Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells

Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells

Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformat...

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Bibliographic Details
Main Authors: Trifonova, Radiana, Jhunjhunwala, Siddharth, Hartoularos, George C., Eyerman, Alexandra T., Lytton-Jean, Abigail K. R., Angin, Mathieu, Sharma, Siddhartha, Poceviciute, Roberta, Mao, Shirley, Heimann, Megan, Liu, Sophia, Talkar, Tanya, Khan, Omar F., Addo, Marylyn M., von Andrian, Ulrich H., Langer, Robert, Lieberman, Judy, Jensen, Klavs F., Sharei, Armon Reza, Anderson, Daniel Griffith
Other Authors: Massachusetts Institute of Technology. Department of Chemical Engineering
Format: Article
Language:en_US
Published: Public Library of Science 2015
Online Access:http://hdl.handle.net/1721.1/97117
https://orcid.org/0000-0001-5629-4798
https://orcid.org/0000-0001-7192-580X
https://orcid.org/0000-0003-3811-2369
https://orcid.org/0000-0001-8046-2288
https://orcid.org/0000-0003-4255-0492
Description
Summary:Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies.

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