Zebrafish as a model to study live mucus physiology
Dysfunctional mucus barriers can result in important pulmonary and gastrointestinal conditions, but model systems to study the underlying causes are largely missing. We identified and characterized five mucin homologues in zebrafish, and demonstrated a strategy for fluorescence labeling of one selec...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/92571 https://orcid.org/0000-0001-8260-338X |
| _version_ | 1826203349603581952 |
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| author | Jevtov, Irena Samuelsson, Tore Yao, Grace Amsterdam, Adam Ribbeck, Katharina |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Jevtov, Irena Samuelsson, Tore Yao, Grace Amsterdam, Adam Ribbeck, Katharina |
| author_sort | Jevtov, Irena |
| collection | MIT |
| description | Dysfunctional mucus barriers can result in important pulmonary and gastrointestinal conditions, but model systems to study the underlying causes are largely missing. We identified and characterized five mucin homologues in zebrafish, and demonstrated a strategy for fluorescence labeling of one selected mucin. These tools can be used for in vivo experiments and in pharmacological and genetic screens to study the dynamics and mechanisms of mucosal physiology. |
| first_indexed | 2024-09-23T12:35:13Z |
| format | Article |
| id | mit-1721.1/92571 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:35:13Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/925712022-10-01T09:55:01Z Zebrafish as a model to study live mucus physiology Jevtov, Irena Samuelsson, Tore Yao, Grace Amsterdam, Adam Ribbeck, Katharina Massachusetts Institute of Technology. Department of Biological Engineering Koch Institute for Integrative Cancer Research at MIT Amsterdam, Adam Ribbeck, Katharina Jevtov, Irena Yao, Grace Dysfunctional mucus barriers can result in important pulmonary and gastrointestinal conditions, but model systems to study the underlying causes are largely missing. We identified and characterized five mucin homologues in zebrafish, and demonstrated a strategy for fluorescence labeling of one selected mucin. These tools can be used for in vivo experiments and in pharmacological and genetic screens to study the dynamics and mechanisms of mucosal physiology. National Institute of Environmental Health Sciences (Grant P30-ES002109) Johnson & Johnson. Corporate Office of Science and Technology National Institutes of Health (U.S.) (Grant CA106416) Kathy and Curt Marble Cancer Research Fund David H. Koch Institute for Integrative Cancer Research at MIT (Zebrafish Core Facility) 2014-12-31T20:06:59Z 2014-12-31T20:06:59Z 2014-10 2014-04 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/92571 Jevtov, Irena, Tore Samuelsson, Grace Yao, Adam Amsterdam, and Katharina Ribbeck. “Zebrafish as a Model to Study Live Mucus Physiology.” Sci. Rep. 4 (October 17, 2014): 6653. https://orcid.org/0000-0001-8260-338X en_US http://dx.doi.org/10.1038/srep06653 Scientific Reports Creative Commons Attribution http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Jevtov, Irena Samuelsson, Tore Yao, Grace Amsterdam, Adam Ribbeck, Katharina Zebrafish as a model to study live mucus physiology |
| title | Zebrafish as a model to study live mucus physiology |
| title_full | Zebrafish as a model to study live mucus physiology |
| title_fullStr | Zebrafish as a model to study live mucus physiology |
| title_full_unstemmed | Zebrafish as a model to study live mucus physiology |
| title_short | Zebrafish as a model to study live mucus physiology |
| title_sort | zebrafish as a model to study live mucus physiology |
| url | http://hdl.handle.net/1721.1/92571 https://orcid.org/0000-0001-8260-338X |
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