Molecular Adhesion between Cartilage Extracellular Matrix Macromolecules
In this study, we investigated the molecular adhesion between the major constituents of cartilage extracellular matrix, namely, the highly negatively charged proteoglycan aggrecan and the type II/IX/XI fibrillar collagen network, in simulated physiological conditions. Colloidal force spectroscopy wa...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2015
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| Online Access: | http://hdl.handle.net/1721.1/96694 https://orcid.org/0000-0003-3511-5679 https://orcid.org/0000-0002-4942-3456 |
| _version_ | 1826204602859520000 |
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| author | Rojas, Fredrick P. Batista, Michael A. Lindburg, C. Alexander Dean, Delphine Grodzinsky, Alan J. Ortiz, Christine Han, Lin |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Rojas, Fredrick P. Batista, Michael A. Lindburg, C. Alexander Dean, Delphine Grodzinsky, Alan J. Ortiz, Christine Han, Lin |
| author_sort | Rojas, Fredrick P. |
| collection | MIT |
| description | In this study, we investigated the molecular adhesion between the major constituents of cartilage extracellular matrix, namely, the highly negatively charged proteoglycan aggrecan and the type II/IX/XI fibrillar collagen network, in simulated physiological conditions. Colloidal force spectroscopy was applied to measure the maximum adhesion force and total adhesion energy between aggrecan end-attached spherical tips (end radius R ≈ 2.5 μm) and trypsin-treated cartilage disks with undamaged collagen networks. Studies were carried out in various aqueous solutions to reveal the physical factors that govern aggrecan–collagen adhesion. Increasing both ionic strength and [Ca2+] significantly increased adhesion, highlighting the importance of electrostatic repulsion and Ca2+-mediated ion bridging effects. In addition, we probed how partial enzymatic degradation of the collagen network, which simulates osteoarthritic conditions, affects the aggrecan–collagen interactions. Interestingly, we found a significant increase in aggrecan–collagen adhesion even when there were no detectable changes at the macro- or microscales. It is hypothesized that the aggrecan–collagen adhesion, together with aggrecan–aggrecan self-adhesion, works synergistically to determine the local molecular deformability and energy dissipation of the cartilage matrix, in turn, affecting its macroscopic tissue properties. |
| first_indexed | 2024-09-23T12:57:45Z |
| format | Article |
| id | mit-1721.1/96694 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:57:45Z |
| publishDate | 2015 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/966942022-10-01T12:11:01Z Molecular Adhesion between Cartilage Extracellular Matrix Macromolecules Rojas, Fredrick P. Batista, Michael A. Lindburg, C. Alexander Dean, Delphine Grodzinsky, Alan J. Ortiz, Christine Han, Lin Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Materials Science and Engineering Rojas, Fredrick P. Batista, Michael A. Grodzinsky, Alan J. Ortiz, Christine Han, Lin In this study, we investigated the molecular adhesion between the major constituents of cartilage extracellular matrix, namely, the highly negatively charged proteoglycan aggrecan and the type II/IX/XI fibrillar collagen network, in simulated physiological conditions. Colloidal force spectroscopy was applied to measure the maximum adhesion force and total adhesion energy between aggrecan end-attached spherical tips (end radius R ≈ 2.5 μm) and trypsin-treated cartilage disks with undamaged collagen networks. Studies were carried out in various aqueous solutions to reveal the physical factors that govern aggrecan–collagen adhesion. Increasing both ionic strength and [Ca2+] significantly increased adhesion, highlighting the importance of electrostatic repulsion and Ca2+-mediated ion bridging effects. In addition, we probed how partial enzymatic degradation of the collagen network, which simulates osteoarthritic conditions, affects the aggrecan–collagen interactions. Interestingly, we found a significant increase in aggrecan–collagen adhesion even when there were no detectable changes at the macro- or microscales. It is hypothesized that the aggrecan–collagen adhesion, together with aggrecan–aggrecan self-adhesion, works synergistically to determine the local molecular deformability and energy dissipation of the cartilage matrix, in turn, affecting its macroscopic tissue properties. National Science Foundation (U.S.) (Grant CMMI-0758651) National Institutes of Health (U.S.) (Grant AR60331) United States. Dept. of Defense (National Defense Science and Engineering Graduate Fellowship (Grant N00244-09-1-0064)) Shriners of North America Drexel University (Faculty Start-up Grant) 2015-04-22T15:33:47Z 2015-04-22T15:33:47Z 2014-03 2014-01 Article http://purl.org/eprint/type/JournalArticle 1525-7797 1526-4602 http://hdl.handle.net/1721.1/96694 Rojas, Fredrick P., Michael A. Batista, C. Alexander Lindburg, Delphine Dean, Alan J. Grodzinsky, Christine Ortiz, and Lin Han. “Molecular Adhesion Between Cartilage Extracellular Matrix Macromolecules.” Biomacromolecules 15, no. 3 (March 10, 2014): 772–780. © 2014 American Chemical Society. https://orcid.org/0000-0003-3511-5679 https://orcid.org/0000-0002-4942-3456 en_US http://dx.doi.org/10.1021/bm401611b Biomacromolecules application/pdf American Chemical Society (ACS) American Chemical Society |
| spellingShingle | Rojas, Fredrick P. Batista, Michael A. Lindburg, C. Alexander Dean, Delphine Grodzinsky, Alan J. Ortiz, Christine Han, Lin Molecular Adhesion between Cartilage Extracellular Matrix Macromolecules |
| title | Molecular Adhesion between Cartilage Extracellular Matrix Macromolecules |
| title_full | Molecular Adhesion between Cartilage Extracellular Matrix Macromolecules |
| title_fullStr | Molecular Adhesion between Cartilage Extracellular Matrix Macromolecules |
| title_full_unstemmed | Molecular Adhesion between Cartilage Extracellular Matrix Macromolecules |
| title_short | Molecular Adhesion between Cartilage Extracellular Matrix Macromolecules |
| title_sort | molecular adhesion between cartilage extracellular matrix macromolecules |
| url | http://hdl.handle.net/1721.1/96694 https://orcid.org/0000-0003-3511-5679 https://orcid.org/0000-0002-4942-3456 |
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