Enzymatic “Click” Ligation: Selective Cysteine Modification in Polypeptides Enabled by Promiscuous Glutathione S-Transferase
Singled out for special treatment: Naturally occurring glutathione S-transferase (GST) was used to catalyze an efficient “click” ligation between polypeptides with an N-terminal glutathione sequence and biomolecules or chemical probes containing perfluorinated aromatic groups (see scheme). The site-...
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2015
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| Online Access: | http://hdl.handle.net/1721.1/95629 https://orcid.org/0000-0001-9519-7456 https://orcid.org/0000-0003-1632-5195 |
| _version_ | 1826215595104796672 |
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| author | Zhang, Chi Spokoyny, Alexander M. Zou, Yekui Pentelute, Bradley L. Simon, Mark |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Zhang, Chi Spokoyny, Alexander M. Zou, Yekui Pentelute, Bradley L. Simon, Mark |
| author_sort | Zhang, Chi |
| collection | MIT |
| description | Singled out for special treatment: Naturally occurring glutathione S-transferase (GST) was used to catalyze an efficient “click” ligation between polypeptides with an N-terminal glutathione sequence and biomolecules or chemical probes containing perfluorinated aromatic groups (see scheme). The site-specific modification of one cysteine residue was possible in the presence of other unprotected cysteine residues and reactive functional groups. |
| first_indexed | 2024-09-23T16:36:23Z |
| format | Article |
| id | mit-1721.1/95629 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:36:23Z |
| publishDate | 2015 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/956292022-10-02T08:24:39Z Enzymatic “Click” Ligation: Selective Cysteine Modification in Polypeptides Enabled by Promiscuous Glutathione S-Transferase Zhang, Chi Spokoyny, Alexander M. Zou, Yekui Pentelute, Bradley L. Simon, Mark Massachusetts Institute of Technology. Department of Chemistry Zhang, Chi Spokoyny, Alexander M. Zou, Yekui Simon, Mark Pentelute, Bradley L. Singled out for special treatment: Naturally occurring glutathione S-transferase (GST) was used to catalyze an efficient “click” ligation between polypeptides with an N-terminal glutathione sequence and biomolecules or chemical probes containing perfluorinated aromatic groups (see scheme). The site-specific modification of one cysteine residue was possible in the presence of other unprotected cysteine residues and reactive functional groups. National Institutes of Health (U.S.) (GM101762) National Institutes of Health (U.S.) (Award GM46059) MIT Faculty Start-up Fund Damon Runyon Cancer Research Foundation Amgen Inc. (Summer Graduate Research Fellowship) 2015-02-25T20:15:29Z 2015-02-25T20:15:29Z 2013-11 2013-09 Article http://purl.org/eprint/type/JournalArticle 14337851 1521-3773 http://hdl.handle.net/1721.1/95629 Zhang, Chi, Alexander M. Spokoyny, Yekui Zou, Mark D. Simon, and Bradley L. Pentelute. “Enzymatic ‘Click’ Ligation: Selective Cysteine Modification in Polypeptides Enabled by Promiscuous Glutathione S-Transferase.” Angewandte Chemie International Edition 52, no. 52 (November 12, 2013): 14001–14005. https://orcid.org/0000-0001-9519-7456 https://orcid.org/0000-0003-1632-5195 en_US http://dx.doi.org/10.1002/anie.201306430 Angewandte Chemie International Edition Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Zhang, Chi Spokoyny, Alexander M. Zou, Yekui Pentelute, Bradley L. Simon, Mark Enzymatic “Click” Ligation: Selective Cysteine Modification in Polypeptides Enabled by Promiscuous Glutathione S-Transferase |
| title | Enzymatic “Click” Ligation: Selective Cysteine Modification in Polypeptides Enabled by Promiscuous Glutathione S-Transferase |
| title_full | Enzymatic “Click” Ligation: Selective Cysteine Modification in Polypeptides Enabled by Promiscuous Glutathione S-Transferase |
| title_fullStr | Enzymatic “Click” Ligation: Selective Cysteine Modification in Polypeptides Enabled by Promiscuous Glutathione S-Transferase |
| title_full_unstemmed | Enzymatic “Click” Ligation: Selective Cysteine Modification in Polypeptides Enabled by Promiscuous Glutathione S-Transferase |
| title_short | Enzymatic “Click” Ligation: Selective Cysteine Modification in Polypeptides Enabled by Promiscuous Glutathione S-Transferase |
| title_sort | enzymatic click ligation selective cysteine modification in polypeptides enabled by promiscuous glutathione s transferase |
| url | http://hdl.handle.net/1721.1/95629 https://orcid.org/0000-0001-9519-7456 https://orcid.org/0000-0003-1632-5195 |
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