Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2
A major long-term goal of systems neuroscience is to identify the different roles of neural subtypes in brain circuit function. The ability to causally manipulate selective cell types is critical to meeting this goal. This protocol describes techniques for optically stimulating specific populations...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2015
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| Online Access: | http://hdl.handle.net/1721.1/92883 https://orcid.org/0000-0003-1262-0592 https://orcid.org/0000-0002-0756-5587 |
| _version_ | 1811091690399727616 |
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| author | Knoblich, Ulf Zhang, Feng Deisseroth, Karl Tsai, Li-Huei Cardin, Jessica A. Carlen, Marie Meletis, Konstantinos Moore, Christopher I. |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Knoblich, Ulf Zhang, Feng Deisseroth, Karl Tsai, Li-Huei Cardin, Jessica A. Carlen, Marie Meletis, Konstantinos Moore, Christopher I. |
| author_sort | Knoblich, Ulf |
| collection | MIT |
| description | A major long-term goal of systems neuroscience is to identify the different roles of neural subtypes in brain circuit function. The ability to causally manipulate selective cell types is critical to meeting this goal. This protocol describes techniques for optically stimulating specific populations of excitatory neurons and inhibitory interneurons in vivo in combination with electrophysiology. Cell type selectivity is obtained using Cre-dependent expression of the light-activated channel Channelrhodopsin-2. We also describe approaches for minimizing optical interference with simultaneous extracellular and intracellular recording. These optogenetic techniques provide a spatially and temporally precise means of studying neural activity in the intact brain and allow a detailed examination of the effect of evoked activity on the surrounding local neural network. Injection of viral vectors requires 30–45 min, and in vivo electrophysiology with optogenetic stimulation requires 1–4 h. |
| first_indexed | 2024-09-23T15:06:24Z |
| format | Article |
| id | mit-1721.1/92883 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:06:24Z |
| publishDate | 2015 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/928832022-09-29T12:43:53Z Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2 Knoblich, Ulf Zhang, Feng Deisseroth, Karl Tsai, Li-Huei Cardin, Jessica A. Carlen, Marie Meletis, Konstantinos Moore, Christopher I. Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Picower Institute for Learning and Memory Tsai, Li-Huei Cardin, Jessica A. Carlen, Marie Meletis, Konstantinos Moore, Christopher I. Knoblich, Ulf A major long-term goal of systems neuroscience is to identify the different roles of neural subtypes in brain circuit function. The ability to causally manipulate selective cell types is critical to meeting this goal. This protocol describes techniques for optically stimulating specific populations of excitatory neurons and inhibitory interneurons in vivo in combination with electrophysiology. Cell type selectivity is obtained using Cre-dependent expression of the light-activated channel Channelrhodopsin-2. We also describe approaches for minimizing optical interference with simultaneous extracellular and intracellular recording. These optogenetic techniques provide a spatially and temporally precise means of studying neural activity in the intact brain and allow a detailed examination of the effect of evoked activity on the surrounding local neural network. Injection of viral vectors requires 30–45 min, and in vivo electrophysiology with optogenetic stimulation requires 1–4 h. National Institutes of Health (U.S.) National Science Foundation (U.S.) Simons Foundation National Institutes of Health (U.S.). Pioneer Award National Eye Institue (K99 Award) Knut and Alice Wallenberg Foundation (Postdoctoral Fellowship) Brain & Behavior Research Foundation. Young Investigator Award Thomas F. Petersen 2015-01-15T16:51:17Z 2015-01-15T16:51:17Z 2010-01 Article http://purl.org/eprint/type/JournalArticle 1754-2189 1750-2799 http://hdl.handle.net/1721.1/92883 Cardin, Jessica A, Marie Carlen, Konstantinos Meletis, Ulf Knoblich, Feng Zhang, Karl Deisseroth, Li-Huei Tsai, and Christopher I Moore. “Targeted Optogenetic Stimulation and Recording of Neurons in Vivo Using Cell-Type-Specific Expression of Channelrhodopsin-2.” Nat Protoc 5, no. 2 (January 21, 2010): 247–254. https://orcid.org/0000-0003-1262-0592 https://orcid.org/0000-0002-0756-5587 en_US http://dx.doi.org/10.1038/nprot.2009.228 Nature Protocols Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Nature Publishing Group PMC |
| spellingShingle | Knoblich, Ulf Zhang, Feng Deisseroth, Karl Tsai, Li-Huei Cardin, Jessica A. Carlen, Marie Meletis, Konstantinos Moore, Christopher I. Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2 |
| title | Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2 |
| title_full | Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2 |
| title_fullStr | Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2 |
| title_full_unstemmed | Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2 |
| title_short | Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2 |
| title_sort | targeted optogenetic stimulation and recording of neurons in vivo using cell type specific expression of channelrhodopsin 2 |
| url | http://hdl.handle.net/1721.1/92883 https://orcid.org/0000-0003-1262-0592 https://orcid.org/0000-0002-0756-5587 |
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