Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition
The oocyte-to-embryo transition marks the onset of development. The initial phase of this profound change from the differentiated oocyte to the totipotent embryo occurs in the absence of both transcription and mRNA degradation. Here we combine global polysome profiling, ribosome-footprint profiling,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2015
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| Online Access: | http://hdl.handle.net/1721.1/96184 https://orcid.org/0000-0002-6410-4699 https://orcid.org/0000-0002-3872-2856 https://orcid.org/0000-0002-7934-111X |
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| author | Kronja, Iva Yuan, Bingbing Dzeyk, Kristina Krijgsveld, Jeroen Eichhorn, Stephen William Bartel, David Orr-Weaver, Terry L. Orr-Weaver, Terry |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Kronja, Iva Yuan, Bingbing Dzeyk, Kristina Krijgsveld, Jeroen Eichhorn, Stephen William Bartel, David Orr-Weaver, Terry L. Orr-Weaver, Terry |
| author_sort | Kronja, Iva |
| collection | MIT |
| description | The oocyte-to-embryo transition marks the onset of development. The initial phase of this profound change from the differentiated oocyte to the totipotent embryo occurs in the absence of both transcription and mRNA degradation. Here we combine global polysome profiling, ribosome-footprint profiling, and quantitative mass spectrometry in a comprehensive approach to delineate the translational and proteomic changes that occur during this important transition in Drosophila. Our results show that PNG kinase is a critical regulator of the extensive changes in the translatome, acting uniquely at this developmental window. Analysis of the proteome in png mutants provided insights into the contributions of translation to changes in protein levels, revealing a compensatory dynamic between translation and protein turnover during proteome remodeling at the return to totipotency. The proteome changes additionally suggested regulators of meiosis and early embryogenesis, including the conserved H3K4 demethylase LID, which we demonstrated is required during this period despite transcriptional inactivity. |
| first_indexed | 2024-09-23T12:38:31Z |
| format | Article |
| id | mit-1721.1/96184 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:38:31Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/961842022-09-28T09:07:25Z Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition Kronja, Iva Yuan, Bingbing Dzeyk, Kristina Krijgsveld, Jeroen Eichhorn, Stephen William Bartel, David Orr-Weaver, Terry L. Orr-Weaver, Terry Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Eichhorn, Stephen William Bartel, David Orr-Weaver, Terry L. The oocyte-to-embryo transition marks the onset of development. The initial phase of this profound change from the differentiated oocyte to the totipotent embryo occurs in the absence of both transcription and mRNA degradation. Here we combine global polysome profiling, ribosome-footprint profiling, and quantitative mass spectrometry in a comprehensive approach to delineate the translational and proteomic changes that occur during this important transition in Drosophila. Our results show that PNG kinase is a critical regulator of the extensive changes in the translatome, acting uniquely at this developmental window. Analysis of the proteome in png mutants provided insights into the contributions of translation to changes in protein levels, revealing a compensatory dynamic between translation and protein turnover during proteome remodeling at the return to totipotency. The proteome changes additionally suggested regulators of meiosis and early embryogenesis, including the conserved H3K4 demethylase LID, which we demonstrated is required during this period despite transcriptional inactivity. National Institutes of Health (U.S.) (Grant GM39341) 2015-03-25T17:01:01Z 2015-03-25T17:01:01Z 2014-05 2014-03 Article http://purl.org/eprint/type/JournalArticle 22111247 http://hdl.handle.net/1721.1/96184 Kronja, Iva, Bingbing Yuan, Stephen W. Eichhorn, Kristina Dzeyk, Jeroen Krijgsveld, David P. Bartel, and Terry L. Orr-Weaver. “Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition.” Cell Reports 7, no. 5 (June 2014): 1495–1508. https://orcid.org/0000-0002-6410-4699 https://orcid.org/0000-0002-3872-2856 https://orcid.org/0000-0002-7934-111X en_US http://dx.doi.org/10.1016/j.celrep.2014.05.002 Cell Reports Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported http://creativecommons.org/licenses/by-nc-nd/3.0/ application/pdf Elsevier Elsevier Open Access |
| spellingShingle | Kronja, Iva Yuan, Bingbing Dzeyk, Kristina Krijgsveld, Jeroen Eichhorn, Stephen William Bartel, David Orr-Weaver, Terry L. Orr-Weaver, Terry Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition |
| title | Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition |
| title_full | Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition |
| title_fullStr | Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition |
| title_full_unstemmed | Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition |
| title_short | Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition |
| title_sort | widespread changes in the posttranscriptional landscape at the drosophila oocyte to embryo transition |
| url | http://hdl.handle.net/1721.1/96184 https://orcid.org/0000-0002-6410-4699 https://orcid.org/0000-0002-3872-2856 https://orcid.org/0000-0002-7934-111X |
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