BBF RFC 106: A Standard Type IIS Syntax for Plants
Here we define a standard syntax for assembling standard parts for expression in plant cells, extensible to all other eukaryotes. Variations of the Type IIS mediated cloning method known as Golden Gate Cloning, most notably Golden Braid (GB2.0) and Golden Gate Modular Cloning (MoClo) are in common u...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Technical Report |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/96069 |
| _version_ | 1811085147652489216 |
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| author | Rutten, Virginia Munabi, Angelina Riche, Fergus Lewy, Guy Wilson, Hugh Pipan, Miha Bhate, Salil Nghiem, Trang-Anh Kaufhold, Will Haseloff, Jim Rubert, Alba González, Alejandra Quijano, Alfredo Llopis, Ivan Gavaldá, Jose Estellés, Lucía Vásquez, Marta Orzáez, Diego Deal, Cara Gray, Jessica Spiegel, Mischa Monsey, Steven Middlemiss, Alistair Day, Jack Patron, Nicola |
| author_facet | Rutten, Virginia Munabi, Angelina Riche, Fergus Lewy, Guy Wilson, Hugh Pipan, Miha Bhate, Salil Nghiem, Trang-Anh Kaufhold, Will Haseloff, Jim Rubert, Alba González, Alejandra Quijano, Alfredo Llopis, Ivan Gavaldá, Jose Estellés, Lucía Vásquez, Marta Orzáez, Diego Deal, Cara Gray, Jessica Spiegel, Mischa Monsey, Steven Middlemiss, Alistair Day, Jack Patron, Nicola |
| author_sort | Rutten, Virginia |
| collection | MIT |
| description | Here we define a standard syntax for assembling standard parts for expression in plant cells, extensible to all other eukaryotes. Variations of the Type IIS mediated cloning method known as Golden Gate Cloning, most notably Golden Braid (GB2.0) and Golden Gate Modular Cloning (MoClo) are in common use, particularly for the assembly of plasmids for delivery to plant cells. Many characterised plant parts compatible with Type IIS mediated assembly are available outside of the Registry of Standard Parts, as well as plasmids with the features necessary for delivery of DNA to plants cells via the shuttle chassis, Agrobacterium tumefaciens. This RFC describes a consensus Type IIS syntax for plant parts to allow assembly into complete eukaryotic transcriptional units in plasmid vectors that contain the necessary features for transfection of plant chassis. We use Marchantia polymorpha, a primitive and easy-to-engineer liverwort and Nicotiana benthamiana a model plant as exemplar chassis. |
| first_indexed | 2024-09-23T13:03:37Z |
| format | Technical Report |
| id | mit-1721.1/96069 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T13:03:37Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | mit-1721.1/960692019-04-12T14:13:28Z BBF RFC 106: A Standard Type IIS Syntax for Plants Rutten, Virginia Munabi, Angelina Riche, Fergus Lewy, Guy Wilson, Hugh Pipan, Miha Bhate, Salil Nghiem, Trang-Anh Kaufhold, Will Haseloff, Jim Rubert, Alba González, Alejandra Quijano, Alfredo Llopis, Ivan Gavaldá, Jose Estellés, Lucía Vásquez, Marta Orzáez, Diego Deal, Cara Gray, Jessica Spiegel, Mischa Monsey, Steven Middlemiss, Alistair Day, Jack Patron, Nicola assembly standard plant Here we define a standard syntax for assembling standard parts for expression in plant cells, extensible to all other eukaryotes. Variations of the Type IIS mediated cloning method known as Golden Gate Cloning, most notably Golden Braid (GB2.0) and Golden Gate Modular Cloning (MoClo) are in common use, particularly for the assembly of plasmids for delivery to plant cells. Many characterised plant parts compatible with Type IIS mediated assembly are available outside of the Registry of Standard Parts, as well as plasmids with the features necessary for delivery of DNA to plants cells via the shuttle chassis, Agrobacterium tumefaciens. This RFC describes a consensus Type IIS syntax for plant parts to allow assembly into complete eukaryotic transcriptional units in plasmid vectors that contain the necessary features for transfection of plant chassis. We use Marchantia polymorpha, a primitive and easy-to-engineer liverwort and Nicotiana benthamiana a model plant as exemplar chassis. 2015-03-18T01:25:23Z 2015-03-18T01:25:23Z 2015-03-17 Technical Report http://hdl.handle.net/1721.1/96069 en BBF RFC;106 Attribution-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nd/3.0/us/ application/pdf |
| spellingShingle | assembly standard plant Rutten, Virginia Munabi, Angelina Riche, Fergus Lewy, Guy Wilson, Hugh Pipan, Miha Bhate, Salil Nghiem, Trang-Anh Kaufhold, Will Haseloff, Jim Rubert, Alba González, Alejandra Quijano, Alfredo Llopis, Ivan Gavaldá, Jose Estellés, Lucía Vásquez, Marta Orzáez, Diego Deal, Cara Gray, Jessica Spiegel, Mischa Monsey, Steven Middlemiss, Alistair Day, Jack Patron, Nicola BBF RFC 106: A Standard Type IIS Syntax for Plants |
| title | BBF RFC 106: A Standard Type IIS Syntax for Plants |
| title_full | BBF RFC 106: A Standard Type IIS Syntax for Plants |
| title_fullStr | BBF RFC 106: A Standard Type IIS Syntax for Plants |
| title_full_unstemmed | BBF RFC 106: A Standard Type IIS Syntax for Plants |
| title_short | BBF RFC 106: A Standard Type IIS Syntax for Plants |
| title_sort | bbf rfc 106 a standard type iis syntax for plants |
| topic | assembly standard plant |
| url | http://hdl.handle.net/1721.1/96069 |
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