Effects of Monofunctional Platinum Agents on Bacterial Growth: A Retrospective Study
The effect of the novel and potent monofunctional platinum(II) agent phenanthriplatin on Escherichia coli and bacteriophage λ lysogens is reported. E. coli filamentation was observed by light microscopy when cells were grown in the presence of phenanthriplatin, cis-[Pt(NH[subscript 3])[subscript 2](...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2015
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| Online Access: | http://hdl.handle.net/1721.1/95481 https://orcid.org/0000-0002-2693-4982 |
| _version_ | 1811080794412679168 |
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| author | Alexander, Sarah M. Lin, Wei Lippard, Stephen J. Johnstone, Timothy |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Alexander, Sarah M. Lin, Wei Lippard, Stephen J. Johnstone, Timothy |
| author_sort | Alexander, Sarah M. |
| collection | MIT |
| description | The effect of the novel and potent monofunctional platinum(II) agent phenanthriplatin on Escherichia coli and bacteriophage λ lysogens is reported. E. coli filamentation was observed by light microscopy when cells were grown in the presence of phenanthriplatin, cis-[Pt(NH[subscript 3])[subscript 2](Am)Cl][superscript +] where Am is phenanthridine. Treatment of lysogenic bacteria with this compound resulted in lysis and the production of viral particles, as indicated by plaque formation in a bacterial lawn. The results obtained with phenanthriplatin are contextualized by comparison with those obtained using cisplatin as well as other, less active, monofunctional compounds such as [Pt(NH[subscript 3])[subscript 3]Cl][superscript +] and cis-[Pt(NH3)2(py)Cl]+, where py is pyridine. The ability of phenanthriplatin to induce bacterial filamentation and initiate lysis in lysogenic bacteria corroborates the hypothesis that the biological activity of this complex is mediated by its interaction with DNA. |
| first_indexed | 2024-09-23T11:36:58Z |
| format | Article |
| id | mit-1721.1/95481 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:36:58Z |
| publishDate | 2015 |
| publisher | American Chemical Society (ACS) |
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| spelling | mit-1721.1/954812022-09-27T20:42:36Z Effects of Monofunctional Platinum Agents on Bacterial Growth: A Retrospective Study Alexander, Sarah M. Lin, Wei Lippard, Stephen J. Johnstone, Timothy Massachusetts Institute of Technology. Department of Chemistry Lippard, Stephen J. Johnstone, Timothy Alexander, Sarah M. Lin, Wei The effect of the novel and potent monofunctional platinum(II) agent phenanthriplatin on Escherichia coli and bacteriophage λ lysogens is reported. E. coli filamentation was observed by light microscopy when cells were grown in the presence of phenanthriplatin, cis-[Pt(NH[subscript 3])[subscript 2](Am)Cl][superscript +] where Am is phenanthridine. Treatment of lysogenic bacteria with this compound resulted in lysis and the production of viral particles, as indicated by plaque formation in a bacterial lawn. The results obtained with phenanthriplatin are contextualized by comparison with those obtained using cisplatin as well as other, less active, monofunctional compounds such as [Pt(NH[subscript 3])[subscript 3]Cl][superscript +] and cis-[Pt(NH3)2(py)Cl]+, where py is pyridine. The ability of phenanthriplatin to induce bacterial filamentation and initiate lysis in lysogenic bacteria corroborates the hypothesis that the biological activity of this complex is mediated by its interaction with DNA. National Cancer Institute (U.S.) (Grant CA034992) Massachusetts Institute of Technology. Undergraduate Research Opportunities Program 2015-02-24T16:09:55Z 2015-02-24T16:09:55Z 2013-12 2013-11 Article http://purl.org/eprint/type/JournalArticle 0002-7863 1520-5126 http://hdl.handle.net/1721.1/95481 Johnstone, Timothy C., Sarah M. Alexander, Wei Lin, and Stephen J. Lippard. “Effects of Monofunctional Platinum Agents on Bacterial Growth: A Retrospective Study.” Journal of the American Chemical Society 136, no. 1 (January 8, 2014): 116–118. https://orcid.org/0000-0002-2693-4982 en_US http://dx.doi.org/10.1021/ja411742c Journal of the American Chemical Society Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Alexander, Sarah M. Lin, Wei Lippard, Stephen J. Johnstone, Timothy Effects of Monofunctional Platinum Agents on Bacterial Growth: A Retrospective Study |
| title | Effects of Monofunctional Platinum Agents on Bacterial Growth: A Retrospective Study |
| title_full | Effects of Monofunctional Platinum Agents on Bacterial Growth: A Retrospective Study |
| title_fullStr | Effects of Monofunctional Platinum Agents on Bacterial Growth: A Retrospective Study |
| title_full_unstemmed | Effects of Monofunctional Platinum Agents on Bacterial Growth: A Retrospective Study |
| title_short | Effects of Monofunctional Platinum Agents on Bacterial Growth: A Retrospective Study |
| title_sort | effects of monofunctional platinum agents on bacterial growth a retrospective study |
| url | http://hdl.handle.net/1721.1/95481 https://orcid.org/0000-0002-2693-4982 |
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