Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc
We report the synthesis and photophysical properties of ZBR4 and ZR1, two resorufin-based ditopic probes for mobile zinc. Upon binding Zn[superscript 2+], the sensors display 14- and 41-fold enhancements of their red fluorescence emission, respectively. In contrast to ZR1 and other members of the ZB...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Chemical Society (ACS)
2015
|
| Online Access: | http://hdl.handle.net/1721.1/97381 https://orcid.org/0000-0002-9269-7815 https://orcid.org/0000-0002-2693-4982 |
| _version_ | 1811072133460131840 |
|---|---|
| author | Radford, Robert J. Lippard, Stephen J. Loas, Andrei Ioan |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Radford, Robert J. Lippard, Stephen J. Loas, Andrei Ioan |
| author_sort | Radford, Robert J. |
| collection | MIT |
| description | We report the synthesis and photophysical properties of ZBR4 and ZR1, two resorufin-based ditopic probes for mobile zinc. Upon binding Zn[superscript 2+], the sensors display 14- and 41-fold enhancements of their red fluorescence emission, respectively. In contrast to ZR1 and other members of the ZBR family, which accumulate in the endoplasmic reticulum, ZBR4 spontaneously localizes to the mitochondria of HeLa cells. The modular approach in designing the constructs facilitates a homologation strategy aimed at tuning the zinc-binding and intracellular targeting properties of future probes. |
| first_indexed | 2024-09-23T09:01:26Z |
| format | Article |
| id | mit-1721.1/97381 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:01:26Z |
| publishDate | 2015 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/973812022-09-30T12:52:14Z Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc Radford, Robert J. Lippard, Stephen J. Loas, Andrei Ioan Massachusetts Institute of Technology. Department of Chemistry Loas, Andrei Ioan Radford, Robert J. Lippard, Stephen J. We report the synthesis and photophysical properties of ZBR4 and ZR1, two resorufin-based ditopic probes for mobile zinc. Upon binding Zn[superscript 2+], the sensors display 14- and 41-fold enhancements of their red fluorescence emission, respectively. In contrast to ZR1 and other members of the ZBR family, which accumulate in the endoplasmic reticulum, ZBR4 spontaneously localizes to the mitochondria of HeLa cells. The modular approach in designing the constructs facilitates a homologation strategy aimed at tuning the zinc-binding and intracellular targeting properties of future probes. National Institute of General Medical Sciences (U.S.) (Grant GM065519) 2015-06-11T12:21:43Z 2015-06-11T12:21:43Z 2014-06 2014-03 Article http://purl.org/eprint/type/JournalArticle 0020-1669 1520-510X http://hdl.handle.net/1721.1/97381 Loas, Andrei, Robert J. Radford, and Stephen J. Lippard. “Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc.” Inorg. Chem. 53, no. 13 (July 7, 2014): 6491–6493. © 2014 American Chemical Society https://orcid.org/0000-0002-9269-7815 https://orcid.org/0000-0002-2693-4982 en_US http://dx.doi.org/10.1021/ic500732z Inorganic Chemistry Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) ACS |
| spellingShingle | Radford, Robert J. Lippard, Stephen J. Loas, Andrei Ioan Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc |
| title | Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc |
| title_full | Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc |
| title_fullStr | Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc |
| title_full_unstemmed | Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc |
| title_short | Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc |
| title_sort | addition of a second binding site increases the dynamic range but alters the cellular localization of a red fluorescent probe for mobile zinc |
| url | http://hdl.handle.net/1721.1/97381 https://orcid.org/0000-0002-9269-7815 https://orcid.org/0000-0002-2693-4982 |
| work_keys_str_mv | AT radfordrobertj additionofasecondbindingsiteincreasesthedynamicrangebutaltersthecellularlocalizationofaredfluorescentprobeformobilezinc AT lippardstephenj additionofasecondbindingsiteincreasesthedynamicrangebutaltersthecellularlocalizationofaredfluorescentprobeformobilezinc AT loasandreiioan additionofasecondbindingsiteincreasesthedynamicrangebutaltersthecellularlocalizationofaredfluorescentprobeformobilezinc |