Independent optical excitation of distinct neural populations
Optogenetic tools enable examination of how specific cell types contribute to brain circuit functions. A long-standing question is whether it is possible to independently activate two distinct neural populations in mammalian brain tissue. Such a capability would enable the study of how different syn...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/92352 https://orcid.org/0000-0003-2268-0863 https://orcid.org/0000-0002-0419-3351 |
| _version_ | 1826190292997373952 |
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| author | Klapoetke, Nathan Cao Murata, Yasunobu Kim, Sung Soo Pulver, Stefan R. Birdsey-Benson, Amanda Cho, Yong Ku Morimoto, Tania K. Chuong, Amy S. Carpenter, Eric J. Tian, Zhijian Wang, Jun Xie, Yinlong Yan, Zhixiang Zhang, Yong Chow, Brian Y. Surek, Barbara Melkonian, Michael Jayaraman, Vivek Wong, Gane Ka-Shu Constantine-Paton, Martha Boyden, Edward |
| author2 | Massachusetts Institute of Technology. Synthetic Neurobiology Group |
| author_facet | Massachusetts Institute of Technology. Synthetic Neurobiology Group Klapoetke, Nathan Cao Murata, Yasunobu Kim, Sung Soo Pulver, Stefan R. Birdsey-Benson, Amanda Cho, Yong Ku Morimoto, Tania K. Chuong, Amy S. Carpenter, Eric J. Tian, Zhijian Wang, Jun Xie, Yinlong Yan, Zhixiang Zhang, Yong Chow, Brian Y. Surek, Barbara Melkonian, Michael Jayaraman, Vivek Wong, Gane Ka-Shu Constantine-Paton, Martha Boyden, Edward |
| author_sort | Klapoetke, Nathan Cao |
| collection | MIT |
| description | Optogenetic tools enable examination of how specific cell types contribute to brain circuit functions. A long-standing question is whether it is possible to independently activate two distinct neural populations in mammalian brain tissue. Such a capability would enable the study of how different synapses or pathways interact to encode information in the brain. Here we describe two channelrhodopsins, Chronos and Chrimson, discovered through sequencing and physiological characterization of opsins from over 100 species of alga. Chrimson's excitation spectrum is red shifted by 45 nm relative to previous channelrhodopsins and can enable experiments in which red light is preferred. We show minimal visual system–mediated behavioral interference when using the variant CsChrimson in neurobehavioral studies in Drosophila melanogaster. Chronos has faster kinetics than previous channelrhodopsins yet is effectively more light sensitive. Together these two reagents enable two-color activation of neural spiking and downstream synaptic transmission in independent neural populations without detectable cross-talk in mouse brain slice. |
| first_indexed | 2024-09-23T08:38:02Z |
| format | Article |
| id | mit-1721.1/92352 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:38:02Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/923522021-09-10T15:13:16Z Independent optical excitation of distinct neural populations Klapoetke, Nathan Cao Murata, Yasunobu Kim, Sung Soo Pulver, Stefan R. Birdsey-Benson, Amanda Cho, Yong Ku Morimoto, Tania K. Chuong, Amy S. Carpenter, Eric J. Tian, Zhijian Wang, Jun Xie, Yinlong Yan, Zhixiang Zhang, Yong Chow, Brian Y. Surek, Barbara Melkonian, Michael Jayaraman, Vivek Wong, Gane Ka-Shu Constantine-Paton, Martha Boyden, Edward Massachusetts Institute of Technology. Synthetic Neurobiology Group Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Media Laboratory McGovern Institute for Brain Research at MIT Massachusetts Institute of Technology. Center for Neurobiological Engineering Klapoetke, Nathan Cao Murata, Yasunobu Birdsey-Benson, Amanda Morimoto, Tania K. Chuong, Amy S. Cho, Yong Ku Constantine-Paton, Martha Boyden, Edward Stuart Optogenetic tools enable examination of how specific cell types contribute to brain circuit functions. A long-standing question is whether it is possible to independently activate two distinct neural populations in mammalian brain tissue. Such a capability would enable the study of how different synapses or pathways interact to encode information in the brain. Here we describe two channelrhodopsins, Chronos and Chrimson, discovered through sequencing and physiological characterization of opsins from over 100 species of alga. Chrimson's excitation spectrum is red shifted by 45 nm relative to previous channelrhodopsins and can enable experiments in which red light is preferred. We show minimal visual system–mediated behavioral interference when using the variant CsChrimson in neurobehavioral studies in Drosophila melanogaster. Chronos has faster kinetics than previous channelrhodopsins yet is effectively more light sensitive. Together these two reagents enable two-color activation of neural spiking and downstream synaptic transmission in independent neural populations without detectable cross-talk in mouse brain slice. Howard Hughes Medical Institute Alberta. Alberta Innovation and Advanced Education Alberta Innovates--Technology Futures United States. Defense Advanced Research Projects Agency (DARPA Living Foundries, Contract HR0011-12-C-0068) National Science Foundation (U.S.) (Biophotonics Program) National Institutes of Health (U.S.) (NIH grant 5R01EY014074-18) United States. Dept. of Defense (Office of the Assistant Secretary of Defense for Research and Engineering) Massachusetts Institute of Technology. Media Laboratory National Science Foundation (U.S.) (NSF Harvard/MIT Joint grants in Basic Neuroscience, CBET 1053233) National Science Foundation (U.S.) (NSF Harvard/MIT Joint grants in Basic Neuroscience, EFRI 0835878) National Institutes of Health (U.S.) (grant NIH 1DP2OD002002) National Institutes of Health (U.S.) (grant NIH 1R01NS067199) National Institutes of Health (U.S.) (grant NIH 1R01DA029639) National Institutes of Health (U.S.) (grant NIH 1R01GM104948) National Institutes of Health (U.S.) (grant NIH 1RC1MH088182) National Institutes of Health (U.S.) (grant NIH 1R01NS075421) Wallace H. Coulter Foundation Alfred P. Sloan Foundation Human Frontier Science Program New York Stem Cell Foundation Institution of Engineering and Technology Skolkovo Institute of Science and Technology 2014-12-16T21:46:24Z 2014-12-16T21:46:24Z 2014-02 Article http://purl.org/eprint/type/JournalArticle 1548-7091 1548-7105 http://hdl.handle.net/1721.1/92352 Klapoetke, Nathan C, Yasunobu Murata, Sung Soo Kim, Stefan R Pulver, Amanda Birdsey-Benson, Yong Ku Cho, Tania K Morimoto, et al. “Independent Optical Excitation of Distinct Neural Populations.” Nature Methods 11, no. 3 (February 9, 2014): 338–346. https://orcid.org/0000-0003-2268-0863 https://orcid.org/0000-0002-0419-3351 en_US http://dx.doi.org/10.1038/nmeth.2836 Nature Methods Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Klapoetke, Nathan Cao Murata, Yasunobu Kim, Sung Soo Pulver, Stefan R. Birdsey-Benson, Amanda Cho, Yong Ku Morimoto, Tania K. Chuong, Amy S. Carpenter, Eric J. Tian, Zhijian Wang, Jun Xie, Yinlong Yan, Zhixiang Zhang, Yong Chow, Brian Y. Surek, Barbara Melkonian, Michael Jayaraman, Vivek Wong, Gane Ka-Shu Constantine-Paton, Martha Boyden, Edward Independent optical excitation of distinct neural populations |
| title | Independent optical excitation of distinct neural populations |
| title_full | Independent optical excitation of distinct neural populations |
| title_fullStr | Independent optical excitation of distinct neural populations |
| title_full_unstemmed | Independent optical excitation of distinct neural populations |
| title_short | Independent optical excitation of distinct neural populations |
| title_sort | independent optical excitation of distinct neural populations |
| url | http://hdl.handle.net/1721.1/92352 https://orcid.org/0000-0003-2268-0863 https://orcid.org/0000-0002-0419-3351 |
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