High Field Dynamic Nuclear Polarization NMR with Surfactant Sheltered Biradicals
We illustrate the ability to place a water-insoluble biradical, bTbk, into a glycerol/water matrix with the assistance of a surfactant, sodium octyl sulfate (SOS). This surfactant approach enables a previously water insoluble biradical, bTbk, with favorable electron–electron dipolar coupling to be u...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2015
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| Online Access: | http://hdl.handle.net/1721.1/94614 https://orcid.org/0000-0003-1589-832X https://orcid.org/0000-0002-6708-7660 |
| _version_ | 1826193767875477504 |
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| author | Kiesewetter, Matthew K. Michaelis, Vladimir K. Walish, Joseph John Griffin, Robert Guy Swager, Timothy M |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Kiesewetter, Matthew K. Michaelis, Vladimir K. Walish, Joseph John Griffin, Robert Guy Swager, Timothy M |
| author_sort | Kiesewetter, Matthew K. |
| collection | MIT |
| description | We illustrate the ability to place a water-insoluble biradical, bTbk, into a glycerol/water matrix with the assistance of a surfactant, sodium octyl sulfate (SOS). This surfactant approach enables a previously water insoluble biradical, bTbk, with favorable electron–electron dipolar coupling to be used for dynamic nuclear polarization (DNP) nuclear magnetic resonance (NMR) experiments in frozen, glassy, aqueous media. Nuclear Overhauser enhancement (NOE) and paramagnetic relaxation enhancement (PRE) experiments are conducted to determine the distribution of urea and several biradicals within the SOS macromolecular assembly. We also demonstrate that SOS assemblies are an effective approach by which mixed biradicals are created through an assembly process. |
| first_indexed | 2024-09-23T09:44:35Z |
| format | Article |
| id | mit-1721.1/94614 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:44:35Z |
| publishDate | 2015 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/946142022-09-30T16:32:15Z High Field Dynamic Nuclear Polarization NMR with Surfactant Sheltered Biradicals Kiesewetter, Matthew K. Michaelis, Vladimir K. Walish, Joseph John Griffin, Robert Guy Swager, Timothy M Massachusetts Institute of Technology. Department of Chemistry Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology) Kiesewetter, Matthew K. Michaelis, Vladimir K. Walish, Joseph John Griffin, Robert Guy Swager, Timothy Manning We illustrate the ability to place a water-insoluble biradical, bTbk, into a glycerol/water matrix with the assistance of a surfactant, sodium octyl sulfate (SOS). This surfactant approach enables a previously water insoluble biradical, bTbk, with favorable electron–electron dipolar coupling to be used for dynamic nuclear polarization (DNP) nuclear magnetic resonance (NMR) experiments in frozen, glassy, aqueous media. Nuclear Overhauser enhancement (NOE) and paramagnetic relaxation enhancement (PRE) experiments are conducted to determine the distribution of urea and several biradicals within the SOS macromolecular assembly. We also demonstrate that SOS assemblies are an effective approach by which mixed biradicals are created through an assembly process. National Institutes of Health (U.S.) (Grant EB-002804) National Institutes of Health (U.S.) (Grant EB-002026) National Institutes of Health (U.S.) (Grant EB-001960) National Institutes of Health (U.S.) (Grant GM-095843) Natural Sciences and Engineering Research Council of Canada (Postdoctoral Fellowship) National Institutes of Health. National Institute for Biomedical Imaging and Bioengineering (Ruth L. Kirschstein National Research Service Award F32EB012937) 2015-02-18T20:31:15Z 2015-02-18T20:31:15Z 2014-02 2014-01 Article http://purl.org/eprint/type/JournalArticle 1520-6106 1520-5207 http://hdl.handle.net/1721.1/94614 Kiesewetter, Matthew K., Vladimir K. Michaelis, Joseph J. Walish, Robert G. Griffin, and Timothy M. Swager. “High Field Dynamic Nuclear Polarization NMR with Surfactant Sheltered Biradicals.” The Journal of Physical Chemistry B 118, no. 7 (February 20, 2014): 1825–1830. https://orcid.org/0000-0003-1589-832X https://orcid.org/0000-0002-6708-7660 en_US http://dx.doi.org/10.1021/jp410387e Journal of Physical Chemistry B Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Kiesewetter, Matthew K. Michaelis, Vladimir K. Walish, Joseph John Griffin, Robert Guy Swager, Timothy M High Field Dynamic Nuclear Polarization NMR with Surfactant Sheltered Biradicals |
| title | High Field Dynamic Nuclear Polarization NMR with Surfactant Sheltered Biradicals |
| title_full | High Field Dynamic Nuclear Polarization NMR with Surfactant Sheltered Biradicals |
| title_fullStr | High Field Dynamic Nuclear Polarization NMR with Surfactant Sheltered Biradicals |
| title_full_unstemmed | High Field Dynamic Nuclear Polarization NMR with Surfactant Sheltered Biradicals |
| title_short | High Field Dynamic Nuclear Polarization NMR with Surfactant Sheltered Biradicals |
| title_sort | high field dynamic nuclear polarization nmr with surfactant sheltered biradicals |
| url | http://hdl.handle.net/1721.1/94614 https://orcid.org/0000-0003-1589-832X https://orcid.org/0000-0002-6708-7660 |
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