Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease
Reprogramming of human somatic cells uses readily accessible tissue, such as skin or blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This procedure has been applied to somatic cells from patients who are classified into a disease group, thus creating “disease-specific” iPSC...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2015
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| Online Access: | http://hdl.handle.net/1721.1/96182 |
| _version_ | 1811097707827167232 |
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| author | Saha, Krishanu Jaenisch, Rudolf |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Saha, Krishanu Jaenisch, Rudolf |
| author_sort | Saha, Krishanu |
| collection | MIT |
| description | Reprogramming of human somatic cells uses readily accessible tissue, such as skin or blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This procedure has been applied to somatic cells from patients who are classified into a disease group, thus creating “disease-specific” iPSCs. Here, we examine the challenges and assumptions in creating a disease model from a single cell of the patient. Both the kinetics of disease onset and progression as well as the spatial localization of disease in the patient's body are challenges to disease modeling. New tools in genetic modification, reprogramming, biomaterials, and animal models can be used for addressing these challenges. |
| first_indexed | 2024-09-23T17:03:39Z |
| format | Article |
| id | mit-1721.1/96182 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T17:03:39Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/961822022-09-29T23:23:18Z Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease Saha, Krishanu Jaenisch, Rudolf Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Jaenisch, Rudolf Reprogramming of human somatic cells uses readily accessible tissue, such as skin or blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This procedure has been applied to somatic cells from patients who are classified into a disease group, thus creating “disease-specific” iPSCs. Here, we examine the challenges and assumptions in creating a disease model from a single cell of the patient. Both the kinetics of disease onset and progression as well as the spatial localization of disease in the patient's body are challenges to disease modeling. New tools in genetic modification, reprogramming, biomaterials, and animal models can be used for addressing these challenges. National Institutes of Health (U.S.) (Grant RO1-HD045022) National Institutes of Health (U.S.) (Grant R37-CA084198) National Institutes of Health (U.S.). (Grant RO1-CA087869) 2015-03-25T16:51:24Z 2015-03-25T16:51:24Z 2009-12 Article http://purl.org/eprint/type/JournalArticle 19345909 http://hdl.handle.net/1721.1/96182 Saha, Krishanu, and Rudolf Jaenisch. “Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease.” Cell Stem Cell 5, no. 6 (December 2009): 584–595. © 2009 Elsevier Inc. en_US http://dx.doi.org/10.1016/j.stem.2009.11.009 Cell Stem Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier |
| spellingShingle | Saha, Krishanu Jaenisch, Rudolf Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease |
| title | Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease |
| title_full | Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease |
| title_fullStr | Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease |
| title_full_unstemmed | Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease |
| title_short | Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease |
| title_sort | technical challenges in using human induced pluripotent stem cells to model disease |
| url | http://hdl.handle.net/1721.1/96182 |
| work_keys_str_mv | AT sahakrishanu technicalchallengesinusinghumaninducedpluripotentstemcellstomodeldisease AT jaenischrudolf technicalchallengesinusinghumaninducedpluripotentstemcellstomodeldisease |