Cell Polarity Determinants Establish Asymmetry in MEN Signaling
Components of the mitotic exit network (MEN), a signaling pathway that triggers exit from mitosis, localize to the spindle pole body (SPB) that migrates into the daughter cell during anaphase but are largely absent from the SPB that remains in the mother cell. Through the analysis of one of the dete...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier B.V.
2015
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| Online Access: | http://hdl.handle.net/1721.1/96143 https://orcid.org/0000-0001-9837-0314 |
| _version_ | 1826201547242995712 |
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| author | Monje-Casas, Fernando Amon, Angelika B |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Monje-Casas, Fernando Amon, Angelika B |
| author_sort | Monje-Casas, Fernando |
| collection | MIT |
| description | Components of the mitotic exit network (MEN), a signaling pathway that triggers exit from mitosis, localize to the spindle pole body (SPB) that migrates into the daughter cell during anaphase but are largely absent from the SPB that remains in the mother cell. Through the analysis of one of the determinants of this asymmetry, Bfa1, we find that the machinery responsible for establishing cell polarity and cytoplasmic microtubules collaborate to establish MEN asymmetry. In cells defective in the Cdc42 signaling pathway or the formin Bni1, Bfa1 localizes to both SPBs. The quantitative analysis of Bfa1 localization further shows that Bfa1 can associate with both SPBs in a transient and highly dynamic fashion, but the protein is stabilized on the SPB that migrates into the daughter cell during anaphase through microtubule-bud cortex interactions. Our results indicate that mother-daughter cell asymmetry determinants establish MEN signaling asymmetry through microtubule-bud cortex interactions. |
| first_indexed | 2024-09-23T11:53:27Z |
| format | Article |
| id | mit-1721.1/96143 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:53:27Z |
| publishDate | 2015 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | mit-1721.1/961432022-09-27T22:36:52Z Cell Polarity Determinants Establish Asymmetry in MEN Signaling Monje-Casas, Fernando Amon, Angelika B Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Monje-Casas, Fernando Amon, Angelika B. Components of the mitotic exit network (MEN), a signaling pathway that triggers exit from mitosis, localize to the spindle pole body (SPB) that migrates into the daughter cell during anaphase but are largely absent from the SPB that remains in the mother cell. Through the analysis of one of the determinants of this asymmetry, Bfa1, we find that the machinery responsible for establishing cell polarity and cytoplasmic microtubules collaborate to establish MEN asymmetry. In cells defective in the Cdc42 signaling pathway or the formin Bni1, Bfa1 localizes to both SPBs. The quantitative analysis of Bfa1 localization further shows that Bfa1 can associate with both SPBs in a transient and highly dynamic fashion, but the protein is stabilized on the SPB that migrates into the daughter cell during anaphase through microtubule-bud cortex interactions. Our results indicate that mother-daughter cell asymmetry determinants establish MEN signaling asymmetry through microtubule-bud cortex interactions. Howard Hughes Medical Institute (Investigator) Massachusetts Institute of Technology (Charles A. King Trust Postdoctoral Research Fellowship Program) National Institutes of Health (U.S.) (GM056800) 2015-03-20T19:32:43Z 2015-03-20T19:32:43Z 2009-01 2008-09 Article http://purl.org/eprint/type/JournalArticle 15345807 http://hdl.handle.net/1721.1/96143 Monje-Casas, Fernando, and Angelika Amon. “Cell Polarity Determinants Establish Asymmetry in MEN Signaling.” Developmental Cell 16, no. 1 (January 2009): 132–145. © 2009 Elsevier Inc. https://orcid.org/0000-0001-9837-0314 en_US http://dx.doi.org/10.1016/j.devcel.2008.11.002 Developmental Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier B.V. Elsevier |
| spellingShingle | Monje-Casas, Fernando Amon, Angelika B Cell Polarity Determinants Establish Asymmetry in MEN Signaling |
| title | Cell Polarity Determinants Establish Asymmetry in MEN Signaling |
| title_full | Cell Polarity Determinants Establish Asymmetry in MEN Signaling |
| title_fullStr | Cell Polarity Determinants Establish Asymmetry in MEN Signaling |
| title_full_unstemmed | Cell Polarity Determinants Establish Asymmetry in MEN Signaling |
| title_short | Cell Polarity Determinants Establish Asymmetry in MEN Signaling |
| title_sort | cell polarity determinants establish asymmetry in men signaling |
| url | http://hdl.handle.net/1721.1/96143 https://orcid.org/0000-0001-9837-0314 |
| work_keys_str_mv | AT monjecasasfernando cellpolaritydeterminantsestablishasymmetryinmensignaling AT amonangelikab cellpolaritydeterminantsestablishasymmetryinmensignaling |