In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes
Single-walled carbon nanotubes are particularly attractive for biomedical applications, because they exhibit a fluorescent signal in a spectral region where there is minimal interference from biological media. Although single-walled carbon nanotubes have been used as highly sensitive detectors for v...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/91579 https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0002-5166-1410 |
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| author | Iverson, Nicole M. Barone, Paul W. Shandell, Mia Trudel, Laura J. Sen, Selda Sen, Fatih Ivanov, Vsevolod Atolia, Esha Farias, Edgardo McNicholas, Thomas P. Reuel, Nigel Parry, Nicola M. A. Wogan, Gerald N. Strano, Michael S. |
| author2 | Massachusetts Institute of Technology. Center for Biomedical Innovation |
| author_facet | Massachusetts Institute of Technology. Center for Biomedical Innovation Iverson, Nicole M. Barone, Paul W. Shandell, Mia Trudel, Laura J. Sen, Selda Sen, Fatih Ivanov, Vsevolod Atolia, Esha Farias, Edgardo McNicholas, Thomas P. Reuel, Nigel Parry, Nicola M. A. Wogan, Gerald N. Strano, Michael S. |
| author_sort | Iverson, Nicole M. |
| collection | MIT |
| description | Single-walled carbon nanotubes are particularly attractive for biomedical applications, because they exhibit a fluorescent signal in a spectral region where there is minimal interference from biological media. Although single-walled carbon nanotubes have been used as highly sensitive detectors for various compounds, their use as in vivo biomarkers requires the simultaneous optimization of various parameters, including biocompatibility, molecular recognition, high fluorescence quantum efficiency and signal transduction. Here we show that a polyethylene glycol ligated copolymer stabilizes near-infrared-fluorescent single-walled carbon nanotubes sensors in solution, enabling intravenous injection into mice and the selective detection of local nitric oxide concentration with a detection limit of 1 µM. The half-life for liver retention is 4 h, with sensors clearing the lungs within 2 h after injection, thus avoiding a dominant route of in vivo nanotoxicology. After localization within the liver, it is possible to follow the transient inflammation using nitric oxide as a marker and signalling molecule. To this end, we also report a spatial-spectral imaging algorithm to deconvolute fluorescence intensity and spatial information from measurements. Finally, we demonstrate that alginate-encapsulated single-walled carbon nanotubes can function as implantable inflammation sensors for nitric oxide detection, with no intrinsic immune reactivity or other adverse response for more than 400 days. |
| first_indexed | 2024-09-23T09:51:18Z |
| format | Article |
| id | mit-1721.1/91579 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:51:18Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/915792022-09-30T17:13:16Z In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes Iverson, Nicole M. Barone, Paul W. Shandell, Mia Trudel, Laura J. Sen, Selda Sen, Fatih Ivanov, Vsevolod Atolia, Esha Farias, Edgardo McNicholas, Thomas P. Reuel, Nigel Parry, Nicola M. A. Wogan, Gerald N. Strano, Michael S. Massachusetts Institute of Technology. Center for Biomedical Innovation Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Division of Comparative Medicine Iverson, Nicole M. Barone, Paul W. Shandell, Mia Sen, Selda Sen, Fatih McNicholas, Thomas P. Reuel, Nigel Strano, Michael S. Trudel, Laura J. Ivanov, Vsevolod Atolia, Esha Farias, Edgardo Wogan, Gerald N. Parry, Nicola M. A. Single-walled carbon nanotubes are particularly attractive for biomedical applications, because they exhibit a fluorescent signal in a spectral region where there is minimal interference from biological media. Although single-walled carbon nanotubes have been used as highly sensitive detectors for various compounds, their use as in vivo biomarkers requires the simultaneous optimization of various parameters, including biocompatibility, molecular recognition, high fluorescence quantum efficiency and signal transduction. Here we show that a polyethylene glycol ligated copolymer stabilizes near-infrared-fluorescent single-walled carbon nanotubes sensors in solution, enabling intravenous injection into mice and the selective detection of local nitric oxide concentration with a detection limit of 1 µM. The half-life for liver retention is 4 h, with sensors clearing the lungs within 2 h after injection, thus avoiding a dominant route of in vivo nanotoxicology. After localization within the liver, it is possible to follow the transient inflammation using nitric oxide as a marker and signalling molecule. To this end, we also report a spatial-spectral imaging algorithm to deconvolute fluorescence intensity and spatial information from measurements. Finally, we demonstrate that alginate-encapsulated single-walled carbon nanotubes can function as implantable inflammation sensors for nitric oxide detection, with no intrinsic immune reactivity or other adverse response for more than 400 days. National Institutes of Health (U.S.) (T32 Training Grant in Environmental Toxicology ES007020) National Cancer Institute (U.S.) (Grant P01 CA26731) National Institute of Environmental Health Sciences (Grant P30 ES002109) Arnold and Mabel Beckman Foundation (Young Investigator Award) National Science Foundation (U.S.). Presidential Early Career Award for Scientists and Engineers Scientific and Technological Research Council of Turkey (TUBITAK 2211 Research Fellowship Programme) Scientific and Technological Research Council of Turkey (TUBITAK 2214 Research Fellowship Programme) Middle East Technical University. Faculty Development Programme Sanofi Aventis (Firm) (Biomedical Innovation Grant) 2014-11-14T18:37:30Z 2014-11-14T18:37:30Z 2013-11 2013-02 Article http://purl.org/eprint/type/JournalArticle 1748-3387 1748-3395 http://hdl.handle.net/1721.1/91579 Iverson, Nicole M., Paul W. Barone, Mia Shandell, Laura J. Trudel, Selda Sen, Fatih Sen, Vsevolod Ivanov, et al. “In Vivo Biosensing via Tissue-Localizable Near-Infrared-Fluorescent Single-Walled Carbon Nanotubes.” Nature Nanotechnology 8, no. 11 (November 3, 2013): 873–880. https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0002-5166-1410 en_US http://dx.doi.org/10.1038/nnano.2013.222 Nature Nanotechnology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Iverson, Nicole M. Barone, Paul W. Shandell, Mia Trudel, Laura J. Sen, Selda Sen, Fatih Ivanov, Vsevolod Atolia, Esha Farias, Edgardo McNicholas, Thomas P. Reuel, Nigel Parry, Nicola M. A. Wogan, Gerald N. Strano, Michael S. In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes |
| title | In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes |
| title_full | In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes |
| title_fullStr | In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes |
| title_full_unstemmed | In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes |
| title_short | In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes |
| title_sort | in vivo biosensing via tissue localizable near infrared fluorescent single walled carbon nanotubes |
| url | http://hdl.handle.net/1721.1/91579 https://orcid.org/0000-0003-0771-9889 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0002-5166-1410 |
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