Noninvasive glucose sensing by transcutaneous Raman spectroscopy
We present the development of a transcutaneous Raman spectroscopy system and analysis algorithm for noninvasive glucose sensing. The instrument and algorithm were tested in a preclinical study in which a dog model was used. To achieve a robust glucose test system, the blood levels were clamped for p...
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| Format: | Article |
| Language: | en_US |
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Society of Photo-Optical Instrumentation Engineers (SPIE)
2015
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| Online Access: | http://hdl.handle.net/1721.1/96380 |
| _version_ | 1811087575208689664 |
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| author | Shih, Wei-Chuan Bechtel, Kate L. Rebec, Mihailo V. |
| author2 | Massachusetts Institute of Technology. Spectroscopy Laboratory |
| author_facet | Massachusetts Institute of Technology. Spectroscopy Laboratory Shih, Wei-Chuan Bechtel, Kate L. Rebec, Mihailo V. |
| author_sort | Shih, Wei-Chuan |
| collection | MIT |
| description | We present the development of a transcutaneous Raman spectroscopy system and analysis algorithm for noninvasive glucose sensing. The instrument and algorithm were tested in a preclinical study in which a dog model was used. To achieve a robust glucose test system, the blood levels were clamped for periods of up to 45 min. Glucose clamping and rise/fall patterns have been achieved by injecting glucose and insulin into the ear veins of the dog. Venous blood samples were drawn every 5 min and a plasma glucose concentration was obtained and used to maintain the clamps, to build the calibration model, and to evaluate the performance of the system. We evaluated the utility of the simultaneously acquired Raman spectra to be used to determine the plasma glucose values during the 8-h experiment. We obtained prediction errors in the range of ∼1.5−2 mM. These were in-line with a best-case theoretical estimate considering the limitations of the signal-to-noise ratio estimates. As expected, the transition regions of the clamp study produced larger predictive errors than the stable regions. This is related to the divergence of the interstitial fluid (ISF) and plasma glucose values during those periods. Two key contributors to error beside the ISF/plasma difference were photobleaching and detector drift. The study demonstrated the potential of Raman spectroscopy in noninvasive applications and provides areas where the technology can be improved in future studies. |
| first_indexed | 2024-09-23T13:48:16Z |
| format | Article |
| id | mit-1721.1/96380 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:48:16Z |
| publishDate | 2015 |
| publisher | Society of Photo-Optical Instrumentation Engineers (SPIE) |
| record_format | dspace |
| spelling | mit-1721.1/963802022-09-28T16:17:52Z Noninvasive glucose sensing by transcutaneous Raman spectroscopy Shih, Wei-Chuan Bechtel, Kate L. Rebec, Mihailo V. Massachusetts Institute of Technology. Spectroscopy Laboratory Shih, Wei-Chuan Bechtel, Kate L. We present the development of a transcutaneous Raman spectroscopy system and analysis algorithm for noninvasive glucose sensing. The instrument and algorithm were tested in a preclinical study in which a dog model was used. To achieve a robust glucose test system, the blood levels were clamped for periods of up to 45 min. Glucose clamping and rise/fall patterns have been achieved by injecting glucose and insulin into the ear veins of the dog. Venous blood samples were drawn every 5 min and a plasma glucose concentration was obtained and used to maintain the clamps, to build the calibration model, and to evaluate the performance of the system. We evaluated the utility of the simultaneously acquired Raman spectra to be used to determine the plasma glucose values during the 8-h experiment. We obtained prediction errors in the range of ∼1.5−2 mM. These were in-line with a best-case theoretical estimate considering the limitations of the signal-to-noise ratio estimates. As expected, the transition regions of the clamp study produced larger predictive errors than the stable regions. This is related to the divergence of the interstitial fluid (ISF) and plasma glucose values during those periods. Two key contributors to error beside the ISF/plasma difference were photobleaching and detector drift. The study demonstrated the potential of Raman spectroscopy in noninvasive applications and provides areas where the technology can be improved in future studies. National Science Foundation (U.S.) (CAREER Award (No. CBET-1151154)) United States. National Aeronautics and Space Administration (Early Career Faculty Grant (No. NNX12AQ44G)) Gulf of Mexico Research Initiative (GoMRI-030) Cullen College of Engineering 2015-04-03T18:29:13Z 2015-04-03T18:29:13Z 2015-02 2014-10 Article http://purl.org/eprint/type/JournalArticle 1083-3668 http://hdl.handle.net/1721.1/96380 Shih, Wei-Chuan, Kate L. Bechtel, and Mihailo V. Rebec. “Noninvasive Glucose Sensing by Transcutaneous Raman Spectroscopy.” Journal of Biomedical Optics 20, no. 5 (February 17, 2015): 051036. © 2015 SPIE. en_US http://dx.doi.org/10.1117/1.jbo.20.5.051036 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Society of Photo-Optical Instrumentation Engineers (SPIE) SPIE |
| spellingShingle | Shih, Wei-Chuan Bechtel, Kate L. Rebec, Mihailo V. Noninvasive glucose sensing by transcutaneous Raman spectroscopy |
| title | Noninvasive glucose sensing by transcutaneous Raman spectroscopy |
| title_full | Noninvasive glucose sensing by transcutaneous Raman spectroscopy |
| title_fullStr | Noninvasive glucose sensing by transcutaneous Raman spectroscopy |
| title_full_unstemmed | Noninvasive glucose sensing by transcutaneous Raman spectroscopy |
| title_short | Noninvasive glucose sensing by transcutaneous Raman spectroscopy |
| title_sort | noninvasive glucose sensing by transcutaneous raman spectroscopy |
| url | http://hdl.handle.net/1721.1/96380 |
| work_keys_str_mv | AT shihweichuan noninvasiveglucosesensingbytranscutaneousramanspectroscopy AT bechtelkatel noninvasiveglucosesensingbytranscutaneousramanspectroscopy AT rebecmihailov noninvasiveglucosesensingbytranscutaneousramanspectroscopy |