Optimized E.coli expression strain LOBSTR eliminates common contaminants from His-tag purification
His-tag affinity purification is one of the most commonly used methods to purify recombinant proteins expressed in E. coli. One drawback of using the His-tag is the co-purification of contaminating histidine-rich E. coli proteins. We engineered a new E. coli expression strain, LOBSTR (low background...
Main Authors: | Andersen, Kasper R., Leksa, Nina Carolina, Schwartz, Thomas |
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Other Authors: | Massachusetts Institute of Technology. Department of Biology |
Format: | Article |
Language: | en_US |
Published: |
Wiley Blackwell
2015
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Online Access: | http://hdl.handle.net/1721.1/96797 https://orcid.org/0000-0001-8012-1512 |
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