Balancing the Initiation and Molecular Recognition Capabilities of Eosin Macroinitiators of Polymerization-Based Signal Amplification Reactions
Coupling polymerization initiators to molecular recognition events provide the ability to amplify these events and detect them using the formation of a cross-linked polymer as an inexpensive readout that is visible to the unaided eye. The eosin-tertiary amine co-initiation system, activated by visib...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Wiley Blackwell
2015
|
| Online Access: | http://hdl.handle.net/1721.1/94335 https://orcid.org/0000-0002-7096-138X |
| _version_ | 1826213917698818048 |
|---|---|
| author | Lee, Jungkyu K. Sikes, Hadley |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Lee, Jungkyu K. Sikes, Hadley |
| author_sort | Lee, Jungkyu K. |
| collection | MIT |
| description | Coupling polymerization initiators to molecular recognition events provide the ability to amplify these events and detect them using the formation of a cross-linked polymer as an inexpensive readout that is visible to the unaided eye. The eosin-tertiary amine co-initiation system, activated by visible light, has proven utility in this context when an average of three eosin molecules are coupled to a protein detection reagent. The present work addresses the question of how detection sensitivity is impacted when the number of eosin molecules per binding event increases in the range of two to fifteen. Unlike in other initiation systems, a non-monotonic relationship is observed between the number of initiators per binding event and the observed detection sensitivity. |
| first_indexed | 2024-09-23T15:56:54Z |
| format | Article |
| id | mit-1721.1/94335 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:56:54Z |
| publishDate | 2015 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/943352022-09-29T17:12:37Z Balancing the Initiation and Molecular Recognition Capabilities of Eosin Macroinitiators of Polymerization-Based Signal Amplification Reactions Lee, Jungkyu K. Sikes, Hadley Massachusetts Institute of Technology. Department of Chemical Engineering Sikes, Hadley Sikes, Hadley Coupling polymerization initiators to molecular recognition events provide the ability to amplify these events and detect them using the formation of a cross-linked polymer as an inexpensive readout that is visible to the unaided eye. The eosin-tertiary amine co-initiation system, activated by visible light, has proven utility in this context when an average of three eosin molecules are coupled to a protein detection reagent. The present work addresses the question of how detection sensitivity is impacted when the number of eosin molecules per binding event increases in the range of two to fifteen. Unlike in other initiation systems, a non-monotonic relationship is observed between the number of initiators per binding event and the observed detection sensitivity. Burroughs Wellcome Fund (Career Award at the Scientific Interface) Massachusetts Institute of Technology. James H. Ferry Fund for Innovation in Research Education 2015-02-11T18:50:48Z 2015-02-11T18:50:48Z 2014-03 2014-02 Article http://purl.org/eprint/type/JournalArticle 10221336 1521-3927 http://hdl.handle.net/1721.1/94335 Lee, Jungkyu K., and Hadley D. Sikes. “Balancing the Initiation and Molecular Recognition Capabilities of Eosin Macroinitiators of Polymerization-Based Signal Amplification Reactions.” Macromolecular Rapid Communications 35, no. 10 (March 26, 2014): 981–986. https://orcid.org/0000-0002-7096-138X en_US http://dx.doi.org/10.1002/marc.201400035 Macromolecular Rapid Communications Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell Prof. Sikes via Erja Kajosalo |
| spellingShingle | Lee, Jungkyu K. Sikes, Hadley Balancing the Initiation and Molecular Recognition Capabilities of Eosin Macroinitiators of Polymerization-Based Signal Amplification Reactions |
| title | Balancing the Initiation and Molecular Recognition Capabilities of Eosin Macroinitiators of Polymerization-Based Signal Amplification Reactions |
| title_full | Balancing the Initiation and Molecular Recognition Capabilities of Eosin Macroinitiators of Polymerization-Based Signal Amplification Reactions |
| title_fullStr | Balancing the Initiation and Molecular Recognition Capabilities of Eosin Macroinitiators of Polymerization-Based Signal Amplification Reactions |
| title_full_unstemmed | Balancing the Initiation and Molecular Recognition Capabilities of Eosin Macroinitiators of Polymerization-Based Signal Amplification Reactions |
| title_short | Balancing the Initiation and Molecular Recognition Capabilities of Eosin Macroinitiators of Polymerization-Based Signal Amplification Reactions |
| title_sort | balancing the initiation and molecular recognition capabilities of eosin macroinitiators of polymerization based signal amplification reactions |
| url | http://hdl.handle.net/1721.1/94335 https://orcid.org/0000-0002-7096-138X |
| work_keys_str_mv | AT leejungkyuk balancingtheinitiationandmolecularrecognitioncapabilitiesofeosinmacroinitiatorsofpolymerizationbasedsignalamplificationreactions AT sikeshadley balancingtheinitiationandmolecularrecognitioncapabilitiesofeosinmacroinitiatorsofpolymerizationbasedsignalamplificationreactions |