The Postsynaptic Density Proteins Homer and Shank Form a Polymeric Network Structure
The postsynaptic density (PSD) is crucial for synaptic functions, but the molecular architecture retaining its structure and components remains elusive. Homer and Shank are among the most abundant scaffolding proteins in the PSD, working synergistically for maturation of dendritic spines. Here, we d...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2015
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| Online Access: | http://hdl.handle.net/1721.1/96340 |
| _version_ | 1811094459467694080 |
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| author | Hayashi, Mariko Kato Tang, Chunyan Verpelli, Chiara Narayanan, Radhakrishnan Stearns, Marissa H. Xu, Rui-Ming Li, Huilin Sala, Carlo Hayashi, Yasunori |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Hayashi, Mariko Kato Tang, Chunyan Verpelli, Chiara Narayanan, Radhakrishnan Stearns, Marissa H. Xu, Rui-Ming Li, Huilin Sala, Carlo Hayashi, Yasunori |
| author_sort | Hayashi, Mariko Kato |
| collection | MIT |
| description | The postsynaptic density (PSD) is crucial for synaptic functions, but the molecular architecture retaining its structure and components remains elusive. Homer and Shank are among the most abundant scaffolding proteins in the PSD, working synergistically for maturation of dendritic spines. Here, we demonstrate that Homer and Shank, together, form a mesh-like matrix structure. Crystallographic analysis of this region revealed a pair of parallel dimeric coiled coils intercalated in a tail-to-tail fashion to form a tetramer, giving rise to the unique configuration of a pair of N-terminal EVH1 domains at each end of the coiled coil. In neurons, the tetramerization is required for structural integrity of the dendritic spines and recruitment of proteins to synapses. We propose that the Homer-Shank complex serves as a structural framework and as an assembly platform for other PSD proteins. |
| first_indexed | 2024-09-23T16:00:25Z |
| format | Article |
| id | mit-1721.1/96340 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:00:25Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/963402022-10-02T05:40:42Z The Postsynaptic Density Proteins Homer and Shank Form a Polymeric Network Structure Hayashi, Mariko Kato Tang, Chunyan Verpelli, Chiara Narayanan, Radhakrishnan Stearns, Marissa H. Xu, Rui-Ming Li, Huilin Sala, Carlo Hayashi, Yasunori Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Picower Institute for Learning and Memory RIKEN-MIT Center for Neural Circuit Genetics Hayashi, Mariko Kato Hayashi, Yasunori Stearns, Marissa H. Narayanan, Radhakrishnan The postsynaptic density (PSD) is crucial for synaptic functions, but the molecular architecture retaining its structure and components remains elusive. Homer and Shank are among the most abundant scaffolding proteins in the PSD, working synergistically for maturation of dendritic spines. Here, we demonstrate that Homer and Shank, together, form a mesh-like matrix structure. Crystallographic analysis of this region revealed a pair of parallel dimeric coiled coils intercalated in a tail-to-tail fashion to form a tetramer, giving rise to the unique configuration of a pair of N-terminal EVH1 domains at each end of the coiled coil. In neurons, the tetramerization is required for structural integrity of the dendritic spines and recruitment of proteins to synapses. We propose that the Homer-Shank complex serves as a structural framework and as an assembly platform for other PSD proteins. Massachusetts Institute of Technology. Biophysical Instrumentation Facility RIKEN Brain Science Institute National Institutes of Health (U.S.) (Grant R01DA17310) Japan. Ministry of Education, Culture, Sports, Science and Technology (Grant-in-Aid for Scientific Research) 2015-04-02T17:28:12Z 2015-04-02T17:28:12Z 2009-04 2008-11 Article http://purl.org/eprint/type/JournalArticle 00928674 1097-4172 http://hdl.handle.net/1721.1/96340 Hayashi, Mariko Kato, Chunyan Tang, Chiara Verpelli, Radhakrishnan Narayanan, Marissa H. Stearns, Rui-Ming Xu, Huilin Li, Carlo Sala, and Yasunori Hayashi. “The Postsynaptic Density Proteins Homer and Shank Form a Polymeric Network Structure.” Cell 137, no. 1 (April 2009): 159–171. © 2009 Elsevier Inc. en_US http://dx.doi.org/10.1016/j.cell.2009.01.050 Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier |
| spellingShingle | Hayashi, Mariko Kato Tang, Chunyan Verpelli, Chiara Narayanan, Radhakrishnan Stearns, Marissa H. Xu, Rui-Ming Li, Huilin Sala, Carlo Hayashi, Yasunori The Postsynaptic Density Proteins Homer and Shank Form a Polymeric Network Structure |
| title | The Postsynaptic Density Proteins Homer and Shank Form a Polymeric Network Structure |
| title_full | The Postsynaptic Density Proteins Homer and Shank Form a Polymeric Network Structure |
| title_fullStr | The Postsynaptic Density Proteins Homer and Shank Form a Polymeric Network Structure |
| title_full_unstemmed | The Postsynaptic Density Proteins Homer and Shank Form a Polymeric Network Structure |
| title_short | The Postsynaptic Density Proteins Homer and Shank Form a Polymeric Network Structure |
| title_sort | postsynaptic density proteins homer and shank form a polymeric network structure |
| url | http://hdl.handle.net/1721.1/96340 |
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