Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea
Planarians can regenerate any missing body part in a process requiring dividing cells called neoblasts. Historically, neoblasts have largely been considered a homogeneous stem cell population. Most studies, however, analyzed neoblasts at the population rather than the single-cell level, leaving the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2015
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| Online Access: | http://hdl.handle.net/1721.1/96336 https://orcid.org/0000-0002-5569-333X https://orcid.org/0000-0001-9884-4663 |
| _version_ | 1811087339029528576 |
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| author | Scimone, M. Lucila Lapan, Sylvain W. Kravarik, Kellie Marie Reddien, Peter Scimone, M. Lucila Lapan, Sylvain W. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Scimone, M. Lucila Lapan, Sylvain W. Kravarik, Kellie Marie Reddien, Peter Scimone, M. Lucila Lapan, Sylvain W. |
| author_sort | Scimone, M. Lucila |
| collection | MIT |
| description | Planarians can regenerate any missing body part in a process requiring dividing cells called neoblasts. Historically, neoblasts have largely been considered a homogeneous stem cell population. Most studies, however, analyzed neoblasts at the population rather than the single-cell level, leaving the degree of heterogeneity in this population unresolved. We combined RNA sequencing of neoblasts from wounded planarians with expression screening and identified 33 transcription factors transcribed in specific differentiated cells and in small fractions of neoblasts during regeneration. Many neoblast subsets expressing distinct tissue-associated transcription factors were present, suggesting candidate specification into many lineages. Consistent with this possibility, klf, pax3/7, and FoxA were required for the differentiation of cintillo-expressing sensory neurons, dopamine-β-hydroxylase-expressing neurons, and the pharynx, respectively. Together, these results suggest that specification of cell fate for most-to-all regenerative lineages occurs within neoblasts, with regenerative cells of blastemas being generated from a highly heterogeneous collection of lineage-specified neoblasts. |
| first_indexed | 2024-09-23T13:44:30Z |
| format | Article |
| id | mit-1721.1/96336 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:44:30Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/963362022-09-28T15:49:25Z Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea Scimone, M. Lucila Lapan, Sylvain W. Kravarik, Kellie Marie Reddien, Peter Scimone, M. Lucila Lapan, Sylvain W. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Kravarik, Kellie Marie Reddien, Peter Scimone, M. Lucila Lapan, Sylvain W. Planarians can regenerate any missing body part in a process requiring dividing cells called neoblasts. Historically, neoblasts have largely been considered a homogeneous stem cell population. Most studies, however, analyzed neoblasts at the population rather than the single-cell level, leaving the degree of heterogeneity in this population unresolved. We combined RNA sequencing of neoblasts from wounded planarians with expression screening and identified 33 transcription factors transcribed in specific differentiated cells and in small fractions of neoblasts during regeneration. Many neoblast subsets expressing distinct tissue-associated transcription factors were present, suggesting candidate specification into many lineages. Consistent with this possibility, klf, pax3/7, and FoxA were required for the differentiation of cintillo-expressing sensory neurons, dopamine-β-hydroxylase-expressing neurons, and the pharynx, respectively. Together, these results suggest that specification of cell fate for most-to-all regenerative lineages occurs within neoblasts, with regenerative cells of blastemas being generated from a highly heterogeneous collection of lineage-specified neoblasts. National Institutes of Health (U.S.) (R01GM080639) National Science Foundation (U.S.). Graduate Research Fellowship Program (Grant 1122374) 2015-04-02T16:58:56Z 2015-04-02T16:58:56Z 2014-07 2014-06 Article http://purl.org/eprint/type/JournalArticle 22136711 http://hdl.handle.net/1721.1/96336 Scimone, M. Lucila, Kellie M. Kravarik, Sylvain W. Lapan, and Peter W. Reddien. “Neoblast Specialization in Regeneration of the Planarian Schmidtea Mediterranea.” Stem Cell Reports 3, no. 2 (August 2014): 339–352. https://orcid.org/0000-0002-5569-333X https://orcid.org/0000-0001-9884-4663 en_US http://dx.doi.org/10.1016/j.stemcr.2014.06.001 Stem Cell Reports Creative Commons Attribution-Noncommercial-No deriviatives http://creativecommons.org/licenses/by-nc-nd/3.0/ application/pdf Elsevier Elsevier |
| spellingShingle | Scimone, M. Lucila Lapan, Sylvain W. Kravarik, Kellie Marie Reddien, Peter Scimone, M. Lucila Lapan, Sylvain W. Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea |
| title | Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea |
| title_full | Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea |
| title_fullStr | Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea |
| title_full_unstemmed | Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea |
| title_short | Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea |
| title_sort | neoblast specialization in regeneration of the planarian schmidtea mediterranea |
| url | http://hdl.handle.net/1721.1/96336 https://orcid.org/0000-0002-5569-333X https://orcid.org/0000-0001-9884-4663 |
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