Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses
This is an author-corrected version of the final published paper.
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Springer-Verlag
2015
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| Online Access: | http://hdl.handle.net/1721.1/97734 https://orcid.org/0000-0002-9016-5962 |
| _version_ | 1811075698946736128 |
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| author | Son, Reuben S. Smith, Kyle C. Gowrishankar, Thiruvallur R. Vernier, P. Thomas Weaver, James C. |
| author2 | Institute for Medical Engineering and Science |
| author_facet | Institute for Medical Engineering and Science Son, Reuben S. Smith, Kyle C. Gowrishankar, Thiruvallur R. Vernier, P. Thomas Weaver, James C. |
| author_sort | Son, Reuben S. |
| collection | MIT |
| description | This is an author-corrected version of the final published paper. |
| first_indexed | 2024-09-23T10:10:31Z |
| format | Article |
| id | mit-1721.1/97734 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:10:31Z |
| publishDate | 2015 |
| publisher | Springer-Verlag |
| record_format | dspace |
| spelling | mit-1721.1/977342022-09-30T19:21:39Z Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses Son, Reuben S. Smith, Kyle C. Gowrishankar, Thiruvallur R. Vernier, P. Thomas Weaver, James C. Institute for Medical Engineering and Science Harvard University--MIT Division of Health Sciences and Technology Son, Reuben S. Smith, Kyle C. Gowrishankar, Thiruvallur R. Weaver, James C. This is an author-corrected version of the final published paper. Science increasingly involves complex modeling. Here we describe a model for cell electroporation in which membrane properties are dynamically modified by poration. Spatial scales range from cell membrane thickness (5 nm) to a typical mammalian cell radius (10 μm), and can be used with idealized and experimental pulse waveforms. The model consists of traditional passive components and additional active components representing nonequilibrium processes. Model responses include measurable quantities: transmembrane voltage, membrane electrical conductance, and solute transport rates and amounts for the representative “long” and “short” pulses. The long pulse—1.5 kV/cm, 100 μs—evolves two pore subpopulations with a valley at ~5 nm, which separates the subpopulations that have peaks at ~1.5 and ~12 nm radius. Such pulses are widely used in biological research, biotechnology, and medicine, including cancer therapy by drug delivery and nonthermal physical tumor ablation by causing necrosis. The short pulse—40 kV/cm, 10 ns—creates 80-fold more pores, all small (<3 nm; ~1 nm peak). These nanosecond pulses ablate tumors by apoptosis. We demonstrate the model’s responses by illustrative electrical and poration behavior, and transport of calcein and propidium. We then identify extensions for expanding modeling capability. Structure-function results from MD can allow extrapolations that bring response specificity to cell membranes based on their lipid composition. After a pulse, changes in pore energy landscape can be included over seconds to minutes, by mechanisms such as cell swelling and pulse-induced chemical reactions that slowly alter pore behavior. National Institutes of Health (U.S.) (Grant GM063857) National Science Foundation (U.S.) (Fellowship) Harvard University--MIT Division of Health Sciences and Technology (Fellowship) 2015-07-14T13:59:44Z 2015-07-14T13:59:44Z 2014-07 2014-04 Article http://purl.org/eprint/type/JournalArticle 0022-2631 1432-1424 http://hdl.handle.net/1721.1/97734 Son, Reuben S., Kyle C. Smith, Thiruvallur R. Gowrishankar, P. Thomas Vernier, and James C. Weaver. “Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses.” J Membrane Biol 247, no. 12 (July 22, 2014): 1209–1228. © 2014 Springer Science+Business Media New York https://orcid.org/0000-0002-9016-5962 en_US http://dx.doi.org/10.1007/s00232-014-9699-z http://hdl.handle.net/1721.1/96516 http://hdl.handle.net/1721.1/97707 The Journal of Membrane Biology Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Springer-Verlag Gowrishankar |
| spellingShingle | Son, Reuben S. Smith, Kyle C. Gowrishankar, Thiruvallur R. Vernier, P. Thomas Weaver, James C. Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses |
| title | Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses |
| title_full | Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses |
| title_fullStr | Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses |
| title_full_unstemmed | Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses |
| title_short | Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses |
| title_sort | basic features of a cell electroporation model illustrative behavior for two very different pulses |
| url | http://hdl.handle.net/1721.1/97734 https://orcid.org/0000-0002-9016-5962 |
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