Continuous release of gentamicin from gold nanocarriers
Antibiotics are still the most effective agents used to fight bacterial infections. Antibiotics are quickly metabolised or excreted from the human body, thus they need to be frequently administered (a few times a day) and their half life is usually an important factor in the therapeutic choice. In o...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Royal Society of Chemistry
2015
|
| Online Access: | http://hdl.handle.net/1721.1/96747 |
| _version_ | 1811077418080796672 |
|---|---|
| author | Perni, Stefano Prokopovich, Polina |
| author2 | Massachusetts Institute of Technology. Center for Biomedical Engineering |
| author_facet | Massachusetts Institute of Technology. Center for Biomedical Engineering Perni, Stefano Prokopovich, Polina |
| author_sort | Perni, Stefano |
| collection | MIT |
| description | Antibiotics are still the most effective agents used to fight bacterial infections. Antibiotics are quickly metabolised or excreted from the human body, thus they need to be frequently administered (a few times a day) and their half life is usually an important factor in the therapeutic choice. In order to render the administration less frequent, antibiotic release from a carrier can be employed. In this work we covalently bound gentamicin to gold nanoparticles capped with cysteine or glutathione as gold nanoparticles are biologically safe. The conjugates exhibited antimicrobial activity against both S. aureus and MRSA at concentrations as low as 0.1 mg NP per ml consistent with an antibiotic load of 1–2% w/w as determined through TGA. No antimicrobial activity was exhibited by the unconjugated nanoparticles. The release of gentamicin from the conjugates was monitor in buffer solutions at pH = 7 and the antibiotic concentration continued to increase over two days. This work demonstrates that gold nanoparticles can be employed as antibiotic carriers providing a continuous release of antibiotic over a few days. Glutathione appeared to be a better coupling agent than cysteine allowing a higher load of gentamicin resulting in lower inhibitory concentrations of the conjugates. |
| first_indexed | 2024-09-23T10:42:42Z |
| format | Article |
| id | mit-1721.1/96747 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:42:42Z |
| publishDate | 2015 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/967472022-09-27T14:24:49Z Continuous release of gentamicin from gold nanocarriers Perni, Stefano Prokopovich, Polina Massachusetts Institute of Technology. Center for Biomedical Engineering Perni, Stefano Prokopovich, Polina Antibiotics are still the most effective agents used to fight bacterial infections. Antibiotics are quickly metabolised or excreted from the human body, thus they need to be frequently administered (a few times a day) and their half life is usually an important factor in the therapeutic choice. In order to render the administration less frequent, antibiotic release from a carrier can be employed. In this work we covalently bound gentamicin to gold nanoparticles capped with cysteine or glutathione as gold nanoparticles are biologically safe. The conjugates exhibited antimicrobial activity against both S. aureus and MRSA at concentrations as low as 0.1 mg NP per ml consistent with an antibiotic load of 1–2% w/w as determined through TGA. No antimicrobial activity was exhibited by the unconjugated nanoparticles. The release of gentamicin from the conjugates was monitor in buffer solutions at pH = 7 and the antibiotic concentration continued to increase over two days. This work demonstrates that gold nanoparticles can be employed as antibiotic carriers providing a continuous release of antibiotic over a few days. Glutathione appeared to be a better coupling agent than cysteine allowing a higher load of gentamicin resulting in lower inhibitory concentrations of the conjugates. Arthritis Research UK (ARUK::18461) 2015-04-23T17:46:19Z 2015-04-23T17:46:19Z 2014-10 2014-09 Article http://purl.org/eprint/type/JournalArticle 2046-2069 http://hdl.handle.net/1721.1/96747 Perni, Stefano, and Polina Prokopovich. “Continuous Release of Gentamicin from Gold Nanocarriers.” RSC Advances 4, no. 94 (2014): 51904–51910. en_US http://dx.doi.org/10.1039/c4ra10023a RSC Advances Creative Commons Attribution http://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry Royal Society of Chemistry |
| spellingShingle | Perni, Stefano Prokopovich, Polina Continuous release of gentamicin from gold nanocarriers |
| title | Continuous release of gentamicin from gold nanocarriers |
| title_full | Continuous release of gentamicin from gold nanocarriers |
| title_fullStr | Continuous release of gentamicin from gold nanocarriers |
| title_full_unstemmed | Continuous release of gentamicin from gold nanocarriers |
| title_short | Continuous release of gentamicin from gold nanocarriers |
| title_sort | continuous release of gentamicin from gold nanocarriers |
| url | http://hdl.handle.net/1721.1/96747 |
| work_keys_str_mv | AT pernistefano continuousreleaseofgentamicinfromgoldnanocarriers AT prokopovichpolina continuousreleaseofgentamicinfromgoldnanocarriers |