Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformat...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2015
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| Online Access: | http://hdl.handle.net/1721.1/97117 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0001-7192-580X https://orcid.org/0000-0003-3811-2369 https://orcid.org/0000-0001-8046-2288 https://orcid.org/0000-0003-4255-0492 |
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| author | Trifonova, Radiana Jhunjhunwala, Siddharth Hartoularos, George C. Eyerman, Alexandra T. Lytton-Jean, Abigail K. R. Angin, Mathieu Sharma, Siddhartha Poceviciute, Roberta Mao, Shirley Heimann, Megan Liu, Sophia Talkar, Tanya Khan, Omar F. Addo, Marylyn M. von Andrian, Ulrich H. Langer, Robert Lieberman, Judy Jensen, Klavs F. Sharei, Armon Reza Anderson, Daniel Griffith |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Trifonova, Radiana Jhunjhunwala, Siddharth Hartoularos, George C. Eyerman, Alexandra T. Lytton-Jean, Abigail K. R. Angin, Mathieu Sharma, Siddhartha Poceviciute, Roberta Mao, Shirley Heimann, Megan Liu, Sophia Talkar, Tanya Khan, Omar F. Addo, Marylyn M. von Andrian, Ulrich H. Langer, Robert Lieberman, Judy Jensen, Klavs F. Sharei, Armon Reza Anderson, Daniel Griffith |
| author_sort | Trifonova, Radiana |
| collection | MIT |
| description | Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies. |
| first_indexed | 2024-09-23T17:14:19Z |
| format | Article |
| id | mit-1721.1/97117 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T17:14:19Z |
| publishDate | 2015 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/971172022-10-03T11:18:22Z Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells Trifonova, Radiana Jhunjhunwala, Siddharth Hartoularos, George C. Eyerman, Alexandra T. Lytton-Jean, Abigail K. R. Angin, Mathieu Sharma, Siddhartha Poceviciute, Roberta Mao, Shirley Heimann, Megan Liu, Sophia Talkar, Tanya Khan, Omar F. Addo, Marylyn M. von Andrian, Ulrich H. Langer, Robert Lieberman, Judy Jensen, Klavs F. Sharei, Armon Reza Anderson, Daniel Griffith Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Ragon Institute of MGH, MIT and Harvard Koch Institute for Integrative Cancer Research at MIT Sharei, Armon Reza Jhunjhunwala, Siddharth Hartoularos, George C. Eyerman, Alexandra T. Lytton-Jean, Abigail K. R. Poceviciute, Roberta Mao, Shirley Heimann, Megan Liu, Sophia Talkar, Tanya Khan, Omar F. Anderson, Daniel Griffith Langer, Robert Jensen, Klavs F. Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies. National Institutes of Health (U.S.) (Grant R01GM101420-01A1) Kathy and Curt Marble Cancer Research Fund Ragon Institute of MGH, MIT and Harvard National Cancer Institute (U.S.) (Cancer Center Support (Core) Grant P30-CA14051) 2015-05-29T15:01:49Z 2015-05-29T15:01:49Z 2015-04 2014-11 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/97117 Sharei, Armon, Radiana Trifonova, Siddharth Jhunjhunwala, George C. Hartoularos, Alexandra T. Eyerman, Abigail Lytton-Jean, Mathieu Angin, et al. “Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells.” Edited by Philip A Stumbles. PLOS ONE 10, no. 4 (April 13, 2015): e0118803. https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0001-7192-580X https://orcid.org/0000-0003-3811-2369 https://orcid.org/0000-0001-8046-2288 https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.1371/journal.pone.0118803 PLOS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Trifonova, Radiana Jhunjhunwala, Siddharth Hartoularos, George C. Eyerman, Alexandra T. Lytton-Jean, Abigail K. R. Angin, Mathieu Sharma, Siddhartha Poceviciute, Roberta Mao, Shirley Heimann, Megan Liu, Sophia Talkar, Tanya Khan, Omar F. Addo, Marylyn M. von Andrian, Ulrich H. Langer, Robert Lieberman, Judy Jensen, Klavs F. Sharei, Armon Reza Anderson, Daniel Griffith Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells |
| title | Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells |
| title_full | Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells |
| title_fullStr | Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells |
| title_full_unstemmed | Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells |
| title_short | Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells |
| title_sort | ex vivo cytosolic delivery of functional macromolecules to immune cells |
| url | http://hdl.handle.net/1721.1/97117 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0001-7192-580X https://orcid.org/0000-0003-3811-2369 https://orcid.org/0000-0001-8046-2288 https://orcid.org/0000-0003-4255-0492 |
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