Improving femtosecond laser pulse delivery through a hollow core photonic crystal fiber for temporally focused two-photon endomicroscopy
In this paper, we present a strategy to improve delivery of femtosecond laser pulses from a regenerative amplifier through a hollow core photonic crystal fiber for temporally focused wide-field two-photon endomicroscopy. For endomicroscope application, wide-field two-photon excitation has the advant...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/92549 https://orcid.org/0000-0003-3681-7410 https://orcid.org/0000-0003-4698-6488 |
| _version_ | 1826203169224392704 |
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| author | Choi, Heejin So, Peter T. C. |
| author2 | Institute for Medical Engineering and Science |
| author_facet | Institute for Medical Engineering and Science Choi, Heejin So, Peter T. C. |
| author_sort | Choi, Heejin |
| collection | MIT |
| description | In this paper, we present a strategy to improve delivery of femtosecond laser pulses from a regenerative amplifier through a hollow core photonic crystal fiber for temporally focused wide-field two-photon endomicroscopy. For endomicroscope application, wide-field two-photon excitation has the advantage of requiring no scanning in the distal end. However, wide-field two-photon excitation requires peak power that is 10[superscript 4]–10[superscript 5] times higher than the point scanning approach corresponding to femtosecond pulses with energy on the order of 1–10 μJ at the specimen plane. The transmission of these high energy pulses through a single mode fiber into the microendoscope is a significant challenge. Two approaches were pursued to partially overcome this limitation. First, a single high energy pulse is split into a train of pulses with energy below the fiber damage threshold better utilizing the available laser energy. Second, stretching the pulse width in time by introducing negative dispersion was shown to have the dual benefit of reducing fiber damage probability and compensating for the positive group velocity dispersion induced by the fiber. With these strategy applied, 11 fold increase in the two photon excitation signal has been demonstrated. |
| first_indexed | 2024-09-23T12:32:25Z |
| format | Article |
| id | mit-1721.1/92549 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:32:25Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/925492022-10-01T09:38:55Z Improving femtosecond laser pulse delivery through a hollow core photonic crystal fiber for temporally focused two-photon endomicroscopy Choi, Heejin So, Peter T. C. Institute for Medical Engineering and Science Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Laser Biomedical Research Center So, Peter T. C. Choi, Heejin In this paper, we present a strategy to improve delivery of femtosecond laser pulses from a regenerative amplifier through a hollow core photonic crystal fiber for temporally focused wide-field two-photon endomicroscopy. For endomicroscope application, wide-field two-photon excitation has the advantage of requiring no scanning in the distal end. However, wide-field two-photon excitation requires peak power that is 10[superscript 4]–10[superscript 5] times higher than the point scanning approach corresponding to femtosecond pulses with energy on the order of 1–10 μJ at the specimen plane. The transmission of these high energy pulses through a single mode fiber into the microendoscope is a significant challenge. Two approaches were pursued to partially overcome this limitation. First, a single high energy pulse is split into a train of pulses with energy below the fiber damage threshold better utilizing the available laser energy. Second, stretching the pulse width in time by introducing negative dispersion was shown to have the dual benefit of reducing fiber damage probability and compensating for the positive group velocity dispersion induced by the fiber. With these strategy applied, 11 fold increase in the two photon excitation signal has been demonstrated. National Institutes of Health (U.S.) (9P41EB015871-26A1) National Institutes of Health (U.S.) (5R01EY017656-02) National Institutes of Health (U.S.) (5R01 NS051320) National Institutes of Health (U.S.) (4R44EB012415-02) National Science Foundation (U.S.) (CBET-0939511) Singapore-MIT Alliance Singapore-MIT Alliance for Research and Technology Skolkovo Institute of Science and Technology Hamamatsu Corporation David H. Koch Institute for Integrative Cancer Research at MIT. Bridge Project Initiative 2014-12-29T22:57:26Z 2014-12-29T22:57:26Z 2014-10 2014-06 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/92549 Choi, Heejin, and Peter T. C. So. “Improving Femtosecond Laser Pulse Delivery through a Hollow Core Photonic Crystal Fiber for Temporally Focused Two-Photon Endomicroscopy.” Sci. Rep. 4 (October 15, 2014): 6626. https://orcid.org/0000-0003-3681-7410 https://orcid.org/0000-0003-4698-6488 en_US http://dx.doi.org/10.1038/srep06626 Scientific Reports Creative Commons Attribution http:// creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Choi, Heejin So, Peter T. C. Improving femtosecond laser pulse delivery through a hollow core photonic crystal fiber for temporally focused two-photon endomicroscopy |
| title | Improving femtosecond laser pulse delivery through a hollow core photonic crystal fiber for temporally focused two-photon endomicroscopy |
| title_full | Improving femtosecond laser pulse delivery through a hollow core photonic crystal fiber for temporally focused two-photon endomicroscopy |
| title_fullStr | Improving femtosecond laser pulse delivery through a hollow core photonic crystal fiber for temporally focused two-photon endomicroscopy |
| title_full_unstemmed | Improving femtosecond laser pulse delivery through a hollow core photonic crystal fiber for temporally focused two-photon endomicroscopy |
| title_short | Improving femtosecond laser pulse delivery through a hollow core photonic crystal fiber for temporally focused two-photon endomicroscopy |
| title_sort | improving femtosecond laser pulse delivery through a hollow core photonic crystal fiber for temporally focused two photon endomicroscopy |
| url | http://hdl.handle.net/1721.1/92549 https://orcid.org/0000-0003-3681-7410 https://orcid.org/0000-0003-4698-6488 |
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