Engineered yeast for enhanced CO2 mineralization
In this work, a biologically catalysed CO2 mineralization process for the capture of CO2 from point sources was designed, constructed at a laboratory scale, and, using standard chemical process scale-up protocols, was modelled and evaluated at an industrial scale. A yeast display system in Saccharom...
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| Format: | Article |
| Language: | en_US |
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Royal Society of Chemistry
2014
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| Online Access: | http://hdl.handle.net/1721.1/91613 https://orcid.org/0000-0001-9353-7453 |
| _version_ | 1811096274806505472 |
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| author | Barbero, Roberto Juan Carnelli, Lino Simon, Anna Kao, Albert Monforte, Alessandra d'Arminio Riccò, Moreno Bianchi, Daniele Belcher, Angela M |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Barbero, Roberto Juan Carnelli, Lino Simon, Anna Kao, Albert Monforte, Alessandra d'Arminio Riccò, Moreno Bianchi, Daniele Belcher, Angela M |
| author_sort | Barbero, Roberto Juan |
| collection | MIT |
| description | In this work, a biologically catalysed CO2 mineralization process for the capture of CO2 from point sources was designed, constructed at a laboratory scale, and, using standard chemical process scale-up protocols, was modelled and evaluated at an industrial scale. A yeast display system in Saccharomyces cerevisae was used to screen several carbonic anhydrase isoforms and mineralization peptides for their impact on CO2 hydration, CaCO3 mineralization, and particle settling rate. Enhanced rates for each of these steps in the CaCO3 mineralization process were confirmed using quantitative techniques in lab-scale measurements. The effect of these enhanced rates on the CO2 capture cost in an industrial scale CO2 mineralization process using coal fly ash as the CaO source was evaluated. The model predicts a process using bCA2-yeast and fly ash is [similar]10% more cost effective per tonne of CO2 captured than a process with no biological molecules, a savings not realized by wild-type yeast and high-temperature stable recombinant CA2 alone or in combination. The levelized cost of electricity for a power plant using this process was calculated and scenarios in which this process compares favourably to CO2 capture by MEA absorption process are presented. |
| first_indexed | 2024-09-23T16:41:17Z |
| format | Article |
| id | mit-1721.1/91613 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:41:17Z |
| publishDate | 2014 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/916132022-10-03T07:34:52Z Engineered yeast for enhanced CO2 mineralization Barbero, Roberto Juan Carnelli, Lino Simon, Anna Kao, Albert Monforte, Alessandra d'Arminio Riccò, Moreno Bianchi, Daniele Belcher, Angela M Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Koch Institute for Integrative Cancer Research at MIT Barbero, Roberto Juan Belcher, Angela M. In this work, a biologically catalysed CO2 mineralization process for the capture of CO2 from point sources was designed, constructed at a laboratory scale, and, using standard chemical process scale-up protocols, was modelled and evaluated at an industrial scale. A yeast display system in Saccharomyces cerevisae was used to screen several carbonic anhydrase isoforms and mineralization peptides for their impact on CO2 hydration, CaCO3 mineralization, and particle settling rate. Enhanced rates for each of these steps in the CaCO3 mineralization process were confirmed using quantitative techniques in lab-scale measurements. The effect of these enhanced rates on the CO2 capture cost in an industrial scale CO2 mineralization process using coal fly ash as the CaO source was evaluated. The model predicts a process using bCA2-yeast and fly ash is [similar]10% more cost effective per tonne of CO2 captured than a process with no biological molecules, a savings not realized by wild-type yeast and high-temperature stable recombinant CA2 alone or in combination. The levelized cost of electricity for a power plant using this process was calculated and scenarios in which this process compares favourably to CO2 capture by MEA absorption process are presented. MIT Energy Initiative Eni S.p.A. (Firm) National Institutes of Health (U.S.) (NIH Biotechnology Training Program) Thomas and Stacey Siebel Foundation 2014-11-19T19:22:54Z 2014-11-19T19:22:54Z 2013 2012-03 Article http://purl.org/eprint/type/JournalArticle 1754-5692 1754-5706 http://hdl.handle.net/1721.1/91613 Barbero, Roberto, Lino Carnelli, Anna Simon, Albert Kao, Alessandra d’Arminio Monforte, Moreno Riccò, Daniele Bianchi, and Angela Belcher. “Engineered Yeast for Enhanced CO2 Mineralization.” Energy & Environmental Science 6, no. 2 (2013): 660. https://orcid.org/0000-0001-9353-7453 en_US http://dx.doi.org/10.1039/c2ee24060b Energy & Environmental Science Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Royal Society of Chemistry PMC |
| spellingShingle | Barbero, Roberto Juan Carnelli, Lino Simon, Anna Kao, Albert Monforte, Alessandra d'Arminio Riccò, Moreno Bianchi, Daniele Belcher, Angela M Engineered yeast for enhanced CO2 mineralization |
| title | Engineered yeast for enhanced CO2 mineralization |
| title_full | Engineered yeast for enhanced CO2 mineralization |
| title_fullStr | Engineered yeast for enhanced CO2 mineralization |
| title_full_unstemmed | Engineered yeast for enhanced CO2 mineralization |
| title_short | Engineered yeast for enhanced CO2 mineralization |
| title_sort | engineered yeast for enhanced co2 mineralization |
| url | http://hdl.handle.net/1721.1/91613 https://orcid.org/0000-0001-9353-7453 |
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