Pervasive Synaptic Branch Removal in the Mammalian Neuromuscular System at Birth
Using light and serial electron microscopy, we show profound refinements in motor axonal branching and synaptic connectivity before and after birth. Embryonic axons become maximally connected just before birth when they innervate ~10-fold more muscle fibers than in maturity. In some developing muscl...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/91553 |
| _version_ | 1811091494167117824 |
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| author | Tapia, Juan C. Wylie, John D. Kasthuri, Narayanan Hayworth, Kenneth J. Schalek, Richard Berger, Daniel R. Guatimosim, Cristina Seung, H. Sebastian Lichtman, Jeff W. |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Tapia, Juan C. Wylie, John D. Kasthuri, Narayanan Hayworth, Kenneth J. Schalek, Richard Berger, Daniel R. Guatimosim, Cristina Seung, H. Sebastian Lichtman, Jeff W. |
| author_sort | Tapia, Juan C. |
| collection | MIT |
| description | Using light and serial electron microscopy, we show profound refinements in motor axonal branching and synaptic connectivity before and after birth. Embryonic axons become maximally connected just before birth when they innervate ~10-fold more muscle fibers than in maturity. In some developing muscles, axons innervate almost every muscle fiber. At birth, each neuromuscular junction is coinnervated by approximately ten highly intermingled axons (versus one in adults). Extensive die off of terminal branches occurs during the first several postnatal days, leading to much sparser arbors that still span the same territory. Despite the extensive pruning, total axoplasm per neuron increases as axons elongate, thicken, and add more synaptic release sites on their remaining targets. Motor axons therefore initially establish weak connections with nearly all available postsynaptic targets but, beginning at birth, massively redistribute synaptic resources, concentrating many more synaptic sites on many fewer muscle fibers. Analogous changes in connectivity may occur in the CNS. |
| first_indexed | 2024-09-23T15:03:15Z |
| format | Article |
| id | mit-1721.1/91553 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:03:15Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/915532022-09-29T12:18:36Z Pervasive Synaptic Branch Removal in the Mammalian Neuromuscular System at Birth Tapia, Juan C. Wylie, John D. Kasthuri, Narayanan Hayworth, Kenneth J. Schalek, Richard Berger, Daniel R. Guatimosim, Cristina Seung, H. Sebastian Lichtman, Jeff W. Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Berger, Daniel R. Seung, H. Sebastian Using light and serial electron microscopy, we show profound refinements in motor axonal branching and synaptic connectivity before and after birth. Embryonic axons become maximally connected just before birth when they innervate ~10-fold more muscle fibers than in maturity. In some developing muscles, axons innervate almost every muscle fiber. At birth, each neuromuscular junction is coinnervated by approximately ten highly intermingled axons (versus one in adults). Extensive die off of terminal branches occurs during the first several postnatal days, leading to much sparser arbors that still span the same territory. Despite the extensive pruning, total axoplasm per neuron increases as axons elongate, thicken, and add more synaptic release sites on their remaining targets. Motor axons therefore initially establish weak connections with nearly all available postsynaptic targets but, beginning at birth, massively redistribute synaptic resources, concentrating many more synaptic sites on many fewer muscle fibers. Analogous changes in connectivity may occur in the CNS. National Institutes of Health (U.S.) Gatsby Charitable Trust Harvard University. Center for Brain Science 2014-11-13T19:27:57Z 2014-11-13T19:27:57Z 2012-06 2012-04 Article http://purl.org/eprint/type/JournalArticle 08966273 1097-4199 http://hdl.handle.net/1721.1/91553 Tapia, Juan C., John D. Wylie, Narayanan Kasthuri, Kenneth J. Hayworth, Richard Schalek, Daniel R. Berger, Cristina Guatimosim, H. Sebastian Seung, and Jeff W. Lichtman. “Pervasive Synaptic Branch Removal in the Mammalian Neuromuscular System at Birth.” Neuron 74, no. 5 (June 2012): 816–829. © 2012 Elsevier Inc. en_US http://dx.doi.org/10.1016/j.neuron.2012.04.017 Neuron Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier |
| spellingShingle | Tapia, Juan C. Wylie, John D. Kasthuri, Narayanan Hayworth, Kenneth J. Schalek, Richard Berger, Daniel R. Guatimosim, Cristina Seung, H. Sebastian Lichtman, Jeff W. Pervasive Synaptic Branch Removal in the Mammalian Neuromuscular System at Birth |
| title | Pervasive Synaptic Branch Removal in the Mammalian Neuromuscular System at Birth |
| title_full | Pervasive Synaptic Branch Removal in the Mammalian Neuromuscular System at Birth |
| title_fullStr | Pervasive Synaptic Branch Removal in the Mammalian Neuromuscular System at Birth |
| title_full_unstemmed | Pervasive Synaptic Branch Removal in the Mammalian Neuromuscular System at Birth |
| title_short | Pervasive Synaptic Branch Removal in the Mammalian Neuromuscular System at Birth |
| title_sort | pervasive synaptic branch removal in the mammalian neuromuscular system at birth |
| url | http://hdl.handle.net/1721.1/91553 |
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