Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice
The ability to chronically monitor neuronal activity in the living brain is essential for understanding the organization and function of the nervous system. The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuro...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/91895 https://orcid.org/0000-0002-8021-277X https://orcid.org/0000-0001-9473-2402 |
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| author | Chen, Qian Cichon, Joseph Wang, Wenting Qiu, Li Lee, Seok-Jin R. Campbell, Nolan R. DeStefino, Nicholas Fu, Zhanyan Yasuda, Ryohei Looger, Loren L. Arenkiel, Benjamin R. Gan, Wen-Biao Feng, Guoping |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Chen, Qian Cichon, Joseph Wang, Wenting Qiu, Li Lee, Seok-Jin R. Campbell, Nolan R. DeStefino, Nicholas Fu, Zhanyan Yasuda, Ryohei Looger, Loren L. Arenkiel, Benjamin R. Gan, Wen-Biao Feng, Guoping |
| author_sort | Chen, Qian |
| collection | MIT |
| description | The ability to chronically monitor neuronal activity in the living brain is essential for understanding the organization and function of the nervous system. The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, processes, and synapses that are triggered by neuronal activities. Here we report the generation and characterization of transgenic mice that express improved GCaMPs in various neuronal subpopulations under the control of the Thy1 promoter. In vitro and in vivo studies show that calcium transients induced by spontaneous and stimulus-evoked neuronal activities can be readily detected at the level of individual cells and synapses in acute brain slices, as well as chronically in awake, behaving animals. These GCaMP transgenic mice allow investigation of activity patterns in defined neuronal populations in the living brain and will greatly facilitate dissecting complex structural and functional relationships of neural networks. |
| first_indexed | 2024-09-23T11:34:59Z |
| format | Article |
| id | mit-1721.1/91895 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:34:59Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/918952022-10-01T04:35:04Z Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice Chen, Qian Cichon, Joseph Wang, Wenting Qiu, Li Lee, Seok-Jin R. Campbell, Nolan R. DeStefino, Nicholas Fu, Zhanyan Yasuda, Ryohei Looger, Loren L. Arenkiel, Benjamin R. Gan, Wen-Biao Feng, Guoping Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Picower Institute for Learning and Memory Chen, Qian Wang, Wenting Campbell, Nolan R. Goard, Michael Fu, Zhanyan Feng, Guoping The ability to chronically monitor neuronal activity in the living brain is essential for understanding the organization and function of the nervous system. The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, processes, and synapses that are triggered by neuronal activities. Here we report the generation and characterization of transgenic mice that express improved GCaMPs in various neuronal subpopulations under the control of the Thy1 promoter. In vitro and in vivo studies show that calcium transients induced by spontaneous and stimulus-evoked neuronal activities can be readily detected at the level of individual cells and synapses in acute brain slices, as well as chronically in awake, behaving animals. These GCaMP transgenic mice allow investigation of activity patterns in defined neuronal populations in the living brain and will greatly facilitate dissecting complex structural and functional relationships of neural networks. Poitras Center for Affective Disorders Research 2014-11-24T20:42:38Z 2014-11-24T20:42:38Z 2012-10 Article http://purl.org/eprint/type/JournalArticle 08966273 1097-4199 http://hdl.handle.net/1721.1/91895 Chen, Qian, Joseph Cichon, Wenting Wang, Li Qiu, Seok-Jin R. Lee, Nolan R. Campbell, Nicholas DeStefino, et al. “Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice.” Neuron 76, no. 2 (October 2012): 297–308. © 2012 Elsevier Inc. https://orcid.org/0000-0002-8021-277X https://orcid.org/0000-0001-9473-2402 en_US http://dx.doi.org/10.1016/j.neuron.2012.07.011 Neuron Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier |
| spellingShingle | Chen, Qian Cichon, Joseph Wang, Wenting Qiu, Li Lee, Seok-Jin R. Campbell, Nolan R. DeStefino, Nicholas Fu, Zhanyan Yasuda, Ryohei Looger, Loren L. Arenkiel, Benjamin R. Gan, Wen-Biao Feng, Guoping Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice |
| title | Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice |
| title_full | Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice |
| title_fullStr | Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice |
| title_full_unstemmed | Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice |
| title_short | Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice |
| title_sort | imaging neural activity using thy1 gcamp transgenic mice |
| url | http://hdl.handle.net/1721.1/91895 https://orcid.org/0000-0002-8021-277X https://orcid.org/0000-0001-9473-2402 |
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