Striatins as plaque molecules of zonulae adhaerentes in simple epithelia, of tessellate junctions in stratified epithelia, of cardiac composite junctions and of various size classes of lateral adherens junctions in cultures of epithelia- and carcinoma-derived cells
Proteins of the striatin family (striatins 1–4; sizes ranging from 90 to 110 kDa on SDS-polyacrylamide gel electrophoresis) are highly homologous in their amino acid sequences but can differ in their cell-type-specific gene expression patterns and biological functions. In various cell types, we have...
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| Format: | Article |
| Language: | en_US |
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Springer-Verlag Berlin Heidelberg
2015
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| Online Access: | http://hdl.handle.net/1721.1/96528 https://orcid.org/0000-0002-5224-7764 |
| _version_ | 1826208773951193088 |
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| author | Franke, Werner W. Rickelt, Steffen Zimbelmann, Ralf Dörflinger, Yvette Kuhn, Caecilia Frey, Norbert Heid, Hans Rosin-Arbesfeld, Rina |
| author2 | Koch Institute for Integrative Cancer Research at MIT |
| author_facet | Koch Institute for Integrative Cancer Research at MIT Franke, Werner W. Rickelt, Steffen Zimbelmann, Ralf Dörflinger, Yvette Kuhn, Caecilia Frey, Norbert Heid, Hans Rosin-Arbesfeld, Rina |
| author_sort | Franke, Werner W. |
| collection | MIT |
| description | Proteins of the striatin family (striatins 1–4; sizes ranging from 90 to 110 kDa on SDS-polyacrylamide gel electrophoresis) are highly homologous in their amino acid sequences but can differ in their cell-type-specific gene expression patterns and biological functions. In various cell types, we have found one, two or three polypeptides of this evolutionarily old and nearly ubiquitous family of proteins known to serve as scaffold proteins for diverse protein complexes. Light and electron microscopic immunolocalization methods have revealed striatins in mammalian cell-cell adherens junctions (AJs). In simple epithelia, we have localized striatins as constitutive components of the plaques of the subapical zonulae adhaerentes of cells, including intestinal, glandular, ductal and urothelial cells and hepatocytes. Striatins colocalize with E-cadherin or E–N-cadherin heterodimers and with the plaque proteins α- and β-catenin, p120 and p0071. In some epithelia and carcinomas and in cultured cells derived therefrom, striatins are also seen in lateral AJs. In stratified epithelia and in corresponding squamous cell carcinomas, striatins can be found in plaques of some forms of tessellate junctions. Moreover, striatins are major plaque proteins of composite junctions (CJs; areae compositae) in the intercalated disks connecting cardiomyocytes, colocalizing with other CJ molecules, including plectin and ankyrin-G. We discuss the “multimodulator” scaffold roles of striatins in the initiation and regulation of the formation of various complex particles and structures. We propose that striatins are included in the diagnostic candidate list of proteins that, in the CJs of human hearts, can occur in mutated forms in the pathogeneses of hereditary cardiomyopathies, as seen in some types of genetically determined heart damage in boxer dogs. |
| first_indexed | 2024-09-23T14:12:26Z |
| format | Article |
| id | mit-1721.1/96528 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:12:26Z |
| publishDate | 2015 |
| publisher | Springer-Verlag Berlin Heidelberg |
| record_format | dspace |
| spelling | mit-1721.1/965282022-09-28T19:09:29Z Striatins as plaque molecules of zonulae adhaerentes in simple epithelia, of tessellate junctions in stratified epithelia, of cardiac composite junctions and of various size classes of lateral adherens junctions in cultures of epithelia- and carcinoma-derived cells Franke, Werner W. Rickelt, Steffen Zimbelmann, Ralf Dörflinger, Yvette Kuhn, Caecilia Frey, Norbert Heid, Hans Rosin-Arbesfeld, Rina Koch Institute for Integrative Cancer Research at MIT Rickelt, Steffen Proteins of the striatin family (striatins 1–4; sizes ranging from 90 to 110 kDa on SDS-polyacrylamide gel electrophoresis) are highly homologous in their amino acid sequences but can differ in their cell-type-specific gene expression patterns and biological functions. In various cell types, we have found one, two or three polypeptides of this evolutionarily old and nearly ubiquitous family of proteins known to serve as scaffold proteins for diverse protein complexes. Light and electron microscopic immunolocalization methods have revealed striatins in mammalian cell-cell adherens junctions (AJs). In simple epithelia, we have localized striatins as constitutive components of the plaques of the subapical zonulae adhaerentes of cells, including intestinal, glandular, ductal and urothelial cells and hepatocytes. Striatins colocalize with E-cadherin or E–N-cadherin heterodimers and with the plaque proteins α- and β-catenin, p120 and p0071. In some epithelia and carcinomas and in cultured cells derived therefrom, striatins are also seen in lateral AJs. In stratified epithelia and in corresponding squamous cell carcinomas, striatins can be found in plaques of some forms of tessellate junctions. Moreover, striatins are major plaque proteins of composite junctions (CJs; areae compositae) in the intercalated disks connecting cardiomyocytes, colocalizing with other CJ molecules, including plectin and ankyrin-G. We discuss the “multimodulator” scaffold roles of striatins in the initiation and regulation of the formation of various complex particles and structures. We propose that striatins are included in the diagnostic candidate list of proteins that, in the CJs of human hearts, can occur in mutated forms in the pathogeneses of hereditary cardiomyopathies, as seen in some types of genetically determined heart damage in boxer dogs. German-Israeli Foundation for Scientific Research and Development (GIF grant I-1098-43.11/2010) 2015-04-13T14:31:10Z 2015-04-13T14:31:10Z 2014-12 2014-07 Article http://purl.org/eprint/type/JournalArticle 0302-766X 1432-0878 http://hdl.handle.net/1721.1/96528 Franke, Werner W., Steffen Rickelt, Ralf Zimbelmann, Yvette Dörflinger, Caecilia Kuhn, Norbert Frey, Hans Heid, and Rina Rosin-Arbesfeld. “Striatins as Plaque Molecules of Zonulae Adhaerentes in Simple Epithelia, of Tessellate Junctions in Stratified Epithelia, of Cardiac Composite Junctions and of Various Size Classes of Lateral Adherens Junctions in Cultures of Epithelia- and Carcinoma-Derived Cells.” Cell and Tissue Research 359, no. 3 (December 12, 2014): 779–797. https://orcid.org/0000-0002-5224-7764 en_US http://dx.doi.org/10.1007/s00441-014-2053-z Cell and Tissue Research Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Springer-Verlag Berlin Heidelberg Springer-Verlag |
| spellingShingle | Franke, Werner W. Rickelt, Steffen Zimbelmann, Ralf Dörflinger, Yvette Kuhn, Caecilia Frey, Norbert Heid, Hans Rosin-Arbesfeld, Rina Striatins as plaque molecules of zonulae adhaerentes in simple epithelia, of tessellate junctions in stratified epithelia, of cardiac composite junctions and of various size classes of lateral adherens junctions in cultures of epithelia- and carcinoma-derived cells |
| title | Striatins as plaque molecules of zonulae adhaerentes in simple epithelia, of tessellate junctions in stratified epithelia, of cardiac composite junctions and of various size classes of lateral adherens junctions in cultures of epithelia- and carcinoma-derived cells |
| title_full | Striatins as plaque molecules of zonulae adhaerentes in simple epithelia, of tessellate junctions in stratified epithelia, of cardiac composite junctions and of various size classes of lateral adherens junctions in cultures of epithelia- and carcinoma-derived cells |
| title_fullStr | Striatins as plaque molecules of zonulae adhaerentes in simple epithelia, of tessellate junctions in stratified epithelia, of cardiac composite junctions and of various size classes of lateral adherens junctions in cultures of epithelia- and carcinoma-derived cells |
| title_full_unstemmed | Striatins as plaque molecules of zonulae adhaerentes in simple epithelia, of tessellate junctions in stratified epithelia, of cardiac composite junctions and of various size classes of lateral adherens junctions in cultures of epithelia- and carcinoma-derived cells |
| title_short | Striatins as plaque molecules of zonulae adhaerentes in simple epithelia, of tessellate junctions in stratified epithelia, of cardiac composite junctions and of various size classes of lateral adherens junctions in cultures of epithelia- and carcinoma-derived cells |
| title_sort | striatins as plaque molecules of zonulae adhaerentes in simple epithelia of tessellate junctions in stratified epithelia of cardiac composite junctions and of various size classes of lateral adherens junctions in cultures of epithelia and carcinoma derived cells |
| url | http://hdl.handle.net/1721.1/96528 https://orcid.org/0000-0002-5224-7764 |
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