Replication-Coupled Chromatin Assembly Generates a Neuronal Bilateral Asymmetry in C. elegans
Although replication-coupled chromatin assembly is known to be important for the maintenance of patterns of gene expression through sequential cell divisions, the role of replication-coupled chromatin assembly in controlling cell differentiation during animal development remains largely unexplored....
| Main Authors: | , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/92340 https://orcid.org/0000-0002-9964-9613 |
| _version_ | 1826201631735152640 |
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| author | Nakano, Shunji Stillman, Bruce Horvitz, Howard Robert |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Nakano, Shunji Stillman, Bruce Horvitz, Howard Robert |
| author_sort | Nakano, Shunji |
| collection | MIT |
| description | Although replication-coupled chromatin assembly is known to be important for the maintenance of patterns of gene expression through sequential cell divisions, the role of replication-coupled chromatin assembly in controlling cell differentiation during animal development remains largely unexplored. Here we report that the CAF-1 protein complex, an evolutionarily conserved histone chaperone that deposits histone H3-H4 proteins onto replicating DNA, is required to generate a bilateral asymmetry in the C. elegans nervous system. A mutation in 1 of 24 C. elegans histone H3 genes specifically eliminates this aspect of neuronal asymmetry by causing a defect in the formation of a histone H3-H4 tetramer and the consequent inhibition of CAF-1-mediated nucleosome formation. Our results reveal that replication-coupled nucleosome assembly is necessary to generate a bilateral asymmetry in C. elegans neuroanatomy and suggest that left-right asymmetric epigenetic regulation can establish bilateral asymmetry in the nervous system. |
| first_indexed | 2024-09-23T11:54:41Z |
| format | Article |
| id | mit-1721.1/92340 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:54:41Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/923402022-10-01T06:52:30Z Replication-Coupled Chromatin Assembly Generates a Neuronal Bilateral Asymmetry in C. elegans Nakano, Shunji Stillman, Bruce Horvitz, Howard Robert Massachusetts Institute of Technology. Department of Biology Horvitz, H. Robert Nakano, Shunji Although replication-coupled chromatin assembly is known to be important for the maintenance of patterns of gene expression through sequential cell divisions, the role of replication-coupled chromatin assembly in controlling cell differentiation during animal development remains largely unexplored. Here we report that the CAF-1 protein complex, an evolutionarily conserved histone chaperone that deposits histone H3-H4 proteins onto replicating DNA, is required to generate a bilateral asymmetry in the C. elegans nervous system. A mutation in 1 of 24 C. elegans histone H3 genes specifically eliminates this aspect of neuronal asymmetry by causing a defect in the formation of a histone H3-H4 tetramer and the consequent inhibition of CAF-1-mediated nucleosome formation. Our results reveal that replication-coupled nucleosome assembly is necessary to generate a bilateral asymmetry in C. elegans neuroanatomy and suggest that left-right asymmetric epigenetic regulation can establish bilateral asymmetry in the nervous system. Howard Hughes Medical Institute National Cancer Institute (U.S.) (Grant CA13106) 2014-12-16T19:32:25Z 2014-12-16T19:32:25Z 2011-12 2011-09 Article http://purl.org/eprint/type/JournalArticle 00928674 1097-4172 http://hdl.handle.net/1721.1/92340 Nakano, Shunji, Bruce Stillman, and H. Robert Horvitz. “Replication-Coupled Chromatin Assembly Generates a Neuronal Bilateral Asymmetry in C. Elegans.” Cell 147, no. 7 (December 2011): 1525–1536. © 2011 Elsevier Inc. https://orcid.org/0000-0002-9964-9613 en_US http://dx.doi.org/10.1016/j.cell.2011.11.053 Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier |
| spellingShingle | Nakano, Shunji Stillman, Bruce Horvitz, Howard Robert Replication-Coupled Chromatin Assembly Generates a Neuronal Bilateral Asymmetry in C. elegans |
| title | Replication-Coupled Chromatin Assembly Generates a Neuronal Bilateral Asymmetry in C. elegans |
| title_full | Replication-Coupled Chromatin Assembly Generates a Neuronal Bilateral Asymmetry in C. elegans |
| title_fullStr | Replication-Coupled Chromatin Assembly Generates a Neuronal Bilateral Asymmetry in C. elegans |
| title_full_unstemmed | Replication-Coupled Chromatin Assembly Generates a Neuronal Bilateral Asymmetry in C. elegans |
| title_short | Replication-Coupled Chromatin Assembly Generates a Neuronal Bilateral Asymmetry in C. elegans |
| title_sort | replication coupled chromatin assembly generates a neuronal bilateral asymmetry in c elegans |
| url | http://hdl.handle.net/1721.1/92340 https://orcid.org/0000-0002-9964-9613 |
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