Changes in Cell Morphology Are Coordinated with Cell Growth through the TORC1 Pathway
Background: Growth rate is determined not only by extracellular cues such as nutrient availability but also by intracellular processes. Changes in cell morphology in budding yeast, mediated by polarization of the actin cytoskeleton, have been shown to reduce cell growth. Results: Here we demonstr...
| Main Authors: | , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2015
|
| Online Access: | http://hdl.handle.net/1721.1/96218 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0001-9837-0314 |
| _version_ | 1826218167962173440 |
|---|---|
| author | Goranov, Alexi I. Gulati, Amneet Dephoure, Noah Takahara, Terunao Maeda, Tatsuya Gygi, Steven P. Manalis, Scott R Amon, Angelika B |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Goranov, Alexi I. Gulati, Amneet Dephoure, Noah Takahara, Terunao Maeda, Tatsuya Gygi, Steven P. Manalis, Scott R Amon, Angelika B |
| author_sort | Goranov, Alexi I. |
| collection | MIT |
| description | Background:
Growth rate is determined not only by extracellular cues such as nutrient availability but also by intracellular processes. Changes in cell morphology in budding yeast, mediated by polarization of the actin cytoskeleton, have been shown to reduce cell growth.
Results:
Here we demonstrate that polarization of the actin cytoskeleton inhibits the highly conserved Target of Rapamycin Complex 1 (TORC1) pathway. This downregulation is suppressed by inactivation of the TORC1 pathway regulatory Iml1 complex, which also regulates TORC1 during nitrogen starvation. We further demonstrate that attenuation of growth is important for cell recovery after conditions of prolonged polarized growth.
Conclusions:
Our results indicate that extended periods of polarized growth inhibit protein synthesis, mass accumulation, and the increase in cell size at least in part through inhibiting the TORC1 pathway. We speculate that this mechanism serves to coordinate the ability of cells to increase in size with their biosynthetic capacity. |
| first_indexed | 2024-09-23T17:15:01Z |
| format | Article |
| id | mit-1721.1/96218 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T17:15:01Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/962182022-09-30T00:42:28Z Changes in Cell Morphology Are Coordinated with Cell Growth through the TORC1 Pathway Goranov, Alexi I. Gulati, Amneet Dephoure, Noah Takahara, Terunao Maeda, Tatsuya Gygi, Steven P. Manalis, Scott R Amon, Angelika B Massachusetts Institute of Technology. Department of Biological Engineering Koch Institute for Integrative Cancer Research at MIT Goranov, Alexi I. Gulati, Amneet Manalis, Scott R. Amon, Angelika B. Background: Growth rate is determined not only by extracellular cues such as nutrient availability but also by intracellular processes. Changes in cell morphology in budding yeast, mediated by polarization of the actin cytoskeleton, have been shown to reduce cell growth. Results: Here we demonstrate that polarization of the actin cytoskeleton inhibits the highly conserved Target of Rapamycin Complex 1 (TORC1) pathway. This downregulation is suppressed by inactivation of the TORC1 pathway regulatory Iml1 complex, which also regulates TORC1 during nitrogen starvation. We further demonstrate that attenuation of growth is important for cell recovery after conditions of prolonged polarized growth. Conclusions: Our results indicate that extended periods of polarized growth inhibit protein synthesis, mass accumulation, and the increase in cell size at least in part through inhibiting the TORC1 pathway. We speculate that this mechanism serves to coordinate the ability of cells to increase in size with their biosynthetic capacity. American Cancer Society (Postdoctoral Fellowship) National Cancer Institute (U.S.). Physical Sciences Oncology Center (Contract U54CA143874) 2015-03-27T16:34:22Z 2015-03-27T16:34:22Z 2013-06 2013-04 Article http://purl.org/eprint/type/JournalArticle 09609822 1879-0445 http://hdl.handle.net/1721.1/96218 Goranov, Alexi I., Amneet Gulati, Noah Dephoure, Terunao Takahara, Tatsuya Maeda, Steven P. Gygi, Scott Manalis, and Angelika Amon. “Changes in Cell Morphology Are Coordinated with Cell Growth through the TORC1 Pathway.” Current Biology 23, no. 14 (July 2013): 1269–1279. © 2013 Elsevier Ltd. https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0001-9837-0314 en_US http://dx.doi.org/10.1016/j.cub.2013.05.035 Current Biology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier Open Archive |
| spellingShingle | Goranov, Alexi I. Gulati, Amneet Dephoure, Noah Takahara, Terunao Maeda, Tatsuya Gygi, Steven P. Manalis, Scott R Amon, Angelika B Changes in Cell Morphology Are Coordinated with Cell Growth through the TORC1 Pathway |
| title | Changes in Cell Morphology Are Coordinated with Cell Growth through the TORC1 Pathway |
| title_full | Changes in Cell Morphology Are Coordinated with Cell Growth through the TORC1 Pathway |
| title_fullStr | Changes in Cell Morphology Are Coordinated with Cell Growth through the TORC1 Pathway |
| title_full_unstemmed | Changes in Cell Morphology Are Coordinated with Cell Growth through the TORC1 Pathway |
| title_short | Changes in Cell Morphology Are Coordinated with Cell Growth through the TORC1 Pathway |
| title_sort | changes in cell morphology are coordinated with cell growth through the torc1 pathway |
| url | http://hdl.handle.net/1721.1/96218 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0001-9837-0314 |
| work_keys_str_mv | AT goranovalexii changesincellmorphologyarecoordinatedwithcellgrowththroughthetorc1pathway AT gulatiamneet changesincellmorphologyarecoordinatedwithcellgrowththroughthetorc1pathway AT dephourenoah changesincellmorphologyarecoordinatedwithcellgrowththroughthetorc1pathway AT takaharaterunao changesincellmorphologyarecoordinatedwithcellgrowththroughthetorc1pathway AT maedatatsuya changesincellmorphologyarecoordinatedwithcellgrowththroughthetorc1pathway AT gygistevenp changesincellmorphologyarecoordinatedwithcellgrowththroughthetorc1pathway AT manalisscottr changesincellmorphologyarecoordinatedwithcellgrowththroughthetorc1pathway AT amonangelikab changesincellmorphologyarecoordinatedwithcellgrowththroughthetorc1pathway |