Transcriptional profiling of cells sorted by transcript abundance
We have developed a quantitative technique for sorting cells on the basis of endogenous RNA abundance, with a molecular resolution of 10–20 transcripts. We demonstrate efficient and unbiased RNA extraction from transcriptionally sorted cells and report a high-fidelity transcriptome measurement of mo...
Main Authors: | Klemm, Sandy Lee, Semrau, Stefan, Wiebrands, Kay, Mooijman, Dylan, Jaenisch, Rudolf, van Oudenaarden, Alexander, Faddah, Dina A. |
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Other Authors: | Massachusetts Institute of Technology. Department of Biology |
Format: | Article |
Language: | en_US |
Published: |
Nature Publishing Group
2015
|
Online Access: | http://hdl.handle.net/1721.1/96519 |
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