~30 fJ per 1 μm of the stalk). This stalk-length dependency suggests that motor units of the spasmoneme may be organized in such a way that the mechanical force and work of each unit cumulate in series along the spasmoneme. DuPont (Firm) United States. Army Research Office (Institute for Collaborative Biotechnologies Grant DAAD19-03-D-0004) 2014-11-13T18:57:27Z 2014-11-13T18:57:27Z 2012-09 2012-02 Article http://purl.org/eprint/type/JournalArticle 00063495 1542-0086 http://hdl.handle.net/1721.1/91548 Ryu, Sangjin, Matthew J. Lang, and Paul Matsudaira. “Maximal Force Characteristics of the Ca2+-Powered Actuator of Vorticella Convallaria.” Biophysical Journal 103, no. 5 (September 2012): 860–867. © 2012 Biophysical Society https://orcid.org/0000-0002-4614-251X https://orcid.org/0000-0001-9142-3030 en_US http://dx.doi.org/10.1016/j.bpj.2012.07.038 Biophysical Journal Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier |
| spellingShingle | Ryu, Sangjin Lang, Matthew J. Matsudaira, Paul T. Maximal Force Characteristics of the Ca[superscript 2+]-Powered Actuator of Vorticella convallaria |
| title | Maximal Force Characteristics of the Ca[superscript 2+]-Powered Actuator of Vorticella convallaria |
| title_full | Maximal Force Characteristics of the Ca[superscript 2+]-Powered Actuator of Vorticella convallaria |
| title_fullStr | Maximal Force Characteristics of the Ca[superscript 2+]-Powered Actuator of Vorticella convallaria |
| title_full_unstemmed | Maximal Force Characteristics of the Ca[superscript 2+]-Powered Actuator of Vorticella convallaria |
| title_short | Maximal Force Characteristics of the Ca[superscript 2+]-Powered Actuator of Vorticella convallaria |
| title_sort | maximal force characteristics of the ca superscript 2 powered actuator of vorticella convallaria |
| url | http://hdl.handle.net/1721.1/91548 https://orcid.org/0000-0002-4614-251X https://orcid.org/0000-0001-9142-3030 |
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