Deep, noninvasive imaging and surgical guidance of submillimeter tumors using targeted M13-stabilized single-walled carbon nanotubes
Highly sensitive detection of small, deep tumors for early diagnosis and surgical interventions remains a challenge for conventional imaging modalities. Second-window near-infrared light (NIR2, 950–1,400 nm) is promising for in vivo fluorescence imaging due to deep tissue penetration and low tissue...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2015
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| Online Access: | http://hdl.handle.net/1721.1/96311 https://orcid.org/0000-0001-9353-7453 https://orcid.org/0000-0002-0930-302X https://orcid.org/0000-0002-1293-2097 |
| _version_ | 1811081990897664000 |
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| author | Ghosh, Debadyuti Na, Young Jeong Birrer, Michael J. Bagley, Alexander F Bhatia, Sangeeta N Belcher, Angela M |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Ghosh, Debadyuti Na, Young Jeong Birrer, Michael J. Bagley, Alexander F Bhatia, Sangeeta N Belcher, Angela M |
| author_sort | Ghosh, Debadyuti |
| collection | MIT |
| description | Highly sensitive detection of small, deep tumors for early diagnosis and surgical interventions remains a challenge for conventional imaging modalities. Second-window near-infrared light (NIR2, 950–1,400 nm) is promising for in vivo fluorescence imaging due to deep tissue penetration and low tissue autofluorescence. With their intrinsic fluorescence in the NIR2 regime and lack of photobleaching, single-walled carbon nanotubes (SWNTs) are potentially attractive contrast agents to detect tumors. Here, targeted M13 virus-stabilized SWNTs are used to visualize deep, disseminated tumors in vivo. This targeted nanoprobe, which uses M13 to stably display both tumor-targeting peptides and an SWNT imaging probe, demonstrates excellent tumor-to-background uptake and exhibits higher signal-to-noise performance compared with visible and near-infrared (NIR1) dyes for delineating tumor nodules. Detection and excision of tumors by a gynecological surgeon improved with SWNT image guidance and led to the identification of submillimeter tumors. Collectively, these findings demonstrate the promise of targeted SWNT nanoprobes for noninvasive disease monitoring and guided surgery. |
| first_indexed | 2024-09-23T11:55:20Z |
| format | Article |
| id | mit-1721.1/96311 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:55:20Z |
| publishDate | 2015 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/963112022-09-27T22:51:41Z Deep, noninvasive imaging and surgical guidance of submillimeter tumors using targeted M13-stabilized single-walled carbon nanotubes Ghosh, Debadyuti Na, Young Jeong Birrer, Michael J. Bagley, Alexander F Bhatia, Sangeeta N Belcher, Angela M Massachusetts Institute of Technology. Institute for Medical Engineering & Science Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Materials Science and Engineering Koch Institute for Integrative Cancer Research at MIT Ghosh, Debadyuti Bagley, Alexander F. Bhatia, Sangeeta N. Belcher, Angela M. Highly sensitive detection of small, deep tumors for early diagnosis and surgical interventions remains a challenge for conventional imaging modalities. Second-window near-infrared light (NIR2, 950–1,400 nm) is promising for in vivo fluorescence imaging due to deep tissue penetration and low tissue autofluorescence. With their intrinsic fluorescence in the NIR2 regime and lack of photobleaching, single-walled carbon nanotubes (SWNTs) are potentially attractive contrast agents to detect tumors. Here, targeted M13 virus-stabilized SWNTs are used to visualize deep, disseminated tumors in vivo. This targeted nanoprobe, which uses M13 to stably display both tumor-targeting peptides and an SWNT imaging probe, demonstrates excellent tumor-to-background uptake and exhibits higher signal-to-noise performance compared with visible and near-infrared (NIR1) dyes for delineating tumor nodules. Detection and excision of tumors by a gynecological surgeon improved with SWNT image guidance and led to the identification of submillimeter tumors. Collectively, these findings demonstrate the promise of targeted SWNT nanoprobes for noninvasive disease monitoring and guided surgery. National Institutes of Health (U.S.). Center for Nanotechnology Excellence (Grant U54-CA119349-04) National Institutes of Health (U.S.). Center for Nanotechnology Excellence (Grant U54-CA151884) David H. Koch Institute for Integrative Cancer Research at MIT. Frontier Research Program (Kathy and Curt Marble Cancer Research Fund) National Institute of Environmental Health Sciences (Grant P30-ES002109) Marie D. & Pierre Casimir-Lambert Fund Amar G. Bose Research Grant 2015-04-01T16:53:13Z 2015-04-01T16:53:13Z 2014-09 2014-01 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/96311 Ghosh, D., A. F. Bagley, Y. J. Na, M. J. Birrer, S. N. Bhatia, and A. M. Belcher. “Deep, Noninvasive Imaging and Surgical Guidance of Submillimeter Tumors Using Targeted M13-Stabilized Single-Walled Carbon Nanotubes.” Proceedings of the National Academy of Sciences 111, no. 38 (September 11, 2014): 13948–13953. https://orcid.org/0000-0001-9353-7453 https://orcid.org/0000-0002-0930-302X https://orcid.org/0000-0002-1293-2097 en_US http://dx.doi.org/10.1073/pnas.1400821111 Proceedings of the National Academy of Sciences of the United States of America Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) National Academy of Sciences (U.S.) |
| spellingShingle | Ghosh, Debadyuti Na, Young Jeong Birrer, Michael J. Bagley, Alexander F Bhatia, Sangeeta N Belcher, Angela M Deep, noninvasive imaging and surgical guidance of submillimeter tumors using targeted M13-stabilized single-walled carbon nanotubes |
| title | Deep, noninvasive imaging and surgical guidance of submillimeter tumors using targeted M13-stabilized single-walled carbon nanotubes |
| title_full | Deep, noninvasive imaging and surgical guidance of submillimeter tumors using targeted M13-stabilized single-walled carbon nanotubes |
| title_fullStr | Deep, noninvasive imaging and surgical guidance of submillimeter tumors using targeted M13-stabilized single-walled carbon nanotubes |
| title_full_unstemmed | Deep, noninvasive imaging and surgical guidance of submillimeter tumors using targeted M13-stabilized single-walled carbon nanotubes |
| title_short | Deep, noninvasive imaging and surgical guidance of submillimeter tumors using targeted M13-stabilized single-walled carbon nanotubes |
| title_sort | deep noninvasive imaging and surgical guidance of submillimeter tumors using targeted m13 stabilized single walled carbon nanotubes |
| url | http://hdl.handle.net/1721.1/96311 https://orcid.org/0000-0001-9353-7453 https://orcid.org/0000-0002-0930-302X https://orcid.org/0000-0002-1293-2097 |
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