A Septin-Dependent Diffusion Barrier at Dendritic Spine Necks
Excitatory glutamatergic synapses at dendritic spines exchange and modulate their receptor content via lateral membrane diffusion. Several studies have shown that the thin spine neck impedes the access of membrane and solute molecules to the spine head. However, it is unclear whether the spine neck...
| Main Authors: | , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Public Library of Science
2014
|
| Online Access: | http://hdl.handle.net/1721.1/92480 |
| _version_ | 1826211290557710336 |
|---|---|
| author | Ewers, Helge Tada, Tomoko Petersen, Jennifer D. Racz, Bence Sheng, Morgan Choquet, Daniel |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Ewers, Helge Tada, Tomoko Petersen, Jennifer D. Racz, Bence Sheng, Morgan Choquet, Daniel |
| author_sort | Ewers, Helge |
| collection | MIT |
| description | Excitatory glutamatergic synapses at dendritic spines exchange and modulate their receptor content via lateral membrane diffusion. Several studies have shown that the thin spine neck impedes the access of membrane and solute molecules to the spine head. However, it is unclear whether the spine neck geometry alone restricts access to dendritic spines or if a physical barrier to the diffusion of molecules exists. Here, we investigated whether a complex of septin cytoskeletal GTPases localized at the base of the spine neck regulates diffusion across the spine neck. We found that, during development, a marker of the septin complex, Septin7 (Sept7), becomes localized to the spine neck where it forms a stable structure underneath the plasma membrane. We show that diffusion of receptors and bulk membrane, but not cytoplasmic proteins, is slower in spines bearing Sept7 at their neck. Finally, when Sept7 expression was suppressed by RNA interference, membrane molecules explored larger membrane areas. Our findings indicate that Sept7 regulates membrane protein access to spines. |
| first_indexed | 2024-09-23T15:03:35Z |
| format | Article |
| id | mit-1721.1/92480 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:03:35Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/924802022-09-29T12:20:57Z A Septin-Dependent Diffusion Barrier at Dendritic Spine Necks Ewers, Helge Tada, Tomoko Petersen, Jennifer D. Racz, Bence Sheng, Morgan Choquet, Daniel Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Picower Institute for Learning and Memory Tada, Tomoko Excitatory glutamatergic synapses at dendritic spines exchange and modulate their receptor content via lateral membrane diffusion. Several studies have shown that the thin spine neck impedes the access of membrane and solute molecules to the spine head. However, it is unclear whether the spine neck geometry alone restricts access to dendritic spines or if a physical barrier to the diffusion of molecules exists. Here, we investigated whether a complex of septin cytoskeletal GTPases localized at the base of the spine neck regulates diffusion across the spine neck. We found that, during development, a marker of the septin complex, Septin7 (Sept7), becomes localized to the spine neck where it forms a stable structure underneath the plasma membrane. We show that diffusion of receptors and bulk membrane, but not cytoplasmic proteins, is slower in spines bearing Sept7 at their neck. Finally, when Sept7 expression was suppressed by RNA interference, membrane molecules explored larger membrane areas. Our findings indicate that Sept7 regulates membrane protein access to spines. Human Frontier Science Program (Strasbourg, France) (Short-Term Fellowship) 2014-12-23T19:52:29Z 2014-12-23T19:52:29Z 2014-12 2014-04 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/92480 Ewers, Helge, Tomoko Tada, Jennifer D. Petersen, Bence Racz, Morgan Sheng, and Daniel Choquet. “A Septin-Dependent Diffusion Barrier at Dendritic Spine Necks.” Edited by Lin Mei. PLoS ONE 9, no. 12 (December 10, 2014): e113916. en_US http://dx.doi.org/10.1371/journal.pone.0113916 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Ewers, Helge Tada, Tomoko Petersen, Jennifer D. Racz, Bence Sheng, Morgan Choquet, Daniel A Septin-Dependent Diffusion Barrier at Dendritic Spine Necks |
| title | A Septin-Dependent Diffusion Barrier at Dendritic Spine Necks |
| title_full | A Septin-Dependent Diffusion Barrier at Dendritic Spine Necks |
| title_fullStr | A Septin-Dependent Diffusion Barrier at Dendritic Spine Necks |
| title_full_unstemmed | A Septin-Dependent Diffusion Barrier at Dendritic Spine Necks |
| title_short | A Septin-Dependent Diffusion Barrier at Dendritic Spine Necks |
| title_sort | septin dependent diffusion barrier at dendritic spine necks |
| url | http://hdl.handle.net/1721.1/92480 |
| work_keys_str_mv | AT ewershelge aseptindependentdiffusionbarrieratdendriticspinenecks AT tadatomoko aseptindependentdiffusionbarrieratdendriticspinenecks AT petersenjenniferd aseptindependentdiffusionbarrieratdendriticspinenecks AT raczbence aseptindependentdiffusionbarrieratdendriticspinenecks AT shengmorgan aseptindependentdiffusionbarrieratdendriticspinenecks AT choquetdaniel aseptindependentdiffusionbarrieratdendriticspinenecks AT ewershelge septindependentdiffusionbarrieratdendriticspinenecks AT tadatomoko septindependentdiffusionbarrieratdendriticspinenecks AT petersenjenniferd septindependentdiffusionbarrieratdendriticspinenecks AT raczbence septindependentdiffusionbarrieratdendriticspinenecks AT shengmorgan septindependentdiffusionbarrieratdendriticspinenecks AT choquetdaniel septindependentdiffusionbarrieratdendriticspinenecks |