Fully-automated in vivo single cell electrophysiology
In this work, we report progress in developing a device that allows fully autonomous sequential patch clamp experimentation. The machine works by integrating a storage magazine of pre-filled pipettes that can be accessed, and swapped, by the headstage at the conclusion of each experiment. In operati...
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Society for Precision Engineering
2015
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| Online Access: | http://hdl.handle.net/1721.1/96716 https://orcid.org/0000-0003-2218-7489 https://orcid.org/0000-0002-0419-3351 |
| Summary: | In this work, we report progress in developing a device that allows fully autonomous sequential patch clamp experimentation. The machine works by integrating a storage magazine of pre-filled pipettes that can be accessed, and swapped, by the headstage at the conclusion of each experiment. In operation, following each neuron measurement, the program enters “swap” state where a set of programmed actuator movements take place. First, the headstage translates towards the pipette storage assembly and deposits its used pipette. The storage assembly rotates to index a fresh pipette, its is grasped, and finally, the headstage returns to its previously designated home position in preparation of subsequent experiments. |
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