Live visualizations of single isolated tubulin protein self-assembly via tunneling current: effect of electromagnetic pumping during spontaneous growth of microtubule
As we bring tubulin protein molecules one by one into the vicinity, they self-assemble and entire event we capture live via quantum tunneling. We observe how these molecules form a linear chain and then chains self-assemble into 2D sheet, an essential for microtubule, —fundamental nano-tube in a cel...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/92556 |
| _version_ | 1826199928168251392 |
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| author | Sahu, Satyajit Ghosh, Subrata K. Fujita, Daisuke Bandyopadhyay, Anirban |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Sahu, Satyajit Ghosh, Subrata K. Fujita, Daisuke Bandyopadhyay, Anirban |
| author_sort | Sahu, Satyajit |
| collection | MIT |
| description | As we bring tubulin protein molecules one by one into the vicinity, they self-assemble and entire event we capture live via quantum tunneling. We observe how these molecules form a linear chain and then chains self-assemble into 2D sheet, an essential for microtubule, —fundamental nano-tube in a cellular life form. Even without using GTP, or any chemical reaction, but applying particular ac signal using specially designed antenna around atomic sharp tip we could carry out the self-assembly, however, if there is no electromagnetic pumping, no self-assembly is observed. In order to verify this atomic scale observation, we have built an artificial cell-like environment with nano-scale engineering and repeated spontaneous growth of tubulin protein to its complex with and without electromagnetic signal. We used 64 combinations of plant, animal and fungi tubulins and several doping molecules used as drug, and repeatedly observed that the long reported common frequency region where protein folds mechanically and its structures vibrate electromagnetically. Under pumping, the growth process exhibits a unique organized behavior unprecedented otherwise. Thus, “common frequency point” is proposed as a tool to regulate protein complex related diseases in the future. |
| first_indexed | 2024-09-23T11:28:02Z |
| format | Article |
| id | mit-1721.1/92556 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:28:02Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/925562024-03-20T19:45:23Z Live visualizations of single isolated tubulin protein self-assembly via tunneling current: effect of electromagnetic pumping during spontaneous growth of microtubule Sahu, Satyajit Ghosh, Subrata K. Fujita, Daisuke Bandyopadhyay, Anirban Massachusetts Institute of Technology. Institute for Medical Engineering & Science Harvard University--MIT Division of Health Sciences and Technology Bandyopadhyay, Anirban As we bring tubulin protein molecules one by one into the vicinity, they self-assemble and entire event we capture live via quantum tunneling. We observe how these molecules form a linear chain and then chains self-assemble into 2D sheet, an essential for microtubule, —fundamental nano-tube in a cellular life form. Even without using GTP, or any chemical reaction, but applying particular ac signal using specially designed antenna around atomic sharp tip we could carry out the self-assembly, however, if there is no electromagnetic pumping, no self-assembly is observed. In order to verify this atomic scale observation, we have built an artificial cell-like environment with nano-scale engineering and repeated spontaneous growth of tubulin protein to its complex with and without electromagnetic signal. We used 64 combinations of plant, animal and fungi tubulins and several doping molecules used as drug, and repeatedly observed that the long reported common frequency region where protein folds mechanically and its structures vibrate electromagnetically. Under pumping, the growth process exhibits a unique organized behavior unprecedented otherwise. Thus, “common frequency point” is proposed as a tool to regulate protein complex related diseases in the future. Japan. Ministry of Education, Culture, Sports, Science and Technology. Asian Office of Aerospace Research and Development (AOARD) (FA2386-11-1-0001AOARD104173) Asian Office of Aerospace Research and Development (AOARD) (FA2386-10-1-4059 AOARD-10-4059) 2014-12-30T19:16:37Z 2014-12-30T19:16:37Z 2014-12 2014-07 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/92556 Sahu, Satyajit, Subrata Ghosh, Daisuke Fujita, and Anirban Bandyopadhyay. “Live Visualizations of Single Isolated Tubulin Protein Self-Assembly via Tunneling Current: Effect of Electromagnetic Pumping During Spontaneous Growth of Microtubule.” Sci. Rep. 4 (December 3, 2014): 7303. en_US http://dx.doi.org/10.1038/srep07303 Scientific Reports Creative Commons Attribution http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Sahu, Satyajit Ghosh, Subrata K. Fujita, Daisuke Bandyopadhyay, Anirban Live visualizations of single isolated tubulin protein self-assembly via tunneling current: effect of electromagnetic pumping during spontaneous growth of microtubule |
| title | Live visualizations of single isolated tubulin protein self-assembly via tunneling current: effect of electromagnetic pumping during spontaneous growth of microtubule |
| title_full | Live visualizations of single isolated tubulin protein self-assembly via tunneling current: effect of electromagnetic pumping during spontaneous growth of microtubule |
| title_fullStr | Live visualizations of single isolated tubulin protein self-assembly via tunneling current: effect of electromagnetic pumping during spontaneous growth of microtubule |
| title_full_unstemmed | Live visualizations of single isolated tubulin protein self-assembly via tunneling current: effect of electromagnetic pumping during spontaneous growth of microtubule |
| title_short | Live visualizations of single isolated tubulin protein self-assembly via tunneling current: effect of electromagnetic pumping during spontaneous growth of microtubule |
| title_sort | live visualizations of single isolated tubulin protein self assembly via tunneling current effect of electromagnetic pumping during spontaneous growth of microtubule |
| url | http://hdl.handle.net/1721.1/92556 |
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