Characterization of Particle Translocation through Mucin Hydrogels
Biological functional entities surround themselves with selective barriers that control the passage of certain classes of macromolecules while rejecting others. A prominent example of such a selective permeability barrier is given by mucus. Mucus is a biopolymer-based hydrogel that lines all wet epi...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2015
|
| Online Access: | http://hdl.handle.net/1721.1/96055 https://orcid.org/0000-0001-8260-338X |
| _version_ | 1826191859188236288 |
|---|---|
| author | Lieleg, Oliver Vladescu, Ioana Ribbeck, Katharina |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Lieleg, Oliver Vladescu, Ioana Ribbeck, Katharina |
| author_sort | Lieleg, Oliver |
| collection | MIT |
| description | Biological functional entities surround themselves with selective barriers that control the passage of certain classes of macromolecules while rejecting others. A prominent example of such a selective permeability barrier is given by mucus. Mucus is a biopolymer-based hydrogel that lines all wet epithelial surfaces of the human body. It regulates the uptake of nutrients from our gastrointestinal system, adjusts itself with the menstrual cycle to control the passage of sperm, and shields the underlying cells from pathogens such as bacteria and viruses. In the case of drug delivery, the mucus barrier needs to be overcome for successful medical treatment. Despite its importance for both physiology and medical applications, the underlying principles which regulate the permeability of mucus remain enigmatic. Here, we analyze the mobility of microscopic particles in reconstituted mucin hydrogels. We show that electrostatic interactions between diffusing particles and mucin polymers regulate the permeability properties of reconstituted mucin hydrogels. As a consequence, various parameters such as particle surface charge and mucin density, and buffer conditions such as pH and ionic strength, can modulate the microscopic barrier function of the mucin hydrogel. Our findings suggest that the permeability of a biopolymer-based hydrogel such as native mucus can be tuned to a wide range of settings in different compartments of our bodies. |
| first_indexed | 2024-09-23T09:02:23Z |
| format | Article |
| id | mit-1721.1/96055 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:02:23Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/960552022-09-30T12:58:13Z Characterization of Particle Translocation through Mucin Hydrogels Lieleg, Oliver Vladescu, Ioana Ribbeck, Katharina Massachusetts Institute of Technology. Department of Biological Engineering Ribbeck, Katharina Lieleg, Oliver Vladescu, Ioana Biological functional entities surround themselves with selective barriers that control the passage of certain classes of macromolecules while rejecting others. A prominent example of such a selective permeability barrier is given by mucus. Mucus is a biopolymer-based hydrogel that lines all wet epithelial surfaces of the human body. It regulates the uptake of nutrients from our gastrointestinal system, adjusts itself with the menstrual cycle to control the passage of sperm, and shields the underlying cells from pathogens such as bacteria and viruses. In the case of drug delivery, the mucus barrier needs to be overcome for successful medical treatment. Despite its importance for both physiology and medical applications, the underlying principles which regulate the permeability of mucus remain enigmatic. Here, we analyze the mobility of microscopic particles in reconstituted mucin hydrogels. We show that electrostatic interactions between diffusing particles and mucin polymers regulate the permeability properties of reconstituted mucin hydrogels. As a consequence, various parameters such as particle surface charge and mucin density, and buffer conditions such as pH and ionic strength, can modulate the microscopic barrier function of the mucin hydrogel. Our findings suggest that the permeability of a biopolymer-based hydrogel such as native mucus can be tuned to a wide range of settings in different compartments of our bodies. German Academic Exchange Service (Postdoctoral Fellowship) National Institutes of Health (U.S.) (P50GM068763) 2015-03-17T19:00:44Z 2015-03-17T19:00:44Z 2010-05 2009-11 Article http://purl.org/eprint/type/JournalArticle 00063495 1542-0086 http://hdl.handle.net/1721.1/96055 Lieleg, Oliver, Ioana Vladescu, and Katharina Ribbeck. “Characterization of Particle Translocation through Mucin Hydrogels.” Biophysical Journal 98, no. 9 (May 2010): 1782–1789. © 2010 Biophysical Society https://orcid.org/0000-0001-8260-338X en_US http://dx.doi.org/10.1016/j.bpj.2010.01.012 Biophysical Journal Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier |
| spellingShingle | Lieleg, Oliver Vladescu, Ioana Ribbeck, Katharina Characterization of Particle Translocation through Mucin Hydrogels |
| title | Characterization of Particle Translocation through Mucin Hydrogels |
| title_full | Characterization of Particle Translocation through Mucin Hydrogels |
| title_fullStr | Characterization of Particle Translocation through Mucin Hydrogels |
| title_full_unstemmed | Characterization of Particle Translocation through Mucin Hydrogels |
| title_short | Characterization of Particle Translocation through Mucin Hydrogels |
| title_sort | characterization of particle translocation through mucin hydrogels |
| url | http://hdl.handle.net/1721.1/96055 https://orcid.org/0000-0001-8260-338X |
| work_keys_str_mv | AT lielegoliver characterizationofparticletranslocationthroughmucinhydrogels AT vladescuioana characterizationofparticletranslocationthroughmucinhydrogels AT ribbeckkatharina characterizationofparticletranslocationthroughmucinhydrogels |