Expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism
The metabolic engineer's toolbox, comprising stable isotope tracers, flux estimation and analysis, pathway identification, and pathway kinetics and regulation, among other techniques, has long been used to elucidate and quantify pathways primarily in the context of engineering microbes for prod...
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2014
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| Online Access: | http://hdl.handle.net/1721.1/91560 https://orcid.org/0000-0002-5410-6543 https://orcid.org/0000-0001-6909-4568 |
| _version_ | 1826199197084286976 |
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| author | Fendt, Sarah-Maria Stephanopoulos, Gregory Keibler, Mark Andrew |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Fendt, Sarah-Maria Stephanopoulos, Gregory Keibler, Mark Andrew |
| author_sort | Fendt, Sarah-Maria |
| collection | MIT |
| description | The metabolic engineer's toolbox, comprising stable isotope tracers, flux estimation and analysis, pathway identification, and pathway kinetics and regulation, among other techniques, has long been used to elucidate and quantify pathways primarily in the context of engineering microbes for producing small molecules of interest. Recently, these tools are increasingly finding use in cancer biology due to their unparalleled capacity for quantifying intracellular metabolism of mammalian cells. Here, we review basic concepts that are used to derive useful insights about the metabolism of tumor cells, along with a number of illustrative examples highlighting the fundamental contributions of these methods to elucidating cancer cell metabolism. This area presents unique opportunities for metabolic engineering to expand its portfolio of applications into the realm of cancer biology and help develop new cancer therapies based on a new class of metabolically derived targets. |
| first_indexed | 2024-09-23T11:16:16Z |
| format | Article |
| id | mit-1721.1/91560 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:16:16Z |
| publishDate | 2014 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/915602022-10-01T02:28:15Z Expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism Fendt, Sarah-Maria Stephanopoulos, Gregory Keibler, Mark Andrew Massachusetts Institute of Technology. Department of Chemical Engineering Keibler, Mark Andrew Fendt, Sarah-Maria Stephanopoulos, Gregory The metabolic engineer's toolbox, comprising stable isotope tracers, flux estimation and analysis, pathway identification, and pathway kinetics and regulation, among other techniques, has long been used to elucidate and quantify pathways primarily in the context of engineering microbes for producing small molecules of interest. Recently, these tools are increasingly finding use in cancer biology due to their unparalleled capacity for quantifying intracellular metabolism of mammalian cells. Here, we review basic concepts that are used to derive useful insights about the metabolism of tumor cells, along with a number of illustrative examples highlighting the fundamental contributions of these methods to elucidating cancer cell metabolism. This area presents unique opportunities for metabolic engineering to expand its portfolio of applications into the realm of cancer biology and help develop new cancer therapies based on a new class of metabolically derived targets. German Science Foundation National Institutes of Health (U.S.) (Grant 1R01 DK075850-01) National Institutes of Health (U.S.) (Grant R01 CA160458) 2014-11-13T19:52:35Z 2014-11-13T19:52:35Z 2012-10 2012-08 Article http://purl.org/eprint/type/JournalArticle 87567938 1520-6033 http://hdl.handle.net/1721.1/91560 Keibler, Mark A., Sarah-Maria Fendt, and Gregory Stephanopoulos. “Expanding the Concepts and Tools of Metabolic Engineering to Elucidate Cancer Metabolism.” Biotechnology Progress 28, no. 6 (October 18, 2012): 1409–1418. https://orcid.org/0000-0002-5410-6543 https://orcid.org/0000-0001-6909-4568 en_US http://dx.doi.org/10.1002/btpr.1629 Biotechnology Progress Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Fendt, Sarah-Maria Stephanopoulos, Gregory Keibler, Mark Andrew Expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism |
| title | Expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism |
| title_full | Expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism |
| title_fullStr | Expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism |
| title_full_unstemmed | Expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism |
| title_short | Expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism |
| title_sort | expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism |
| url | http://hdl.handle.net/1721.1/91560 https://orcid.org/0000-0002-5410-6543 https://orcid.org/0000-0001-6909-4568 |
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