Clustered Dynamics of Inhibitory Synapses and Dendritic Spines in the Adult Neocortex
A key feature of the mammalian brain is its capacity to adapt in response to experience, in part by remodeling of synaptic connections between neurons. Excitatory synapse rearrangements have been monitored in vivo by observation of dendritic spine dynamics, but lack of a vital marker for inhibitory...
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| Language: | en_US |
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Elsevier B.V.
2014
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| Online Access: | http://hdl.handle.net/1721.1/91709 https://orcid.org/0000-0002-6791-286X https://orcid.org/0000-0002-1710-0767 https://orcid.org/0000-0003-4698-6488 |
| _version_ | 1826197941756362752 |
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| author | Chen, Jerry L. Villa, Katherine Leigh Cha, Jae Won So, Peter T. C. Kubota, Yoshiyuki Nedivi, Elly |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Chen, Jerry L. Villa, Katherine Leigh Cha, Jae Won So, Peter T. C. Kubota, Yoshiyuki Nedivi, Elly |
| author_sort | Chen, Jerry L. |
| collection | MIT |
| description | A key feature of the mammalian brain is its capacity to adapt in response to experience, in part by remodeling of synaptic connections between neurons. Excitatory synapse rearrangements have been monitored in vivo by observation of dendritic spine dynamics, but lack of a vital marker for inhibitory synapses has precluded their observation. Here, we simultaneously monitor in vivo inhibitory synapse and dendritic spine dynamics across the entire dendritic arbor of pyramidal neurons in the adult mammalian cortex using large-volume, high-resolution dual-color two-photon microscopy. We find that inhibitory synapses on dendritic shafts and spines differ in their distribution across the arbor and in their remodeling kinetics during normal and altered sensory experience. Further, we find inhibitory synapse and dendritic spine remodeling to be spatially clustered and that clustering is influenced by sensory input. Our findings provide in vivo evidence for local coordination of inhibitory and excitatory synaptic rearrangements. |
| first_indexed | 2024-09-23T10:56:08Z |
| format | Article |
| id | mit-1721.1/91709 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:56:08Z |
| publishDate | 2014 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | mit-1721.1/917092022-09-27T15:58:56Z Clustered Dynamics of Inhibitory Synapses and Dendritic Spines in the Adult Neocortex Chen, Jerry L. Villa, Katherine Leigh Cha, Jae Won So, Peter T. C. Kubota, Yoshiyuki Nedivi, Elly Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Mechanical Engineering Picower Institute for Learning and Memory Chen, Jerry L. Villa, Katherine Leigh Cha, Jae Won So, Peter T. C. Nedivi, Elly A key feature of the mammalian brain is its capacity to adapt in response to experience, in part by remodeling of synaptic connections between neurons. Excitatory synapse rearrangements have been monitored in vivo by observation of dendritic spine dynamics, but lack of a vital marker for inhibitory synapses has precluded their observation. Here, we simultaneously monitor in vivo inhibitory synapse and dendritic spine dynamics across the entire dendritic arbor of pyramidal neurons in the adult mammalian cortex using large-volume, high-resolution dual-color two-photon microscopy. We find that inhibitory synapses on dendritic shafts and spines differ in their distribution across the arbor and in their remodeling kinetics during normal and altered sensory experience. Further, we find inhibitory synapse and dendritic spine remodeling to be spatially clustered and that clustering is influenced by sensory input. Our findings provide in vivo evidence for local coordination of inhibitory and excitatory synaptic rearrangements. National Eye Institute (RO1 EY017656) National Eye Institute (RO1 EY011894) Singapore-MIT Alliance (Singapore-MIT Alliance-2) Singapore-MIT Alliance for Research and Technology Japan. Ministry of Education, Culture, Sports, Science and Technology (Grant-in-aid for Scientific Research on Innovative Areas: Neural creativity for communication (No.4103) (22120518)) 2014-11-24T17:01:53Z 2014-11-24T17:01:53Z 2012-04 Article http://purl.org/eprint/type/JournalArticle 08966273 http://hdl.handle.net/1721.1/91709 Chen, Jerry L., Katherine L. Villa, Jae Won Cha, Peter T.C. So, Yoshiyuki Kubota, and Elly Nedivi. “Clustered Dynamics of Inhibitory Synapses and Dendritic Spines in the Adult Neocortex.” Neuron 74, no. 2 (April 2012): 361–373. © 2012 Elsevier Inc. https://orcid.org/0000-0002-6791-286X https://orcid.org/0000-0002-1710-0767 https://orcid.org/0000-0003-4698-6488 en_US http://dx.doi.org/10.1016/j.neuron.2012.02.030 Neuron Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier B.V. Elsevier |
| spellingShingle | Chen, Jerry L. Villa, Katherine Leigh Cha, Jae Won So, Peter T. C. Kubota, Yoshiyuki Nedivi, Elly Clustered Dynamics of Inhibitory Synapses and Dendritic Spines in the Adult Neocortex |
| title | Clustered Dynamics of Inhibitory Synapses and Dendritic Spines in the Adult Neocortex |
| title_full | Clustered Dynamics of Inhibitory Synapses and Dendritic Spines in the Adult Neocortex |
| title_fullStr | Clustered Dynamics of Inhibitory Synapses and Dendritic Spines in the Adult Neocortex |
| title_full_unstemmed | Clustered Dynamics of Inhibitory Synapses and Dendritic Spines in the Adult Neocortex |
| title_short | Clustered Dynamics of Inhibitory Synapses and Dendritic Spines in the Adult Neocortex |
| title_sort | clustered dynamics of inhibitory synapses and dendritic spines in the adult neocortex |
| url | http://hdl.handle.net/1721.1/91709 https://orcid.org/0000-0002-6791-286X https://orcid.org/0000-0002-1710-0767 https://orcid.org/0000-0003-4698-6488 |
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