Design, prototyping, and testing of an apparatus for establishing a linear temperature gradient in experimental fish tanks
Thesis: S.B., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2014.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2015
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| Online Access: | http://hdl.handle.net/1721.1/93012 |
| _version_ | 1811079998669324288 |
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| author | Kadri, Romi Sinclair |
| author2 | Anette E. Hosoi. |
| author_facet | Anette E. Hosoi. Kadri, Romi Sinclair |
| author_sort | Kadri, Romi Sinclair |
| collection | MIT |
| description | Thesis: S.B., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2014. |
| first_indexed | 2024-09-23T11:23:53Z |
| format | Thesis |
| id | mit-1721.1/93012 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T11:23:53Z |
| publishDate | 2015 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/930122019-04-10T23:50:20Z Design, prototyping, and testing of an apparatus for establishing a linear temperature gradient in experimental fish tanks Kadri, Romi Sinclair Anette E. Hosoi. Massachusetts Institute of Technology. Department of Mechanical Engineering. Massachusetts Institute of Technology. Department of Mechanical Engineering. Mechanical Engineering. Thesis: S.B., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2014. Cataloged from PDF version of thesis. Includes bibliographical references (page 30). Immunology researchers require a new type of fish tank that provides a linear thermal gradient for experimental zebrafish in order to improve the accuracy and validity of their research. Zebrafish require the ability to select their surrounding water temperature in order to react to a simulated viral infection with an optimized immune response. Although countless immunology studies have been performed with zebrafish to date, their validity came into question in early 2013 when it was demonstrated by MacKenzie et. at that the immune response in fish is critically coupled with a form of environmental temperature selection known as behavioral fever. Current research tanks feature a uniform temperature throughout, preventing the fish from being able to "choose" their surrounding water temperature in response to a simulated viral infection. "Fish that are not offered a choice of temperatures and that therefore cannot express behavioral fever show decreased survival under viral challenge." (MacKenzie, et al., 2013) In this study, a conceptual thermal-fluid system was designed, built, and tested for its ability to establish a stable, linear thermal gradient in a standardsize 10L laboratory tank. The thermal profile of the volume of water contained within the experimental tank was determined from temperature measurements taken at incremental depths. The apparatus was found to produce a suitably linear temperature gradient over a base-to-surface temperature difference of 19.6°0C; far greater than the temperature difference of 8°C necessary for unrestricted behavioral fever to be expressed in experimental zebrafish. As such, it was determined that the approach taken is suitable for future use in the development of aquaria for immunology research and water tanks for other applications. by Romi Sinclair Kadri. S.B. 2015-01-20T17:53:45Z 2015-01-20T17:53:45Z 2014 2014 Thesis http://hdl.handle.net/1721.1/93012 899211570 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 39 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Kadri, Romi Sinclair Design, prototyping, and testing of an apparatus for establishing a linear temperature gradient in experimental fish tanks |
| title | Design, prototyping, and testing of an apparatus for establishing a linear temperature gradient in experimental fish tanks |
| title_full | Design, prototyping, and testing of an apparatus for establishing a linear temperature gradient in experimental fish tanks |
| title_fullStr | Design, prototyping, and testing of an apparatus for establishing a linear temperature gradient in experimental fish tanks |
| title_full_unstemmed | Design, prototyping, and testing of an apparatus for establishing a linear temperature gradient in experimental fish tanks |
| title_short | Design, prototyping, and testing of an apparatus for establishing a linear temperature gradient in experimental fish tanks |
| title_sort | design prototyping and testing of an apparatus for establishing a linear temperature gradient in experimental fish tanks |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/93012 |
| work_keys_str_mv | AT kadriromisinclair designprototypingandtestingofanapparatusforestablishingalineartemperaturegradientinexperimentalfishtanks |