Real-time in vivo optogenetic neuromodulation and multielectrode electrophysiologic recording with NeuroRighter
Optogenetic channels have greatly expanded neuroscience’s experimental capabilities, enabling precise genetic targeting and manipulation of neuron subpopulations in awake and behaving animals. However, many barriers to entry remain for this technology – including low-cost and effective hardware for...
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| Format: | Article |
| Language: | en_US |
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Frontiers Research Foundation
2014
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| Online Access: | http://hdl.handle.net/1721.1/92487 https://orcid.org/0000-0002-5425-3340 |
| _version_ | 1826201279912738816 |
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| author | Laxpati, Nealen G. Mahmoudi, Babak Gutekunst, Claire-Anne Newman, Jonathan P. Zeller-Townson, Riley Gross, Robert E. |
| author2 | Picower Institute for Learning and Memory |
| author_facet | Picower Institute for Learning and Memory Laxpati, Nealen G. Mahmoudi, Babak Gutekunst, Claire-Anne Newman, Jonathan P. Zeller-Townson, Riley Gross, Robert E. |
| author_sort | Laxpati, Nealen G. |
| collection | MIT |
| description | Optogenetic channels have greatly expanded neuroscience’s experimental capabilities, enabling precise genetic targeting and manipulation of neuron subpopulations in awake and behaving animals. However, many barriers to entry remain for this technology – including low-cost and effective hardware for combined optical stimulation and electrophysiologic recording. To address this, we adapted the open-source NeuroRighter multichannel electrophysiology platform for use in awake and behaving rodents in both open and closed-loop stimulation experiments. Here, we present these cost-effective adaptations, including commercially available LED light sources; custom-made optical ferrules; 3D printed ferrule hardware and software to calibrate and standardize output intensity; and modifications to commercially available electrode arrays enabling stimulation proximally and distally to the recording target. We then demonstrate the capabilities and versatility of these adaptations in several open and closed-loop experiments, demonstrate spectrographic methods of analyzing the results, as well as discuss artifacts of stimulation. |
| first_indexed | 2024-09-23T11:49:32Z |
| format | Article |
| id | mit-1721.1/92487 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:49:32Z |
| publishDate | 2014 |
| publisher | Frontiers Research Foundation |
| record_format | dspace |
| spelling | mit-1721.1/924872022-09-27T22:08:50Z Real-time in vivo optogenetic neuromodulation and multielectrode electrophysiologic recording with NeuroRighter Laxpati, Nealen G. Mahmoudi, Babak Gutekunst, Claire-Anne Newman, Jonathan P. Zeller-Townson, Riley Gross, Robert E. Picower Institute for Learning and Memory Newman, Jonathan P. Optogenetic channels have greatly expanded neuroscience’s experimental capabilities, enabling precise genetic targeting and manipulation of neuron subpopulations in awake and behaving animals. However, many barriers to entry remain for this technology – including low-cost and effective hardware for combined optical stimulation and electrophysiologic recording. To address this, we adapted the open-source NeuroRighter multichannel electrophysiology platform for use in awake and behaving rodents in both open and closed-loop stimulation experiments. Here, we present these cost-effective adaptations, including commercially available LED light sources; custom-made optical ferrules; 3D printed ferrule hardware and software to calibrate and standardize output intensity; and modifications to commercially available electrode arrays enabling stimulation proximally and distally to the recording target. We then demonstrate the capabilities and versatility of these adaptations in several open and closed-loop experiments, demonstrate spectrographic methods of analyzing the results, as well as discuss artifacts of stimulation. Emory University. School of Medicine (Emory Neurosciences Initiative seed grant) American Epilepsy Society Epilepsy Foundation of America (Predoctoral fellowship) National Science Foundation (U.S.) (NSF GRFP Fellowship 08-593) National Science Foundation (U.S.) (NSF IGERT Fellowship DGE-0333411) National Science Foundation (U.S.) (NSF EFRI #1238097) National Institutes of Health (U.S.) (NIH 1R01NS079757-01) American Society for Engineering Education (SMART Fellowship) 2014-12-23T20:47:19Z 2014-12-23T20:47:19Z 2014-10 Article http://purl.org/eprint/type/JournalArticle 1662-6443 1662-453X http://hdl.handle.net/1721.1/92487 Laxpati, Nealen G., Babak Mahmoudi, Claire-Anne Gutekunst, Jonathan P. Newman, Riley Zeller-Townson, and Robert E. Gross. “Real-Time in Vivo Optogenetic Neuromodulation and Multielectrode Electrophysiologic Recording with NeuroRighter.” Frontiers in Neuroengineering 7 (October 29, 2014). https://orcid.org/0000-0002-5425-3340 en_US http://dx.doi.org/10.3389/fneng.2014.00040 Frontiers in Neuroengineering Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Frontiers Research Foundation Frontiers Research Foundation |
| spellingShingle | Laxpati, Nealen G. Mahmoudi, Babak Gutekunst, Claire-Anne Newman, Jonathan P. Zeller-Townson, Riley Gross, Robert E. Real-time in vivo optogenetic neuromodulation and multielectrode electrophysiologic recording with NeuroRighter |
| title | Real-time in vivo optogenetic neuromodulation and multielectrode electrophysiologic recording with NeuroRighter |
| title_full | Real-time in vivo optogenetic neuromodulation and multielectrode electrophysiologic recording with NeuroRighter |
| title_fullStr | Real-time in vivo optogenetic neuromodulation and multielectrode electrophysiologic recording with NeuroRighter |
| title_full_unstemmed | Real-time in vivo optogenetic neuromodulation and multielectrode electrophysiologic recording with NeuroRighter |
| title_short | Real-time in vivo optogenetic neuromodulation and multielectrode electrophysiologic recording with NeuroRighter |
| title_sort | real time in vivo optogenetic neuromodulation and multielectrode electrophysiologic recording with neurorighter |
| url | http://hdl.handle.net/1721.1/92487 https://orcid.org/0000-0002-5425-3340 |
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