Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels
Triacylglycerols (TAGs) are in the spotlight as a feasible source of hydrocarbon-based biofuels. Rhodococcus opacus PD630 produces large amounts of intracellular TAGs in cultivations containing high concentrations of glucose, but it does not utilize xylose present in all hydrolysates of lignocellulo...
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| Format: | Article |
| Language: | en_US |
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OMICS Publishing Group
2015
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| Online Access: | http://hdl.handle.net/1721.1/96819 https://orcid.org/0000-0002-1015-1270 |
| _version_ | 1811088762649706496 |
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| author | Kurosawa, Kazuhiko Wewetzer, Sandra J. Sinskey, Anthony J |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Kurosawa, Kazuhiko Wewetzer, Sandra J. Sinskey, Anthony J |
| author_sort | Kurosawa, Kazuhiko |
| collection | MIT |
| description | Triacylglycerols (TAGs) are in the spotlight as a feasible source of hydrocarbon-based biofuels. Rhodococcus opacus PD630 produces large amounts of intracellular TAGs in cultivations containing high concentrations of glucose, but it does not utilize xylose present in all hydrolysates of lignocellulosic biomass. We constructed a highpotency xylose-fermenting R. opacus strain MITXM-61 that exhibited robust growth and TAG biosynthesis on high concentrations of xylose by activating potential xylose-metabolism genes. MITXM-61 had the uncommon capacity to grow in defined media supplemented with xylose at concentrations of greater than 200 gl-1. MITXM-61 grown in corn stover hydrolysates containing 118 gl-1 of initial total sugars was capable of completely and simultaneously utilizing both xylose and glucose in the genuine lignocellulosic feedstock, and yielded 15.9 gl-1 of TAGs, corresponding to 54% of the cell dry weight. The oleaginous bacterium R. opacus strain proved useful for developing a new manufacturing paradigm to generate advanced lignocellulosic biofuels. |
| first_indexed | 2024-09-23T14:07:09Z |
| format | Article |
| id | mit-1721.1/96819 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:07:09Z |
| publishDate | 2015 |
| publisher | OMICS Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/968192022-09-28T18:36:45Z Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels Kurosawa, Kazuhiko Wewetzer, Sandra J. Sinskey, Anthony J Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Engineering Systems Division Kurosawa, Kazuhiko Sinskey, Anthony J. Triacylglycerols (TAGs) are in the spotlight as a feasible source of hydrocarbon-based biofuels. Rhodococcus opacus PD630 produces large amounts of intracellular TAGs in cultivations containing high concentrations of glucose, but it does not utilize xylose present in all hydrolysates of lignocellulosic biomass. We constructed a highpotency xylose-fermenting R. opacus strain MITXM-61 that exhibited robust growth and TAG biosynthesis on high concentrations of xylose by activating potential xylose-metabolism genes. MITXM-61 had the uncommon capacity to grow in defined media supplemented with xylose at concentrations of greater than 200 gl-1. MITXM-61 grown in corn stover hydrolysates containing 118 gl-1 of initial total sugars was capable of completely and simultaneously utilizing both xylose and glucose in the genuine lignocellulosic feedstock, and yielded 15.9 gl-1 of TAGs, corresponding to 54% of the cell dry weight. The oleaginous bacterium R. opacus strain proved useful for developing a new manufacturing paradigm to generate advanced lignocellulosic biofuels. United States. Defense Advanced Research Projects Agency (Grant HR0011-10-C-0187) Logos Technologies (Firm) MIT Energy Initiative Sweetwater Energy, Inc. 2015-04-27T17:32:58Z 2015-04-27T17:32:58Z 2014-06 2014-05 Article http://purl.org/eprint/type/JournalArticle 19485948 http://hdl.handle.net/1721.1/96819 Kurosawa, Kazuhiko, Sandra J. Wewetzer, and Anthony J. Sinskey. “Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus Opacus Strain for Lignocellulosic Biofuels.” Journal of Microbial & Biochemical Technology 06, no. 05 (2014). https://orcid.org/0000-0002-1015-1270 en_US http://dx.doi.org/10.4172/1948-5948.1000153 Journal of Microbial & Biochemical Technology Creative Commons Attribution http://creativecommons.org/ application/pdf OMICS Publishing Group Journal of Microbial & Biochemical Technology |
| spellingShingle | Kurosawa, Kazuhiko Wewetzer, Sandra J. Sinskey, Anthony J Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels |
| title | Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels |
| title_full | Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels |
| title_fullStr | Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels |
| title_full_unstemmed | Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels |
| title_short | Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels |
| title_sort | triacylglycerol production from corn stover using a xylose fermenting rhodococcus opacus strain for lignocellulosic biofuels |
| url | http://hdl.handle.net/1721.1/96819 https://orcid.org/0000-0002-1015-1270 |
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