Substrate Viscosity Enhances Correlation in Epithelial Sheet Movement
The movement of the epithelium plays vital roles in the development and renewal of complex tissues, from the separation of tissues in the early embryo, to turnover in the homeostasis of the gastrointestinal mucosa. Yet, despite its importance, a clear interpretation of the mechanism for collective m...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/92342 https://orcid.org/0000-0002-7232-304X |
| _version_ | 1811077788933816320 |
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| author | Murrell, Michael Kamm, Roger Dale Matsudaira, Paul T. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Murrell, Michael Kamm, Roger Dale Matsudaira, Paul T. |
| author_sort | Murrell, Michael |
| collection | MIT |
| description | The movement of the epithelium plays vital roles in the development and renewal of complex tissues, from the separation of tissues in the early embryo, to turnover in the homeostasis of the gastrointestinal mucosa. Yet, despite its importance, a clear interpretation of the mechanism for collective motion in epithelial sheets remains elusive. This interpretation is prohibited by the lack of understanding of the relationship between motion and cell-cell contact, and their mediation by the mechanical properties of the underlying substrate. To better mimic physiological substrates that have inherent viscosity, we probe this relationship using polydimethylsiloxane, a substrate whose mechanical properties can be tuned from predominantly elastic to viscous by altering its cross-linking content. We therefore characterize the comparative spatiotemporal correlations in cell velocity during the movement of an epithelial monolayer as a function of the viscoelasticity of the substrate. Our results show that high correlation in cell velocity is achieved when the substrate G″(ω) is ∼0.4 × G′(ω). This correlation is driven by a balance between cell-cell contact and the adhesion and contraction of the extracellular matrix. For G″(ω) > G′(ω), this balance shifts, and contraction of the tissue drives the substrate to flow, further elevating the correlation in movement. |
| first_indexed | 2024-09-23T10:48:24Z |
| format | Article |
| id | mit-1721.1/92342 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:48:24Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/923422022-09-30T23:08:55Z Substrate Viscosity Enhances Correlation in Epithelial Sheet Movement Murrell, Michael Kamm, Roger Dale Matsudaira, Paul T. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Mechanical Engineering Kamm, Roger Dale Matsudaira, Paul T. Murrell, Michael The movement of the epithelium plays vital roles in the development and renewal of complex tissues, from the separation of tissues in the early embryo, to turnover in the homeostasis of the gastrointestinal mucosa. Yet, despite its importance, a clear interpretation of the mechanism for collective motion in epithelial sheets remains elusive. This interpretation is prohibited by the lack of understanding of the relationship between motion and cell-cell contact, and their mediation by the mechanical properties of the underlying substrate. To better mimic physiological substrates that have inherent viscosity, we probe this relationship using polydimethylsiloxane, a substrate whose mechanical properties can be tuned from predominantly elastic to viscous by altering its cross-linking content. We therefore characterize the comparative spatiotemporal correlations in cell velocity during the movement of an epithelial monolayer as a function of the viscoelasticity of the substrate. Our results show that high correlation in cell velocity is achieved when the substrate G″(ω) is ∼0.4 × G′(ω). This correlation is driven by a balance between cell-cell contact and the adhesion and contraction of the extracellular matrix. For G″(ω) > G′(ω), this balance shifts, and contraction of the tissue drives the substrate to flow, further elevating the correlation in movement. National Institutes of Health (U.S.). Biotechnology Training Grant Singapore-MIT Alliance for Research and Technology Massachusetts Institute of Technology. Presidential Fellowship 2014-12-16T19:59:32Z 2014-12-16T19:59:32Z 2011-07 2010-09 Article http://purl.org/eprint/type/JournalArticle 00063495 1542-0086 http://hdl.handle.net/1721.1/92342 Murrell, Michael, Roger Kamm, and Paul Matsudaira. “Substrate Viscosity Enhances Correlation in Epithelial Sheet Movement.” Biophysical Journal 101, no. 2 (July 2011): 297–306. © 2011 Biophysical Society https://orcid.org/0000-0002-7232-304X en_US http://dx.doi.org/10.1016/j.bpj.2011.05.048 Biophysical Journal Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier |
| spellingShingle | Murrell, Michael Kamm, Roger Dale Matsudaira, Paul T. Substrate Viscosity Enhances Correlation in Epithelial Sheet Movement |
| title | Substrate Viscosity Enhances Correlation in Epithelial Sheet Movement |
| title_full | Substrate Viscosity Enhances Correlation in Epithelial Sheet Movement |
| title_fullStr | Substrate Viscosity Enhances Correlation in Epithelial Sheet Movement |
| title_full_unstemmed | Substrate Viscosity Enhances Correlation in Epithelial Sheet Movement |
| title_short | Substrate Viscosity Enhances Correlation in Epithelial Sheet Movement |
| title_sort | substrate viscosity enhances correlation in epithelial sheet movement |
| url | http://hdl.handle.net/1721.1/92342 https://orcid.org/0000-0002-7232-304X |
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