Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer
High-throughput production of nanoparticles (NPs) with controlled quality is critical for their clinical translation into effective nanomedicines for diagnostics and therapeutics. Here we report a simple and versatile coaxial turbulent jet mixer that can synthesize a variety of NPs at high throughpu...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2015
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| Online Access: | http://hdl.handle.net/1721.1/97488 https://orcid.org/0000-0003-0588-9286 https://orcid.org/0000-0003-1856-1925 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0003-1767-6296 |
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| author | Lim, Jong-Min Swami, Archana Gilson, Laura M. Chopra, Sunandini Choi, Sungyoung Langer, Robert Farokhzad, Omid C. Karnik, Rohit Wu, Jun, 1968- |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Lim, Jong-Min Swami, Archana Gilson, Laura M. Chopra, Sunandini Choi, Sungyoung Langer, Robert Farokhzad, Omid C. Karnik, Rohit Wu, Jun, 1968- |
| author_sort | Lim, Jong-Min |
| collection | MIT |
| description | High-throughput production of nanoparticles (NPs) with controlled quality is critical for their clinical translation into effective nanomedicines for diagnostics and therapeutics. Here we report a simple and versatile coaxial turbulent jet mixer that can synthesize a variety of NPs at high throughput up to 3 kg/d, while maintaining the advantages of homogeneity, reproducibility, and tunability that are normally accessible only in specialized microscale mixing devices. The device fabrication does not require specialized machining and is easy to operate. As one example, we show reproducible, high-throughput formulation of siRNA-polyelectrolyte polyplex NPs that exhibit effective gene knockdown but exhibit significant dependence on batch size when formulated using conventional methods. The coaxial turbulent jet mixer can accelerate the development of nanomedicines by providing a robust and versatile platform for preparation of NPs at throughputs suitable for in vivo studies, clinical trials, and industrial-scale production. |
| first_indexed | 2024-09-23T15:59:46Z |
| format | Article |
| id | mit-1721.1/97488 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:59:46Z |
| publishDate | 2015 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/974882022-09-29T17:33:25Z Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer Lim, Jong-Min Swami, Archana Gilson, Laura M. Chopra, Sunandini Choi, Sungyoung Langer, Robert Farokhzad, Omid C. Karnik, Rohit Wu, Jun, 1968- Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Koch Institute for Integrative Cancer Research at MIT Lim, Jong-Min Gilson, Laura M. Chopra, Sunandini Choi, Sungyoung Langer, Robert Karnik, Rohit High-throughput production of nanoparticles (NPs) with controlled quality is critical for their clinical translation into effective nanomedicines for diagnostics and therapeutics. Here we report a simple and versatile coaxial turbulent jet mixer that can synthesize a variety of NPs at high throughput up to 3 kg/d, while maintaining the advantages of homogeneity, reproducibility, and tunability that are normally accessible only in specialized microscale mixing devices. The device fabrication does not require specialized machining and is easy to operate. As one example, we show reproducible, high-throughput formulation of siRNA-polyelectrolyte polyplex NPs that exhibit effective gene knockdown but exhibit significant dependence on batch size when formulated using conventional methods. The coaxial turbulent jet mixer can accelerate the development of nanomedicines by providing a robust and versatile platform for preparation of NPs at throughputs suitable for in vivo studies, clinical trials, and industrial-scale production. Prostate Cancer Foundation (Award in Nanotherapeutics) National Institutes of Health (U.S.) (Grant EB015419) National Institutes of Health (U.S.) (Grant CA119349) 2015-06-22T13:45:14Z 2015-06-22T13:45:14Z 2014-05 2014-03 Article http://purl.org/eprint/type/JournalArticle 1936-0851 1936-086X http://hdl.handle.net/1721.1/97488 Lim, Jong-Min, Archana Swami, Laura M. Gilson, Sunandini Chopra, Sungyoung Choi, Jun Wu, Robert Langer, Rohit Karnik, and Omid C. Farokhzad. “Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer.” ACS Nano 8, no. 6 (June 24, 2014): 6056–6065. © 2014 American Chemical Society https://orcid.org/0000-0003-0588-9286 https://orcid.org/0000-0003-1856-1925 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0003-1767-6296 en_US http://dx.doi.org/10.1021/nn501371n ACS Nano Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) American Chemical Society |
| spellingShingle | Lim, Jong-Min Swami, Archana Gilson, Laura M. Chopra, Sunandini Choi, Sungyoung Langer, Robert Farokhzad, Omid C. Karnik, Rohit Wu, Jun, 1968- Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
| title | Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
| title_full | Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
| title_fullStr | Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
| title_full_unstemmed | Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
| title_short | Ultra-High Throughput Synthesis of Nanoparticles with Homogeneous Size Distribution Using a Coaxial Turbulent Jet Mixer |
| title_sort | ultra high throughput synthesis of nanoparticles with homogeneous size distribution using a coaxial turbulent jet mixer |
| url | http://hdl.handle.net/1721.1/97488 https://orcid.org/0000-0003-0588-9286 https://orcid.org/0000-0003-1856-1925 https://orcid.org/0000-0003-4255-0492 https://orcid.org/0000-0003-1767-6296 |
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