Fully-automated in vivo single cell electrophysiology
In this work, we report progress in developing a device that allows fully autonomous sequential patch clamp experimentation. The machine works by integrating a storage magazine of pre-filled pipettes that can be accessed, and swapped, by the headstage at the conclusion of each experiment. In operati...
| Main Authors: | , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Society for Precision Engineering
2015
|
| Online Access: | http://hdl.handle.net/1721.1/96716 https://orcid.org/0000-0003-2218-7489 https://orcid.org/0000-0002-0419-3351 |
| _version_ | 1811091413057667072 |
|---|---|
| author | Go, Jamison Fan, Aaron Lu, Coby Kodandaramaiah, Suhasa Bangalo Holst, Gregory L. Stoy, William Kolb, Ilya Forest, Craig R. Boyden, Edward |
| author2 | Massachusetts Institute of Technology. Media Laboratory |
| author_facet | Massachusetts Institute of Technology. Media Laboratory Go, Jamison Fan, Aaron Lu, Coby Kodandaramaiah, Suhasa Bangalo Holst, Gregory L. Stoy, William Kolb, Ilya Forest, Craig R. Boyden, Edward |
| author_sort | Go, Jamison |
| collection | MIT |
| description | In this work, we report progress in developing a device that allows fully autonomous sequential patch clamp experimentation. The machine works by integrating a storage magazine of pre-filled pipettes that can be accessed, and swapped, by the headstage at the conclusion of each experiment. In operation, following each neuron measurement, the program enters “swap” state where a set of programmed actuator movements take place. First, the headstage translates towards the pipette storage assembly and deposits its used pipette. The storage assembly rotates to index a fresh pipette, its is grasped, and finally, the headstage returns to its previously designated home position in preparation of subsequent experiments. |
| first_indexed | 2024-09-23T15:02:01Z |
| format | Article |
| id | mit-1721.1/96716 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:02:01Z |
| publishDate | 2015 |
| publisher | American Society for Precision Engineering |
| record_format | dspace |
| spelling | mit-1721.1/967162022-10-02T00:05:21Z Fully-automated in vivo single cell electrophysiology Go, Jamison Fan, Aaron Lu, Coby Kodandaramaiah, Suhasa Bangalo Holst, Gregory L. Stoy, William Kolb, Ilya Forest, Craig R. Boyden, Edward Massachusetts Institute of Technology. Media Laboratory McGovern Institute for Brain Research at MIT Program in Media Arts and Sciences (Massachusetts Institute of Technology) Boyden, Edward Stuart Kodandaramaiah, Suhasa Bangalo Boyden, Edward Stuart In this work, we report progress in developing a device that allows fully autonomous sequential patch clamp experimentation. The machine works by integrating a storage magazine of pre-filled pipettes that can be accessed, and swapped, by the headstage at the conclusion of each experiment. In operation, following each neuron measurement, the program enters “swap” state where a set of programmed actuator movements take place. First, the headstage translates towards the pipette storage assembly and deposits its used pipette. The storage assembly rotates to index a fresh pipette, its is grasped, and finally, the headstage returns to its previously designated home position in preparation of subsequent experiments. 2015-04-22T19:53:39Z 2015-04-22T19:53:39Z 2013-10 Article http://purl.org/eprint/type/ConferencePaper http://hdl.handle.net/1721.1/96716 Go, Jamison, Aaron Fan, Coby Lu, Suhasa Kodandaramaiah, Gregory L. Holst, William Stoy, Ilya Kolb, Edward S. Boyden, and Craig R. Forest (2013). "Fully-automated, in-vivo, single cell electrophysiology" in Proceedings of the 28th Annual Meeting of the American Society for Precision Engineering, Saint Paul, MN, Oct 20-25, 2013. https://orcid.org/0000-0003-2218-7489 https://orcid.org/0000-0002-0419-3351 en_US http://www.aspe.net/publications/Short%20Abstracts%2013A/3832.pdf Proceedings of the 28th Annual Meeting of the American Society for Precision Engineering Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Society for Precision Engineering Boyden via Patsy Baudoin |
| spellingShingle | Go, Jamison Fan, Aaron Lu, Coby Kodandaramaiah, Suhasa Bangalo Holst, Gregory L. Stoy, William Kolb, Ilya Forest, Craig R. Boyden, Edward Fully-automated in vivo single cell electrophysiology |
| title | Fully-automated in vivo single cell electrophysiology |
| title_full | Fully-automated in vivo single cell electrophysiology |
| title_fullStr | Fully-automated in vivo single cell electrophysiology |
| title_full_unstemmed | Fully-automated in vivo single cell electrophysiology |
| title_short | Fully-automated in vivo single cell electrophysiology |
| title_sort | fully automated in vivo single cell electrophysiology |
| url | http://hdl.handle.net/1721.1/96716 https://orcid.org/0000-0003-2218-7489 https://orcid.org/0000-0002-0419-3351 |
| work_keys_str_mv | AT gojamison fullyautomatedinvivosinglecellelectrophysiology AT fanaaron fullyautomatedinvivosinglecellelectrophysiology AT lucoby fullyautomatedinvivosinglecellelectrophysiology AT kodandaramaiahsuhasabangalo fullyautomatedinvivosinglecellelectrophysiology AT holstgregoryl fullyautomatedinvivosinglecellelectrophysiology AT stoywilliam fullyautomatedinvivosinglecellelectrophysiology AT kolbilya fullyautomatedinvivosinglecellelectrophysiology AT forestcraigr fullyautomatedinvivosinglecellelectrophysiology AT boydenedward fullyautomatedinvivosinglecellelectrophysiology |