Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells
Although cortical actin plays an important role in cellular mechanics and morphogenesis, there is surprisingly little information on cortex organization at the apical surface of cells. In this paper, we characterize organization and dynamics of microvilli (MV) and a previously unappreciated actomyos...
| প্রধান লেখক: | , , , , , , , , , , |
|---|---|
| অন্যান্য লেখক: | |
| বিন্যাস: | প্রবন্ধ |
| ভাষা: | en_US |
| প্রকাশিত: |
Rockefeller University Press, The
2014
|
| অনলাইন ব্যবহার করুন: | http://hdl.handle.net/1721.1/92555 https://orcid.org/0000-0002-6199-6855 |
| _version_ | 1826201675704041472 |
|---|---|
| author | Bathe, Mark Diesinger, Philipp M. Klingner, Christoph Cherian, Anoop V. Fels, Johannes Aufschnaiter, Roland Maghelli, Nicola Keil, Thomas Beck, Gisela Tolic-Norrelykke, Iva M. Wedlich-Soldner, Roland |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Bathe, Mark Diesinger, Philipp M. Klingner, Christoph Cherian, Anoop V. Fels, Johannes Aufschnaiter, Roland Maghelli, Nicola Keil, Thomas Beck, Gisela Tolic-Norrelykke, Iva M. Wedlich-Soldner, Roland |
| author_sort | Bathe, Mark |
| collection | MIT |
| description | Although cortical actin plays an important role in cellular mechanics and morphogenesis, there is surprisingly little information on cortex organization at the apical surface of cells. In this paper, we characterize organization and dynamics of microvilli (MV) and a previously unappreciated actomyosin network at the apical surface of Madin–Darby canine kidney cells. In contrast to short and static MV in confluent cells, the apical surfaces of nonconfluent epithelial cells (ECs) form highly dynamic protrusions, which are often oriented along the plane of the membrane. These dynamic MV exhibit complex and spatially correlated reorganization, which is dependent on myosin II activity. Surprisingly, myosin II is organized into an extensive network of filaments spanning the entire apical membrane in nonconfluent ECs. Dynamic MV, myosin filaments, and their associated actin filaments form an interconnected, prestressed network. Interestingly, this network regulates lateral mobility of apical membrane probes such as integrins or epidermal growth factor receptors, suggesting that coordinated actomyosin dynamics contributes to apical cell membrane organization. |
| first_indexed | 2024-09-23T11:55:20Z |
| format | Article |
| id | mit-1721.1/92555 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:55:20Z |
| publishDate | 2014 |
| publisher | Rockefeller University Press, The |
| record_format | dspace |
| spelling | mit-1721.1/925552022-10-01T06:57:26Z Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells Bathe, Mark Diesinger, Philipp M. Klingner, Christoph Cherian, Anoop V. Fels, Johannes Aufschnaiter, Roland Maghelli, Nicola Keil, Thomas Beck, Gisela Tolic-Norrelykke, Iva M. Wedlich-Soldner, Roland Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Laboratory for Computational Cell Biology & Biophysics Bathe, Mark Diesinger, Philipp M. Although cortical actin plays an important role in cellular mechanics and morphogenesis, there is surprisingly little information on cortex organization at the apical surface of cells. In this paper, we characterize organization and dynamics of microvilli (MV) and a previously unappreciated actomyosin network at the apical surface of Madin–Darby canine kidney cells. In contrast to short and static MV in confluent cells, the apical surfaces of nonconfluent epithelial cells (ECs) form highly dynamic protrusions, which are often oriented along the plane of the membrane. These dynamic MV exhibit complex and spatially correlated reorganization, which is dependent on myosin II activity. Surprisingly, myosin II is organized into an extensive network of filaments spanning the entire apical membrane in nonconfluent ECs. Dynamic MV, myosin filaments, and their associated actin filaments form an interconnected, prestressed network. Interestingly, this network regulates lateral mobility of apical membrane probes such as integrins or epidermal growth factor receptors, suggesting that coordinated actomyosin dynamics contributes to apical cell membrane organization. Max Planck Society for the Advancement of Science German Research Foundation (SFB863) University of Münster. Cells-in-Motion Cluster of Excellence (EXC1003-CiM) 2014-12-30T19:05:14Z 2014-12-30T19:05:14Z 2014-10 2014-02 Article http://purl.org/eprint/type/JournalArticle 0021-9525 1540-8140 http://hdl.handle.net/1721.1/92555 Klingner, Christoph, Anoop V. Cherian, Johannes Fels, Philipp M. Diesinger, Roland Aufschnaiter, Nicola Maghelli, Thomas Keil, et al. “Isotropic Actomyosin Dynamics Promote Organization of the Apical Cell Cortex in Epithelial Cells.” The Journal of Cell Biology 207, no. 1 (October 13, 2014): 107–121. https://orcid.org/0000-0002-6199-6855 en_US http://dx.doi.org/10.1083/jcb.201402037 Journal of Cell Biology Creative Commons Attribution http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Rockefeller University Press, The Oxford University Press |
| spellingShingle | Bathe, Mark Diesinger, Philipp M. Klingner, Christoph Cherian, Anoop V. Fels, Johannes Aufschnaiter, Roland Maghelli, Nicola Keil, Thomas Beck, Gisela Tolic-Norrelykke, Iva M. Wedlich-Soldner, Roland Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells |
| title | Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells |
| title_full | Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells |
| title_fullStr | Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells |
| title_full_unstemmed | Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells |
| title_short | Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells |
| title_sort | isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells |
| url | http://hdl.handle.net/1721.1/92555 https://orcid.org/0000-0002-6199-6855 |
| work_keys_str_mv | AT bathemark isotropicactomyosindynamicspromoteorganizationoftheapicalcellcortexinepithelialcells AT diesingerphilippm isotropicactomyosindynamicspromoteorganizationoftheapicalcellcortexinepithelialcells AT klingnerchristoph isotropicactomyosindynamicspromoteorganizationoftheapicalcellcortexinepithelialcells AT cheriananoopv isotropicactomyosindynamicspromoteorganizationoftheapicalcellcortexinepithelialcells AT felsjohannes isotropicactomyosindynamicspromoteorganizationoftheapicalcellcortexinepithelialcells AT aufschnaiterroland isotropicactomyosindynamicspromoteorganizationoftheapicalcellcortexinepithelialcells AT maghellinicola isotropicactomyosindynamicspromoteorganizationoftheapicalcellcortexinepithelialcells AT keilthomas isotropicactomyosindynamicspromoteorganizationoftheapicalcellcortexinepithelialcells AT beckgisela isotropicactomyosindynamicspromoteorganizationoftheapicalcellcortexinepithelialcells AT tolicnorrelykkeivam isotropicactomyosindynamicspromoteorganizationoftheapicalcellcortexinepithelialcells AT wedlichsoldnerroland isotropicactomyosindynamicspromoteorganizationoftheapicalcellcortexinepithelialcells |